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Embedding, germination accelerating and germinating method of high-mountain miniature potatoes in Huaiyu shan

A technology of miniature tubers and potatoes, which is applied in the field of agricultural science and can solve problems such as weak growth, low emergence rate, and affecting seed reproduction yield.

Inactive Publication Date: 2020-10-27
SHANGRAO NORMAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] However, potato microtubers are small in size, light in weight, low in starch and other nutrients and energy substances, and their growth is weak, and their emergence rate is low. It is easy to cause water evaporation and loss during storage, which often affects germination and emergence, and even reduces the vigor of seed potatoes. The above problems have become the main obstacles to the development of seed potato production

Method used

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  • Embedding, germination accelerating and germinating method of high-mountain miniature potatoes in Huaiyu shan

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Embodiment 1

[0029] A method for embedding and accelerating germination of Huaiyu Mountain alpine potato miniature tubers, comprising the following steps:

[0030] (1) Soak Huaiyu Mountain Alpine Potato Microtubers in MS culture solution containing 0.05g / L gibberellin for 24 hours at 25°C;

[0031] (2) Soak Huaiyu Mountain Alpine Potato Microtuber in MS culture solution containing 0.05g / L chitooligosaccharide for 24h at 25°C;

[0032] (3) Under 25°C, the Huaiyushan alpine potato microtubers were soaked in MS culture solution containing 0.4g / L graphene quantum dots for 24h;

[0033] (4) Soak Huaiyu Mountain Alpine Potato Microtubers in MS culture solution containing 0.4g / L carbon nanotubes for 24 hours at 25°C;

[0034] (5) Mix the soaked potato minitubers with the modified MS culture solution containing 0.03g / L sodium alginate and 0.2mol / L sucrose at 25°C to obtain a minituber mixture, drop the minituber mixture into a mixture containing 0.05g / L L gibberellin, 0.05g / L chitosan oligosacch...

Embodiment 2

[0038] A method for embedding and accelerating germination of Huaiyu Mountain alpine potato miniature tubers, comprising the following steps:

[0039] (1) Soak Huaiyu Mountain Alpine Potato Microtubers in MS culture solution containing 0.05g / L gibberellin for 24 hours at 24°C;

[0040] (2) Soak Huaiyu Mountain alpine potato miniature tubers in MS culture solution containing 0.05g / L chitooligosaccharide for 24 hours at 26°C;

[0041] (3) Soak Huaiyu Mountain alpine potato microtubers in MS culture solution containing 0.5g / L graphene quantum dots for 24 hours at 24°C;

[0042] (4) Soak Huaiyu Mountain alpine potato microtubers in MS culture solution containing 0.3g / L carbon nanotubes for 24 hours at 26°C;

[0043] (5) Mix the soaked potato minitubers with the modified MS culture solution containing 0.03g / L sodium alginate and 0.2mol / L sucrose at 26°C to obtain a minituber mixture, drop the minituber mixture into a mixture containing 0.05g / L L gibberellin, 0.05g / L chitosan olig...

Embodiment 3

[0047] A method for embedding and accelerating germination of Huaiyu Mountain alpine potato miniature tubers, comprising the following steps:

[0048] (1) Soak Huaiyu Mountain Alpine Potato Microtubers in MS culture solution containing 0.05g / L gibberellin for 24 hours at 26°C;

[0049] (2) Soak Huaiyu Mountain alpine potato miniature tubers in MS culture solution containing 0.05g / L chitooligosaccharide for 24 hours at 24°C;

[0050] (3) Soak Huaiyu Mountain Alpine Potato Microtubers in MS culture solution containing 0.3g / L graphene quantum dots for 24 hours at 26°C;

[0051] (4) Soak Huaiyu Mountain alpine potato microtubers in MS culture solution containing 0.5g / L carbon nanotubes for 24 hours at 24°C;

[0052] (5) Mix the soaked potato minitubers with the modified MS culture solution containing 0.03g / L sodium alginate and 0.2mol / L sucrose at 24°C to obtain a minituber mixture, drop the minituber mixture into a mixture containing 0.05g / L L gibberellin, 0.05g / L chitosan oligos...

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Abstract

The invention discloses an embedding, germination accelerating and germinating method of high-mountain miniature potatoes in Huaiyu shan, and belongs to the field of agricultural science. The method comprises the following steps: firstly, soaking the miniature potatoes in MS culture solutions containing different components, mixing the potatoes with an improved MS culture solution, then embeddingthe potatoes, and carrying out culturing under special conditions to enable the miniature potatoes to germinate. According to the method, through soaking and embedding culture, germination of the Huaiyu shan high-mountain miniature potatoes can be effectively promoted, the germination rate of the Huaiyu shan high-mountain miniature potatoes is greatly increased, and meanwhile germination and seedling formation of embedded beads of the Huaiyu shan high-mountain miniature potatoes are promoted through irrigation of the MS culture solution and alternate culture with day and night temperature difference; and therefore, the germination rate of the embedded beads of the Huaiyu shan high-mountain miniature potatoes is effectively increased, plants are promoted to be robust, sufficient nutrients can be provided, and follow-up culture and transplanting survival are facilitated.

Description

technical field [0001] The invention relates to the field of agricultural science, in particular to a method for embedding and accelerating germination of Huaiyu Mountain alpine potato microtubers. Background technique [0002] Potato mini tubers refer to small tubers with a diameter of 1 to 10 cm produced by transplanting virus-free test tube seedlings or cutting stems in the artificially prepared nutrient matrix in the insect-proof greenhouse. The industrial production of mini-potatoes is a new technology for large-scale and high-standard production of virus-free seed potatoes by combining soilless cultivation technology, plant tissue culture technology, aeroponic technology and cutting rapid propagation technology. Due to the small size and the weight of only a few grams, the micro-potatoes are convenient for mass transportation, which solves the problem of difficulty in transportation of potato seeds. Therefore, the production of microtubers has developed rapidly and ha...

Claims

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Application Information

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IPC IPC(8): A01C1/06A01C1/00A01G22/25C05G3/00C05G5/20
CPCA01C1/00A01C1/06A01G22/25C05D3/00C05D9/00C05F11/00C05F11/10C05G3/00C05G5/20
Inventor 洪森荣尹明华曾晓健
Owner SHANGRAO NORMAL UNIV
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