Microbial agent and preparation method and application thereof

A microbial agent, Streptomyces technology, applied in botany equipment and methods, chemicals for biological control, applications, etc., can solve problems such as economic loss, intolerance to storage, and small damage area, and achieve environmental protection problems, reduce disease occurrence, and improve soil quality

Inactive Publication Date: 2017-12-05
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Usually, although the disease spots are limited to the cortex, the quality and yield of the damaged potato pieces can still be reduced, and they are not resistant to storage, and the appearance of the diseased potato is unsightly, and the commodity grade is greatly reduced, resulting in certain economic losses.
In the past, due to the low incidence rate and small damage area in our country, it did not attract people's attention.

Method used

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  • Microbial agent and preparation method and application thereof
  • Microbial agent and preparation method and application thereof
  • Microbial agent and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The preparation method of microbial bacterial agent:

[0021] 1) Preparation of potato solid medium (g / L): 1L of potato extract, 20g of glucose, natural pH; add 15g of agar to the solid medium;

[0022] 2) Bacillus subtilis AS 1.398 was activated 3 times on potato solid medium;

[0023] 3) Take 1 ring in 50ml potato liquid medium (which medium is it referring to, is it the same as the above-mentioned solid medium), at 28°C, and culture on a shaker for 2 days;

[0024] 4) After the fermentation broth was centrifuged at 12,000 r / min for 1 minute, the supernatant was discarded, and the precipitate was suspended in the same volume of sterile water to prepare a cell suspension.

Embodiment 2

[0026] Preparation of medium:

[0027] 1) Potato medium (g / L): 1L of potato extract, 20g of glucose, natural pH; add 15g of agar to the solid medium.

[0028] 2) ISP-2 medium (g / L): 4 g of yeast extract, 10 g of malt extract, 4 g of glucose, pH 7.3, 15 g of agar was added.

Embodiment 3

[0029] Example 3 Bacillus subtilis (Bacillus subtilis) AS 1.398 cultivation method: Bacillus subtilis (Bacillus subtilis) AS 1.398 was activated 3 times on potato solid medium. Then take 1 ring and put it in 50ml potato liquid medium, culture it on a shaker at 28°C for 2 days. After the fermentation broth was centrifuged at 12,000r / min for 1 minute, the supernatant was discarded, and the precipitate was suspended in the same volume of sterile water to prepare a cell suspension.

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Abstract

The invention relates to the field of biopharmacy, specifically to a preparation method and application of a microbial agent. An active component of the microbial agent is bacillus subtilis AS1.398. The microbial agent of the invention has a good effect of controlling deep scab of potato and can be used for biological control of deep scab of potato.

Description

technical field [0001] The invention relates to a microbial bacterial agent and a preparation method thereof, in particular to a microbial bacterial agent with bacillus subtilis as an active ingredient and a preparation method thereof. Background technique [0002] Potato scab is a disease of potato tubers. It is manifested by the appearance of nearly round to amorphous suberized scab-like light brown lesions or plaques on the surface of the tuber, and the texture is rough to the touch. It is generally divided into two types of onset symptoms, which are reticulate lesions and cleft-like lesions. Usually, although the diseased spots are limited to the cortex, the quality and yield of the damaged potato pieces can still be reduced, and they are not resistant to storage, and the appearance of the diseased potato is unsightly, and the commodity grade is greatly reduced, resulting in certain economic losses. In the past, due to the low incidence rate and small damage area in ou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/02A01P1/00
CPCA01N63/10
Inventor 仲乃琴钞亚鹏张国青赵盼杨敬
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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