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Method for carrying out industrialized production on arbuscular mycorrhizal fungi agent

An arbuscular mycorrhizal fungus, industrial technology, applied in the direction of seed immunity, can solve the problems of the small number of spore propagules, the limited number of inoculants produced, and the impact of environmental factors, and achieves low price, short production cycle, and promotion. Yield effect

Active Publication Date: 2013-09-25
黑龙江省庆东阳光农业生物科技股份有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The present invention aims to solve the problems of long production period, large influence by environmental factors, limited production quantity of bacterial agents, small number of spore propagules, unstable quality and planting management problems in the existing low-cost and large-scale methods for the production of arbuscular mycorrhizal fungal agents. cumbersome problem, and a kind of factory production method of arbuscular mycorrhizal fungal agent provided

Method used

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  • Method for carrying out industrialized production on arbuscular mycorrhizal fungi agent
  • Method for carrying out industrialized production on arbuscular mycorrhizal fungi agent
  • Method for carrying out industrialized production on arbuscular mycorrhizal fungi agent

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specific Embodiment approach 1

[0016] Specific embodiment one: the method for industrialized production of arbuscular mycorrhizal fungus inoculum in this embodiment is carried out according to the following steps:

[0017] 1. Mix soil, sand and vermiculite in a volume ratio of 5:2:3 to form a substrate, then dilute formalin containing 40% formaldehyde 50 times with water and spray evenly on the substrate, then seal it with plastic cloth 1 Day and night, then spread out and expose to the sun for 4 to 6 days to obtain the arbuscular mycorrhizal fungus culture medium;

[0018] 2. The seeds of sorghum, alfalfa or clover are scrubbed with warm water at 20-30°C for 2-3 times, then soaked in 0.3% potassium permanganate solution for 3-4 hours, then washed with clean water, and then rinsed with 20 Soak the seeds in clean water at ~30°C for 24 hours, wash them 2 to 3 times, accelerate germination, and complete the host seed treatment;

[0019] 3. Put the arbuscular mycorrhizal fungus culture medium into a culture ta...

specific Embodiment approach 2

[0031] Specific embodiment two: the difference between this embodiment and specific embodiment one is: in step one, every cubic meter of substrate is sprayed with 500mL formalin dilution containing formaldehyde. Other steps and parameters are the same as those in Embodiment 1.

specific Embodiment approach 3

[0032] Embodiment 3: The difference between this embodiment and Embodiment 1 or 2 is that in step 2, the length of the cultivation pool is 5 meters and the width is 2 meters. Other steps and parameters are the same as those in Embodiment 1 or 2.

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Abstract

The invention discloses a method for carrying out industrialized production on an arbuscular mycorrhizal fungi agent, and relates to a production method of arbuscular mycorrhizal fungi. The method comprises the following steps: 1. preparing and treating the arbuscular mycorrhizal fungi agent; 2. carrying out seed treatment on hosts; 3. carrying out breeding; and 4. managing. The method disclosed by the invention has the advantages that the implementation of production just needs eight weeks, the number of effective spores of arbuscular mycorrhizal fungi in each gram of the obtained arbuscular mycorrhizal fungi agent is 120-150, the production cycle is short, and the yield is high. The method disclosed by the invention can be applied to the field of agriculture, gardens, and flowers.

Description

technical field [0001] The invention relates to a production method of an arbuscular mycorrhizal fungal agent. Background technique [0002] Mycorrhiza is a common plant symbiosis phenomenon in nature, which is a symbiosis formed between mycorrhizal fungi in the soil and the roots of higher plants. Arbuscular mycorrhizal (Arbuscular mycorrhizal, AM) is the most common type of endomycorrhizal, which is a symbiotic association between arbuscular mycorrhizal fungi and plant roots in soil. Arbuscular mycorrhizal fungi are widely distributed in nature. It is known that about 90% of the flowering plants, ferns and bryophytes in the world can live symbiotically with arbuscular mycorrhizal fungi. [0003] Arbuscular mycorrhizal fungi play an important role in the balance of plant nutrient cycle and the effective use of water, which can promote plant growth, improve the survival rate of plant transplantation, and improve the stress resistance of plants. Moreover, AM fungi have many...

Claims

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Application Information

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IPC IPC(8): A01C1/08
Inventor 宋福强
Owner 黑龙江省庆东阳光农业生物科技股份有限公司
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