Cell culture container and shaking table

Abstract

The invention discloses a cell culture container which comprises a container body, a connecting plate is arranged in the container body, and a plurality of through holes for cell sap to pass through are distributed in the connecting plate. The invention further discloses a shaking table which comprises a movable platform, a platform driver and the cell culture container, the container body is arranged on the movable platform, and the platform driver is used for driving the movable platform. The cell culture container and the shaking table have the advantages of being suitable for large-scale culture, capable of timely and uniformly stirring cell sap and the like.

Description

Technical Field

[0001] The invention relates to the technical field of cell culture, and in particular to a cell culture container and a shaking table. Background Art

[0002] The bioprocessing industry routinely uses stainless steel container systems and piping in manufacturing processes for fermentation and cell culture. Due to cost considerations, such containers are designed to be steam sterilized and reused. Plastic disposable containers (rigid or flexible) and tubes are also used. Such disposable culture containers are typically provided sterile, eliminating the need for user-side cleaning and sterilization steps, minimizing the risk of contamination.

[0003] However, in large-scale culture, it is difficult to ensure sufficient mixing of the suspended cell fluid during the culture process by simply using a rocking motion, such as a shaker, to place a disposable container on a shaker platform and provide dissolved oxygen to the cell fluid through rocking. It is also difficult to ensure that the cells in a large number of cell suspensions can be fully contacted and ventilated with the gas in a timely and uniform manner.

[0004] For example, 201980027891.0 is an inflatable bioreactor and method of use, in which a disturbance protrusion is provided in the bag, and the disturbance protrusion can be configured to provide oxygenation necessary for initial low-volume culture. During operation of the bioreactor system, the disturbance protrusion can stir the small initial volume of the cell culture medium by causing a "weir"-like effect when the volume of the cell culture is small, but has a smaller effect when the volume of the culture medium is large. In other words, the disturbance protrusion is not suitable for larger-scale culture and cannot stir the cell fluid in a timely and uniform manner. Summary of the invention

[0005] The technical problem to be solved by the present invention is to overcome the deficiencies of the prior art and provide a cell culture container and a shaking table which are suitable for large-scale culture and can stir the cell fluid in a timely and uniform manner.

[0006] In order to solve the above technical problems, the present invention adopts the following technical solutions:

[0007] A cell culture container comprises a container body, wherein a connecting plate is arranged in the container body and a plurality of through holes for cell fluid to pass through are distributed on the connecting plate.

[0008] As a further improvement of the above technical solution:

[0009] The container body comprises a bottom film and a top film, and the bottom film and the top film are combined to form a flexible container body.

[0010] The connecting plate is flexible or foldable.

[0011] Both ends of the connecting plate are provided with fixing parts fixedly connected to the container body.

[0012] The middle part of the connecting plate is provided with a fixing part which is fixedly connected with the container body.

[0013] The container body is provided with a plurality of interfaces, a dissolved oxygen sensor and a pH sensor.

[0014] The container body is a rigid structure.

[0015] The bottom surface of the container body is polygonal, circular, elliptical or waist-shaped.

[0016] The connecting plate is provided with one and is located in the middle of the container body.

[0017] There are at least two connecting plates, which are arranged at intervals along the length direction of the container body.

[0018] There are at least two connecting plates, which are staggered along the length and width directions of the container body.

[0019] A rocking table comprises a movable platform, a platform drive and the above-mentioned cell culture container, wherein the container body is arranged on the movable platform, and the platform drive is used for driving the movable platform.

[0020] As a further improvement of the above technical solution:

[0021] The platform drive can drive the movable platform to swing around at least one axis within a certain angle range or drive the movable platform to rotate.

[0022] Compared with the prior art, the advantages of the present invention are:

[0023] The cell culture container of the present invention, when in use, contains a cell suspension in the container body, and the container body is fixed on a swinging platform of a device (shaking table), and the container body is swung back and forth. During the swinging process of the container body to culture cells, the cell suspension in the container body reciprocates through the through holes on the connecting plate, thereby achieving full disturbance of the cell suspension, making the cells in the cell suspension evenly distributed and fully in contact with the air in the container body, so as to improve the cell culture efficiency. Since the connecting plate has the above functions, the cell culture container is suitable for large-scale culture, and can stir the cell fluid in a timely and uniform manner.

[0024] The shaking table of the present invention comprises all the technical features of the cell culture container and has all the advantages of the cell culture container. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 It is a schematic diagram of the three-dimensional structure of the first embodiment of the cell culture container of the present invention.

[0026] Figure 2 It is a schematic structural diagram of the internal connecting plate of the first embodiment of the cell culture container of the present invention.

[0027] Figure 3 1 is a diagram of the folded state of the first embodiment of the cell culture container of the present invention.

[0028] Figure 4 This is a diagram of the folded state of the connecting plate of the first embodiment of the cell culture container of the present invention.

[0029] Figure 5 It is a schematic diagram of the three-dimensional structure of the second embodiment of the cell culture container of the present invention.

