Timed and circulating AM (arbuscular mycorrhizal) fungus expanding propagation and functional experiment device and application method thereof

Abstract

The invention provides a timed and circulating AM (arbuscular mycorrhizal) fungus expanding propagation and functional experiment device and relates to culture of soil microorganisms. The device is provided with an incubator cover, an incubator, a culture medium, a space for adding an isotope and the like, a hypha chamber, a water drainage tank cover, a water drainage tank, a filter pump, a plant growth chamber, a nozzle regulation and control hook and a nylon net, wherein the incubator cover covers the incubator, the incubator is divided into the hypha chamber and the plant growth chamber by virtue of a longitudinal net, the culture medium and the space for adding the isotope and the like are placed in the hypha chamber, and a water drainage hole is formed in the bottom of the incubator; the water drainage tank is arranged at the bottom of the incubator, the water drainage tank cover covers the water drainage tank, the filter pump is arranged in the water drainage tank, the nozzle regulation and control hook and the nylon net are arranged in the plant growth chamber, an atomizing nozzle is formed in the nozzle regulation and control hook, the atomizing nozzle and the filter pump are connected by virtue of a PVC hose, and a plant fixing device is arranged on the top of the plant growth chamber.

Description

technical field [0001] The invention relates to the cultivation of soil microorganisms, in particular to a timed cycle AM ​​fungus propagation and functional experiment device and usage method. Background technique [0002] Arbuscular mycorrhizae (AM) fungi are one of the most widely distributed soil microorganisms in nature, and they can form mycorrhizal symbionts with more than 80% of terrestrial plants on the earth. Different levels have important functions, but these potentially huge roles have not been fully exploited. Because AM fungi are obligate biotrophic microorganisms, asexual reproduction has rich genetic diversity, so far they cannot be completely cultured in vitro, and can only rely on living plants to reproduce them. This has become a taxonomic identification of AM fungi and The main barrier for a wide range of applications. The reason is that the physiology of mycorrhizal symbionts and the relationship between fungi and hosts in the symbiont system have not...

AI Technical Summary

Problems solved by technology

The biggest disadvantage of this patent is that the host material is that the root system of the plant cannot perform photosynthesis to produce photosynthetic products, which violates the symbiotic relationship between the plant and the host plant in the natural environment

To sum up, the cultivation devices designed in the above patents are basically aimed at improving the culture medium and optimizing the AM fungal agent, but there are few studies that combine the propagation of the agent with the research on the functional characteristics of the AM fungus

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