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Osmanthus fragrans polyphenol extract product, and preparation method and uses thereof

An extract, sweet-scented osmanthus technology, applied in the field of plant polyphenol extracts, can solve the problems of pseudohermaphroditism, amplified androgen response, etc., and achieve the effects of high-efficiency extraction, controllable quality, and wide application prospects.

Active Publication Date: 2013-07-31
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] 5α-reductase (5α-reductase) is a membrane protease dependent on reducing coenzyme II (NADPH), which can catalyze the conversion of testosterone (T) into dihydrotestosterone (DHT). T and DHT act on the same androgen receptor, However, the catalytic activity of DHT is stronger and has a stronger affinity for the androgen receptor, so the catalytic transformation of 5α-reductase can amplify the androgen response, which may cause pseudohermaphroditism, acne, androgenetic alopecia, female Symptoms such as hirsutism are also inseparable from the occurrence of benign prostatic hyperplasia and even prostate cancer, so it is of great significance to find high-efficiency 5α-reductase inhibitors

Method used

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  • Osmanthus fragrans polyphenol extract product, and preparation method and uses thereof
  • Osmanthus fragrans polyphenol extract product, and preparation method and uses thereof
  • Osmanthus fragrans polyphenol extract product, and preparation method and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Add about 20-40 mesh sweet-scented osmanthus powder into the inner cup made of polytetrafluoroethylene material of the intelligent microwave digestion instrument. The extractant is 80% acetone (that is, the acetone aqueous solution with a volume concentration of 80%), and the ratio of solid to liquid is 1:15 (g / ml), control the pressure at 200KPa, 80W microwave treatment for 3min, filter, and repeat the extraction twice (that is, replace the sweet-scented osmanthus powder with the filter residue, repeat the above microwave treatment and filtration twice in turn), combine the filtrate obtained from the three extractions, The filtrate was concentrated by vacuum rotary evaporation at 45°C, and the concentrated solution was pre-frozen at -80°C and vacuum freeze-dried to constant weight to obtain the polyphenol extract of osmanthus fragrans with a yield of 15.1%.

[0062] Determination and analysis of the components of the extract, the results are as follows: figure 1 , 2 s...

Embodiment 2

[0064] Add about 20-40 mesh osmanthus powder into the inner cup made of polytetrafluoroethylene material of the intelligent microwave digestion instrument, use 80% acetone as the extractant, the ratio of solid to liquid is 1:25 (g / ml), and the control pressure is 200KPa, 80W Microwave treatment for 5 minutes, filter, and repeat the extraction twice (that is, replace the sweet-scented osmanthus powder with the filter residue, repeat the above microwave treatment and filtration twice in turn), combine the filtrates obtained from the three extractions, and concentrate the filtrate by vacuum rotary evaporation at 45°C. After pre-freezing at -80°C, vacuum freeze-dry to constant weight to obtain osmanthus polyphenols extract with a yield of 23.5%.

[0065] Composition determination and analysis were carried out on the extract, which contained chlorogenic acid, caffeic acid, p-coumaric acid and verbascoside. Colorimetric method and HPLC method were used to analyze the composition con...

Embodiment 3

[0067] Add about 20-40 mesh sweet-scented osmanthus powder into the inner cup made of polytetrafluoroethylene material of the intelligent microwave digestion instrument. The extractant is 80% acetone, the ratio of solid to liquid is 1:35 (g / ml), and the control pressure is 300KPa. Microwave at 80W for 8 minutes, filter, and repeat the extraction twice (that is, replace the osmanthus powder with the filter residue, repeat the above microwave treatment and filtration twice in turn), combine the filtrates obtained from the three extractions, and concentrate the filtrate by vacuum rotary evaporation at 45°C. After pre-freezing at -80°C, vacuum freeze-dry to constant weight to obtain osmanthus polyphenols extract with a yield of 25.1%.

[0068] Composition determination and analysis were carried out on the extract, which contained chlorogenic acid, caffeic acid, p-coumaric acid and verbascoside. Colorimetric method and HPLC method were used to analyze the composition content of osm...

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Abstract

The invention relates to a plant polyphenol extract product extracted from Osmanthus fragrans, and a preparation method and uses thereof, and concretely discloses an Osmanthus fragrans polyphenol extract product preparation method. The method comprises the following steps: 1, mixing Osmanthus fragrans with an aqueous solution of an extracting agent according to a solid / liquid ratio of 1g / 15-60ml, carrying out microwave extraction of the obtained mixture under a pressure of 200-500KPa and a power of 80-200W for 3-20min, and repeating the above extraction process; and 2, mixing obtained filtrates to obtain a filtrate mixture, and drying the filtrate mixture to obtain an Osmanthus fragrans polyphenol extract product. The Osmanthus fragrans polyphenol extract product can be used for preparing antitumor medicines or healthcare products, and can also be used for inhibiting the activities of 5alpha-reductase.

Description

technical field [0001] The invention relates to the field of deep processing and comprehensive utilization of agricultural and sideline products. More specifically, it relates to a plant polyphenol extract extracted from osmanthus fragrans and its preparation method and application. Background technique [0002] Osmanthus fragrans Lour. Also known as sweet-scented osmanthus, jiulixiang, and golden millet, it is one of the top ten traditional famous flowers in my country. It is not only a famous spice plant, but also an excellent landscaping tree species, with good ecological, social and economic benefits. . Osmanthus osmanthus has a long history of cultivation in my country, and it is wild in Sichuan, Yunnan, Zhejiang, Guangxi, Hubei and other provinces and regions; it is generally planted from the Huaihe River Basin to the south of the lower reaches of the Yellow River, and it is mostly potted in the north. There are about 40 species of sweet-scented osmanthus in the world...

Claims

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Application Information

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IPC IPC(8): A61K36/63A23L1/29A61P35/00A61P13/08A23L33/00
Inventor 陆柏益熊丽娜杨佳佳胡银洲蒋易蓉谌灿曦周菲张英
Owner ZHEJIANG UNIV
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