Method for improving growth and drought-resistant ability of wheat by using arbuscular mycorrhizal fungi

A technology of arbuscular mycorrhizal fungi and mycorrhizal fungi, applied in the application field of agricultural ecological microorganisms, can solve the problems that drought resistance does not necessarily increase yield, cannot be accepted by the whole people, and shortage of fresh water resources, so as to improve the symbiotic relationship of infection, Improve drought resistance and enhance the effect of beneficial symbiosis

Active Publication Date: 2014-06-04
LANZHOU UNIVERSITY
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  • Claims
  • Application Information

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Problems solved by technology

The development of irrigation technology can effectively alleviate the threat of drought, but the shortage of fresh water resources is a worldwide problem; although transgenic technology can improve the drought resistance of crops to a certain extent, but the transfer of drought resistance genes to improve crop drought resistance does not necessarily increase yield In addition, this technology is still controversial and cannot be accepted by the whole people; and improving cultivation techniques, such as conservation tillage, ground covering and other drought-resistant farming methods, cannot fundamentally solve the problem

Method used

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  • Method for improving growth and drought-resistant ability of wheat by using arbuscular mycorrhizal fungi
  • Method for improving growth and drought-resistant ability of wheat by using arbuscular mycorrhizal fungi
  • Method for improving growth and drought-resistant ability of wheat by using arbuscular mycorrhizal fungi

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Glomus monosporum was used as the fungal inoculum, and a plastic pot with a diameter of 21 cm and a height of 16 cm was used as a culture container.

[0025] A. Experimental treatment

[0026] Hexaploid wheat cv. L8275 was used as host plant. Select plump, uniform and healthy wheat seeds, disinfect them with 0.1% sodium hypochlorite for 2 minutes, and then clean them with distilled water.

[0027] The mixture of vermiculite and nutrient soil (the volume ratio of vermiculite and nutrient soil is 1:1) is used as the cultivation substrate (the field water capacity of the substrate is 39.2%), and the vermiculite and nutrient soil are purchased from the market.

[0028] Before inoculation, the substrate used was sterilized by dry heat at 160°C for 4 hours, and then put into a culture container sterilized with pasteurized solution after cooling down naturally. The fungal inoculum of the above-mentioned fungal inoculum is tiled below the soil layer 5cm, and the inoculum of 1...

Embodiment 2

[0030] The experimental treatment method was the same as in Example 1, except that Acaulospora laevis was used as the mycorrhizal fungus inoculant.

[0031] Inoculation effect experiment

[0032] The water gradient control and time in the experiment refers to the water control treatment by weighing watering every day when the flag leaves appear, in which the full water supply is 80% of the field water capacity, and the drought treatment is 30% of the field water capacity.

[0033] After the sampling treatment specifically refers to the water, the experimental treatment is divided into two groups. One group takes samples to analyze the symbiotic relationship, growth and physiological and biochemical indicators; the other group resumes normal water supply until the wheat

[0034] Ripe harvest yield.

[0035] Control group: the difference from Example 1 is that when sowing, each pot is first filled with 3kg of sterilized substrate, and then 10g of sterilized fungal inoculum (to ...

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Abstract

The invention discloses a method for improving growth and drought-resistant ability of wheat by using arbuscular mycorrhizal fungi to bring the reciprocal symbiosis relation between AM fungi and host plants under drought stress into play reasonably and efficiently. According to the method, the AM fungi having a close symbiotic relation with the wheat are selectively used, and the symbiotic relation between the AM fungi and the wheat is built in a condition control mode to promote the wheat growth and improve drought adaptability. The AM fungi comprise one of glomus monosporum and acaulospora laevis, the two kinds of AM fungi are respectively propagated in an expanding mode in an expanding propagation matrix by using red clovers, and then two AM fungi inoculants and the wheat are cultured together in a symbiotic culture medium. The effective path for profitable symbiosis of the AM fungi and the host plants in adversity conditions is provided, the infection and symbiosis relation with the host plants of the AM fungi is improved in a man-made mode, and the yield of dry crops is improved.

Description

technical field [0001] The invention belongs to the application technology of agricultural ecological microorganisms, and specifically relates to a method for improving wheat yield and drought resistance by using arbuscular mycorrhizal fungi that have a close symbiotic relationship with plants to establish a mutually beneficial symbiotic relationship through co-cultivation. Background technique [0002] Wheat is one of the main food crops for human beings. 70% of the world's wheat planting area is distributed in arid and semi-arid regions, and drought has become the most important factor restricting its production. The development of irrigation technology can effectively alleviate the threat of drought, but the shortage of fresh water resources is a worldwide problem; although transgenic technology can improve the drought resistance of crops to a certain extent, but the transfer of drought resistance genes to improve crop drought resistance does not necessarily increase yield...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G7/06A01G7/00
Inventor 祝英熊友才陈应龙王兆滨王建永
Owner LANZHOU UNIVERSITY
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