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Method for locally culturing and producing arbuscular mycorrhiza fungus biological agent fertilizer in farmlands

A technology of arbuscular mycorrhizal fungus and biological agent, applied in organic fertilizers, climate change adaptation, etc., to achieve the effects of reduced dosage, simple cultivation, and easy material acquisition

Inactive Publication Date: 2010-08-18
ZHEJIANG NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In addition, current biofertilizers mainly focus on bacteria, such as rhizobia, nitrogen-fixing bacteria, silicate bacteria, phosphorus and potassium solubilizers, and there is no symbiotic fungal biofertilizer

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] 1) Production of farmland culture ponds and culture substrates

[0035]In the farmland, use polyethylene plastic film and wooden stakes to surround the cultivation pond, the distance between the wooden piles is 60cm * 60cm, the surroundings and the bottom of the cultivation pond are surrounded by plastic film, the volume of the cultivation pond is 60cm * 60cm * 20cm, and then according to The volume of the culture pool is added to the culture pool by adding 15 parts of hot water, 50 parts of vermiculite, and 25 parts of humus soil. Purchase, humus soil is obtained after accumulation and decay of soil and leaves and branches;

[0036] 2) Transplantation of arbuscular mycorrhizal symbiosis and its cultivation

[0037] The symbiont between the host plant and the arbuscular mycorrhizal fungus: make a culture container, take a small stainless steel bucket, the size is 6.0×1.1×22.5cm 3 (upper opening diameter × lower opening diameter × height), there is a hole at the bottom...

Embodiment 2

[0051] In step 1), 5 parts of hot water, 40 parts of vermiculite, and 20 parts of humus soil are adopted; in step 2), the host root infection rate is adopted to reach more than 80%, and the number of spores reaches 20 / g. During this period, pour 15ml of nutrient solution once a week, and pour 10ml of root leaching stock solution, 15ml of compound amino acid nutrient solution, and 5ml of ammonium humate solution with a concentration of 2% in the surrounding culture medium of each plant symbiont at the same time. strain, the nutrient solution formula used is: KNO 3 500mg / L, Ca(NO 3 ) 2 720mg / L, MgSO 4 360mg / L,H 3 BO 3 6mg / L, MnCl 2 .4H 2 O 5mg / L, ZnSO 4 .7H 2 O 5mg / L, CuSO 4 .5H 2 O 1mg / L, H 2 MoO 4 .H 2 O 1mg / L, do not add phosphate; step 3) in the symbiont of host plant and arbuscular mycorrhizal fungus after cultivating 6-8 weeks in culture tank, use 500 order nylon nets to culture tank at 12cm away from host plant Separated into two chambers; step 4) biologi...

Embodiment 3

[0053] Step 1) adopts 10 parts of hot water, 60 parts of vermiculite, and 25 parts of humus soil; step 2) adopts the symbiont whose host root infection rate reaches more than 70%, and the number of spores reaches 15 / g, and cultivates During this period, pour 25ml of nutrient solution once a week, and pour 25ml of root extract stock solution, 25ml of compound amino acid nutrient solution, and 2ml of ammonium humate solution with a concentration of 3% in the surrounding culture medium of each plant symbiont at the same time. strain, the nutrient solution formula used is: KNO 3 300mg / L, Ca(NO 3 ) 2 520mg / L, MgSO 4 160mg / L,H 3 BO 3 3mg / L, MnCl 2 .4H 2 O 1mg / L, ZnSO 4 .7H 2 O 1mg / L, CuSO 4 .5H 2 O 0.05mg / L, H 2 MoO 4 .H 2 O 0.05mg / L, do not add phosphate; step 3) in the host plant and the symbiont of arbuscular mycorrhizal fungus after cultivating 7 weeks in the culture pond, separate the culture pond with 400 order nylon nets at 15cm away from the host plant into ...

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Abstract

The invention provides a method for locally culturing and producing arbuscular mycorrhiza fungus biological agent fertilizer in farmlands. The method is characterized by comprising the following steps of: 1, preparing a farmland culturing pond and a culture medium; 2, transplanting and culturing arbuscular mycorrhiza fungi; 3, absorbing and converting biological fertilizer; and 4, collecting biological fertilizer. Compared with the prior art, the technique adopted in the method has the advantages that: 1, the raw materials are readily available, the culture of the raw materials is easy, and the cost is low; 2, the culture technique and the production technique are conveniently popularized in rural areas; and 3, the production technique is green and environment friendly, which facilitates the development of modern agriculture. In addition, by using the fertilizer of the invention, the used amount of the quick-acting fertilizer is reduced and the used amount of partial fertilizer is reduced, so that the pollution to soil, water resources and air is lowered, and the fertilizer is particularly suitable to be used for planting seedlings of farm crops and medicinal plants. Tests show that the yield of the farm crops cultured by using the fertilizer of the method is improved by over 50 percent compared with that of the farm crops cultured by using the common fertilizer.

Description

technical field [0001] The invention belongs to the technical field of biological fertilizer production, and in particular relates to a method for producing arbuscular mycorrhizal fungus biological agent fertilizer by in-situ cultivation in farmland. Background technique [0002] Arbuscular mycorrhizal fungi (AMF) are ancient and widespread symbionts that have a symbiotic relationship with more than 80% of land plants. In this symbiotic system, the sugars synthesized by plants through photosynthesis can be transported to the endophytic hyphae of AM fungi (usually hexose sugars), and the endophytic hyphae synthesize hexose sugars into lipids, which are then transported to the exophytic hyphae for the growth and reproduction of the bacteria. AM fungi extend mycelium into the soil, thereby expanding the absorption surface of plants, which can improve the absorption of soil nutrients, such as P, N, S, Zn and water, and enhance plant resistance to disease and drought ability. ...

Claims

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Application Information

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IPC IPC(8): C05F11/08
CPCY02A40/10
Inventor 金海如马继芳田萌萌
Owner ZHEJIANG NORMAL UNIVERSITY
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