[0030] Figure 6 It is a schematic diagram of the three-dimensional structure of the third embodiment of the cell culture container of the present invention.

[0031] Figure 7 It is a schematic structural diagram of the internal connection plate of the third embodiment of the cell culture container of the present invention.

[0032] Figure 8 It is a structural schematic diagram of an embodiment of a rocking table of the present invention.

[0033] Fig. 9 It is a structural schematic diagram of another embodiment of the rocking table of the present invention.

[0034] The symbols in the figure represent:

[0035] 1. Container body; 11. Bottom membrane; 12. Top membrane; 121. Interface; 13. Side membrane; 2. Connecting plate; 3. Through hole; 4. Internal space; 5. Dissolved oxygen sensor; 6. PH sensor; 7. Movable platform. DETAILED DESCRIPTION

[0036] The present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments.

[0037] In the description of the present application, it should be understood that the terms "length", "width", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", etc., indicating the orientation or position relationship, are based on the orientation or position relationship shown in the drawings, and are only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present application.

[0038] In addition, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of the features. In the description of this application, the meaning of "plurality" is two or more, unless otherwise clearly and specifically defined.

[0039] In this application, unless otherwise clearly specified and limited, the terms "assemble", "connect", "connect", "fix" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in this application can be understood according to specific circumstances.

[0040] Embodiment 1:

[0041] Figure 1 and Figure 4 An embodiment of a cell culture container of the present invention is shown. The cell culture container of this embodiment includes a container body 1, a connecting plate 2 is provided in the container body 1, and a plurality of through holes 3 for cell fluid to pass through are distributed on the connecting plate 2. When in use, a cell suspension is loaded in the container body 1, and the container body 1 is fixed on the swing tray of the equipment, and the container body 1 is swung back and forth. During the swinging process of the container body 1 to culture cells, the cell suspension in the container body 1 reciprocates through the through holes 3 on the connecting plate 2, so that the cell suspension is fully disturbed, so that the cells in the cell suspension are evenly distributed and fully contacted with the air in the container body 1, so as to improve the cell culture efficiency. Since the connecting plate 2 has the above functions, the cell culture container is suitable for large-scale culture, and can stir the cell fluid in a timely and uniform manner.

[0042] Furthermore, in this embodiment, the container body 1 includes a bottom film 11 and a top film 12, and the bottom film 11 and the top film 12 enclose the flexible container body 1. As shown in the figure, the two sides of the bottom film 11 are folded to form side films 13, so that as few heat-sealed gaps as possible appear at the bottom of the container body 1, which can avoid cell residues, and the heat-sealed gaps may hinder the flow of cell fluid. Furthermore, the two ends of the bottom film 11 and the top film 12 are heat-sealed and connected, and a fixing strip can be provided at the connection between the two.

[0043] Furthermore, in this embodiment, both ends of the connecting plate 2 are provided with fixing parts fixedly connected to the container body 1, so as to improve the stability of the connecting plate 2, and when the container body 1 is shaken, the connecting plate 2 can be prevented from being worn due to relative movement in the container body 1, causing foreign matter contamination, etc. Of course, in other embodiments, the two ends of the connecting plate 2 may not be fixedly connected to the two side membranes 13, such as just abutting or spacing.

[0044] Furthermore, in this embodiment, the middle portion of the connecting plate 2 is fixedly connected to the container body 1, further improving the stability of the connecting plate 2 and preventing the connecting plate 2 from being deformed (bent) by the resistance of the cell fluid, thereby causing wear due to the relative movement between the connecting plate 2 and the container body 1.

[0045] Furthermore, in this embodiment, the container body 1 is provided with a plurality of interfaces 121 and a dissolved oxygen sensor 5 and a pH sensor 6. Furthermore, the plurality of interfaces 121 are provided on the top film 12, and the interfaces 121 include a harvest interface, a liquid addition interface, a reagent interface, a sampling interface, an air intake interface, and an exhaust interface.

[0046] Furthermore, in this embodiment, a dissolved oxygen sensor 5 and a pH sensor 6 are respectively provided on the bottom film 11 and the top film 12 , so as to measure the dissolved oxygen and pH value in the internal space 4 of the container body 1 .

[0047] Furthermore, if Figure 3 and Figure 4 As shown, in this embodiment, the connecting plate 2 is flexible, such as made of a flexible film material. When the container body 1 is inflated and propped up, the connecting plate 2 is propped up. After the container body 1 is deflated, it can be folded for easy storage and packaging. Of course, in other embodiments, the connecting plate 2 can also be made of a hard material. In addition to the whole structure, the connecting plate 2 can be made into a foldable structure for easy storage and packaging.

[0048] Furthermore, in this embodiment, one connecting plate 2 is provided and is located in the middle of the container body 1 .

[0049] Embodiment 2:

[0050] Figure 5 Another embodiment of the cell culture container of the present invention is shown. The structure of this embodiment is basically the same as that of the first embodiment, except that at least two connecting plates 2 are provided, which are arranged at intervals along the length direction of the container body 1. At least two connecting plates 2 are provided to improve the mixing effect of the cell suspension during the culture process.

