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Anoectochilus roxburghii tissue culture seedling method

A technology of tissue culture and culture medium, which is applied in the field of clematis tissue culture and seedlings, can solve the problems that the production of clematis cannot keep up with the market demand, high cost, long culture time, etc., and achieve shortening of production cycle and operation steps The effect of reduction and labor cost reduction

Active Publication Date: 2015-12-02
GUANGDONG HUXINGSHAN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After many years of tissue culture practice, tissue culture and rapid propagation technology of A. aureus is also developing day by day. However, at this stage, the tissue culture technology mainly focuses on improving the adventitious bud induction rate and bud proliferation coefficient, etc., and there are still problems such as long culture time and high cost. , the output of clematis has been unable to keep up with the demand of the market

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045]A. Weigh 200g of activated carbon, 2000g of sucrose and 16kg of sweet potato puree, add 100L of selenium-enriched pure water and mix well and heat to boiling. After stopping heating, add MS mother liquor, and add 100g of peptone, 600g of agar, 85g of Huabao No. 1, Huabao Bao No. 4 20g and Huabao No. 5 25g, the pH value of the mixed culture medium was adjusted to 5.6, and after the agar was completely dissolved, it was divided into culture bottles while it was hot.

[0046] For sterilization, place the culture bottle filled with culture medium in a high-temperature and high-pressure environment at 120°C and 160kPa for 15 minutes to sterilize, take out the culture bottle after sterilization, and cool it for later use.

[0047] B, inoculation, select the robust wild clematis plants that grow naturally and have no damage by diseases and insect pests, move them into a clean shed and cultivate them for 7 days, take out the cultivated plants, wash them with running water, remove...

Embodiment 2

[0052] A. Weigh 300g of activated carbon, 2500g of sucrose and 15kg of sweet potato puree, add 100L of selenium-enriched pure water, mix well and heat to boiling, stop heating, add MS mother liquor, and add 200g of peptone, 650g of agar, 80g of Huabao No. 1, Huabao Bao No. 4 30g and Huabao No. 5 20g, the pH value of the mixed culture medium was adjusted to 5.6, after the agar was completely dissolved, it was divided into culture bottles while it was hot.

[0053] For sterilization, place the culture bottle filled with culture medium in a high-temperature and high-pressure environment at 123°C and 150kPa for 18 minutes to sterilize, take out the culture bottle after sterilization, and cool it for later use.

[0054] B, inoculation, select the robust wild clematis plants that grow naturally and have no damage by diseases and insect pests, move them into a clean shed and cultivate them for 7 days, take out the cultivated plants, wash them with running water, remove surface attachm...

Embodiment 3

[0059] A. Weigh 400g of activated carbon, 3000g of sucrose and 12kg of sweet potato puree, add 100L of selenium-enriched pure water and mix well and heat to boiling. After stopping heating, add MS mother liquor, and add 300g of peptone, 700g of agar, 85g of Huabao No. 1, Huabao Bao No. 4 10g and Huabao No. 5 30g, the pH value of the mixed culture medium was adjusted to 5.6, and after the agar was completely dissolved, it was divided into culture bottles while it was hot.

[0060] For sterilization, place the culture bottle filled with culture medium in a high-temperature and high-pressure environment at 125°C and 140kPa for 20 minutes to sterilize, take out the culture bottle after sterilization, and cool it for later use.

[0061] B, inoculation, select the robust wild clematis plants that grow naturally and have no damage by diseases and insect pests, move them into a clean shed and cultivate them for 7 days, take out the cultivated plants, wash them with running water, remov...

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Abstract

The present invention discloses an anoectochilus roxburghii tissue culture seedling method, mainly comprises: A. culture medium preparing, wherein the step A comprises two steps of preparation and sterilization; B. inoculating, wherein a pre-treated explantation is defoliated under a sterile condition, a base stem segment with one knob is cut from each explantation and inoculated on the prepared culture medium; and C. culturing, comprising culturing in dark and culturing in light. According to the present invention, mashed sweet potato is coordinated with Hyponex 1, Hyponex 4 and Hyponex 5, the culture medium is prepared by a reasonable proportion to promote inducement and growth of anoectochilus roxburghii seedlings; the seedlings in a culture flask is free from transfer, thus operation steps are reduced, labour cost is lowered, a contamination rate is reduced, and a seedling rate is high, wherein a bud occurs within 15 days, rooting occurs after one month, and a common tissue culture seedling is produced after four months; the seedling rate is over 94%, and the survival rate of transplantation after two months is over 97%; and therefore a production period of the anoectochilus roxburghii seedling is shortened, and a rapid seedling culturing is prompted.

Description

technical field [0001] The invention relates to the technical field of clematis cultivation, in particular to a method for growing seedlings of clematis tissue culture. Background technique [0002] Clematis clematis is a perennial rare Chinese herbal medicine of the Orchidaceae lip plant. It has a wide range of folk use and has the effect of curing all kinds of diseases. It is known as "the king of medicine", "golden grass", "miracle medicine", "bird ginseng", etc. It is known as the southern Cordyceps sinensis and is a rare wild mountain treasure. , of extraordinary value. Since the Tang Dynasty, it has been listed as a tribute and entered the court, and it has been a special royal product for the imperial palaces of all dynasties. [0003] "Compendium of Materia Medica", "Chinese Materia Medica", "Supplements to Materia Medica", "Dictionary of Traditional Chinese Medicine", "Fujian Drugs", "Chinese Economic Flora", "New Compilation of Materia Medica in South Zhejiang" a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 邹林江
Owner GUANGDONG HUXINGSHAN BIOTECH
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