Screening method of pumpkin material for glossy disease-resistant rootstock

A screening method and pumpkin technology, which is applied in the field of screening pumpkin materials for oily and disease-resistant rootstocks, to achieve the effects of improving screening accuracy, shortening the breeding period, and shortening the breeding time

Inactive Publication Date: 2017-09-01
青岛金妈妈农业科技有限公司
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  • Abstract
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  • Claims
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Problems solved by technology

[0012] Aiming at the problem of screening pumpkin rootstocks in the prior art, the purpose of the present invention is to provide a pumpkin material for oily and disease-resistant rootstock

Method used

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  • Screening method of pumpkin material for glossy disease-resistant rootstock
  • Screening method of pumpkin material for glossy disease-resistant rootstock

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Embodiment Construction

[0033] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0034] Materials used in the embodiments of the present invention are as follows:

[0035] PDA (Potato Dextrose Agar Medium): 200g potatoes, 20g glucose, 10g agar, 1L water, autoclaved at 121°C for 20min.

[0036] PDB (potato broth): 200g potatoes, 20g glucose, 1L water, autoclaved at 121°C for 20min.

[0037] Grading standards for Fusarium wilt disease: Grade 0: no browning at the base of the stem; Grade 1: browning at the base of the stem; Grade 2: browning at the base of 1 / 2 of the stem; Grade 3: browning at least 2 / 3 of the base of the stem; Grade 4: browning at the base of the stem All vascular bundles are browned to...

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Abstract

The invention relates to screening method of a pumpkin material for a glossy disease-resistant rootstock. The method comprises the following steps: (1) grafting: collecting different pumpkin germplasm materials, grafting cucumber scions to pumpkin seedlings having two true leaves and an apical bud, pinching the true leaves of rootstock pumpkins before the grafting, retaining growing points, planting grafted seedlings, and retaining two vines, a cucumber vine and a pumpkin vine; (2) cucumber glossy performance screening: comparing and screening the grafted seedlings, selecting materials with the most obvious cucumber dewaxing powder and the best brightness, after bearing 2 to 3 cucumbers, removing the cucumber vines in time, only retaining the pumpkin vines to identify and screen disease resistance character or reserve seeds; (3) pumpkin rootstock disease resistance screening: screening the grated seedlings for resistance to fusarium wilt, resistance to powdery mildew, and resistance to CGMMV, selfing the materials with resistance to disease and cucumber glossy performance and retaining F1 generation pumpkin seeds. The method has short breeding time, high efficiency and standardized means, and can retain the fine properties of pumpkin seedling and rootstock at the same time.

Description

technical field [0001] The invention belongs to the field of plant screening and breeding, and in particular relates to a method for screening pumpkin materials for oily and disease-resistant stock. Background technique [0002] Pumpkin resources are rich and diverse, but my country has not made a systematic identification and evaluation of many excellent pumpkin germplasm resources, and the utilization rate of materials is not high. [0003] In recent years, with the promotion of grafting technology and public recognition, the planting area of ​​grafted cucumbers is also expanding, and the grafting diseases caused by large-scale planting and continuous cropping are also becoming more and more serious. However, research on resistance to major diseases of pumpkin such as viral diseases, fusarium wilt, and powdery mildew lags behind production. [0004] Since the 1980s, some domestic breeding units have started research on the breeding of pumpkins for rootstocks. At present, ...

Claims

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Application Information

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IPC IPC(8): A01H1/04A01G1/06A01G7/06
CPCA01H1/04A01G7/06
Inventor 王忠全周玉忠陈玉梅栗文霞刘蕾庆刘培培初敏刘凌云闻凤娟程松莲单泊仁
Owner 青岛金妈妈农业科技有限公司
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