[0051] Embodiment three:

[0052] Figure 6 and Figure 7Another embodiment of the cell culture container of the present invention is shown. The structure of this embodiment is basically the same as that of the second embodiment, and the only difference is that at least two connecting plates 2 are provided, which are arranged alternately along the length and width direction of the container body 1. In other words, part of the connecting plates 2 are arranged at intervals along the length direction of the container body 1, and the other part of the connecting plates 2 are arranged at intervals along the width direction of the container body 1. The container body 1 is loaded with a cell suspension and fixed on the swing tray of the equipment. When the tray is swung in the front-to-back direction (the length direction of the container body 1), the cell suspension can flow back and forth through the through holes 3 on the connecting plates 2 arranged at intervals along the length direction of the container body 1 to achieve mixing in one direction. When the tray is swung in the left-right direction (the width direction of the container body 1), the cell suspension can flow back and forth through the through holes 3 on the connecting plates 2 arranged at intervals along the width direction of the container body 1 to achieve mixing in the other direction, thereby achieving bidirectional mixing. The bidirectional mixing helps to more fully mix the cell suspension during the culture process.

[0053] Embodiment 4:

[0054] Another embodiment of the cell culture container of the present invention has a structure that is basically the same as that of the first embodiment, except that the container body 1 is a rigid structure, that is, it is made of a rigid material, which can also be called a hard material, so that the container body 1 has a fixed shape at room temperature and pressure, and the bottom surface of the container body 1 is a polygon, a circle, an ellipse or a waist shape. The advantage of the rigid container body 1 over the flexible one is that it is easier to manufacture and use. If it is made of stainless steel or other materials, it can be reused after sterilization.

[0055] Embodiment five:

[0056] Figure 8 An embodiment of the shaking table of the present invention is shown. The shaking table of this embodiment includes a movable platform 7, a platform drive and a cell culture container. The container body 1 is arranged on the movable platform 7. The platform drive can drive the movable platform 7 to swing around at least one axis within a certain angle range. For example, the movable platform 7 swings left and right or forward and backward around one axis, and swings left and right and forward and backward around two vertical axes. The shaking table includes all the technical features of the cell culture container and has all the advantages of the cell culture container.

[0057] In other embodiments, Fig. 9 As shown, the platform drive can drive the movable platform 7 to rotate.

[0058] Although the present invention has been disclosed as above with preferred embodiments, it is not intended to limit the present invention. Any technician familiar with the art can make many possible changes and modifications to the technical solution of the present invention by using the technical content disclosed above without departing from the scope of the technical solution of the present invention, or modify it into an equivalent embodiment of equivalent changes. Therefore, any simple modification, equivalent change and modification made to the above embodiments according to the technical essence of the present invention without departing from the content of the technical solution of the present invention should fall within the scope of protection of the technical solution of the present invention.

Claims

1. A cell culture container, comprising a container body (1), characterized in that: A connecting plate (2) is provided inside the container body (1), and a plurality of through holes (3) for cell fluid to pass through are distributed on the connecting plate (2).

2. The cell culture container according to claim 1, characterized in that: The container body (1) comprises a bottom film (11) and a top film (12), and the bottom film (11) and the top film (12) are combined to form a flexible container body (1).

3. The cell culture container according to claim 2, characterized in that: The connecting plate (2) is flexible or foldable.

4. The cell culture container according to claim 1, characterized in that: Both ends of the connecting plate (2) are provided with fixing parts which are fixedly connected to the container body (1).

5. The cell culture container according to claim 4, characterized in that: A fixing portion fixedly connected to the container body (1) is provided in the middle of the connecting plate (2).

6. The cell culture container according to claim 1, characterized in that: The container body (1) is provided with a plurality of interfaces (121) as well as a dissolved oxygen sensor (5) and a pH sensor (6).

7. The cell culture container according to claim 1, characterized in that: The container body (1) is a rigid structure.

8. The cell culture container according to claim 7, characterized in that: The bottom surface of the container body (1) is polygonal, circular, elliptical or waist-shaped.

9. The cell culture container according to any one of claims 1 to 8, characterized in that: The connecting plate (2) is provided and is located in the middle of the container body (1).

10. The cell culture container according to any one of claims 1 to 8, characterized in that: At least two connecting plates (2) are provided and are arranged at intervals along the length direction of the container body (1).

11. The cell culture container according to any one of claims 1 to 8, characterized in that: At least two connecting plates (2) are provided and are arranged alternately along the length and width direction of the container body (1).

12. A rocking table, characterized in that: The cell culture container comprises a movable platform (7), a platform drive and any one of claims 1 to 11, wherein the container body (1) is arranged on the movable platform, and the platform drive is used to drive the movable platform (7).

13. The rocking table according to claim 12, characterized in that: The platform drive can drive the movable platform (7) to swing around at least one axis within a certain angle range or drive the movable platform (7) to rotate.

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