Application method of AM (Arhusclar Mycorrhiza) fungi in cultivation of tree peony
An application method and technology of mycorrhizal bacteria are applied in the application field of AM mycorrhizal bacteria in peony cultivation, which can solve the problems of large one-time investment and high cost
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[0013] The fungi of the genus Asepsis and Geocystis of the present invention are purchased from the Institute of Plant Nutrition and Resources, Beijing Academy of Agriculture and Forestry Sciences, and are used for the propagation of target strains and the production of inoculants. The mixture of forest humus and river sand with a volume ratio of 6:1 was used as the substrate, and Hoagland nutrient solution was used for watering.
[0014] 1. Disinfect the matrix and plugs. Mix the matrix evenly with 0.1% carbendazim solution into peat, cover it with plastic film for 1 day, then wash it with water several times, after drying, sterilize the matrix continuously at 132°C for 20 minutes in a high-temperature and high-pressure autoclave, and let it sit naturally. gas; after the plug plate is cleaned, soak it in 0.1% potassium permanganate solution for 30 minutes and then dry it for use.
[0015] 2. Inoculant preparation. With clover as the host plant, spread the substrate, AM bact...
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