The invention provides a compound feed of a mature trionyx sinensis and a preparation method thereof. The compound feed comprises fish meal, alpha-starch, fermented soya bean meal, fish oil, yeast, methionine, lysine, milk powder, yolk powder, compound mineral premix for trionyx sinensis feeds, compound vitamin premix for the trionyx sinensis feeds, monocalcium phosphate, enzymic preparations and Chinese medicinal herb additives. The preparation process comprises the following steps of: charging materials; initially cleaning; mixing for the first time; removing iron; smashing; mixing for the second time; and packaging. The invention has scientific and reasonable collocation of a feed formula, enhances the disease resistance of the mature trionyx sinensis, selects feed raw materials with high digestive absorption rate, enhances the conversion rate of the feed, decreases the diseases of the mature trionyx sinensis by reducing the discharge of pollutants, i.e. nitrogen, phosphorus, and the like, and the pollution of culturing water bodies, finally meets the requirements for ecological culture by reducing medicine dosage, enhances the absorption utilization rate of the feed, and can meet the nutritional requirements of the mature trionyx sinensis, reduce the culture cost and also reduce the pollution of the water bodies; in addition, the preparation method is scientific and reasonable and has outstanding economic benefit and environmental benefit.