Fermentation preparation and application of Chaetomium globosum dextranase

A technology of Chaetomium globosa and fermentation medium, which is applied in the direction of fermentation, glycosylase, enzyme, etc. It can solve the problems of no commercial dextranase product, low fermentation yield of dextranase, and inability to meet the needs of industrial preparation.

Active Publication Date: 2019-03-12
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] But at present, the fermentation yield of dextranase is still relatively low, which cannot meet the ne

Method used

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  • Fermentation preparation and application of Chaetomium globosum dextranase
  • Fermentation preparation and application of Chaetomium globosum dextranase
  • Fermentation preparation and application of Chaetomium globosum dextranase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1: Screening and identification of strains producing dextranase

[0040] The soil samples were diluted with saline to 10 -5Spread PDA medium (potato 200g, glucose 20g, agar 15-20g), culture at 28°C for 5 days, obtain pure strains after preliminary screening, and re-screen the strains with blue dextran T2000 medium, and compare them through the transparent circle , to obtain a strain with higher enzyme production. According to electron microscope observation and 18S rDNA identification, the strain is Chaetomium globosa, and the nucleotide sequence of 18S rDNA is shown in SEQ ID NO.1. The photos of the colony characteristics of Chaetomium globosa are shown in figure 1 , SEM image see figure 2 .

Embodiment 2

[0041] Embodiment 2: Optimization of fermentation medium

[0042] Pick the mycelium of the bulbous hair shell and insert it into the seed medium, and use a rotary constant temperature shaker for cultivation, the rotation speed of the shaker is 220r / min, the cultivation temperature is 28°C, and cultivate for 60h to obtain the seed liquid; the composition of the seed medium For: glucose 5g / L, sodium chloride 0.5g / L, yeast powder 5g / L, dipotassium hydrogen phosphate 0.5g / L, magnesium sulfate 0.2g / L, after sterilization, add filter-sterilized kanamycin to The final concentration was 50 μg / mL.

[0043] The seed solution was inserted into a 250mL shake flask filled with 50mL fermentation medium at an inoculum size of 1%, and the fermentation temperature was 28°C.

[0044] Different types of dextran (dextran T20, T40, T2000) and different substances (glucose, maltose, lactose, soluble starch, corn dextrin, sucrose, fish meal peptone) were used as carbon sources; yeast extract, urea,...

Embodiment 3

[0046] Embodiment 3: the optimization of fermentation enzyme production condition

[0047] Pick the mycelium of the bulbous hair shell and insert it into the seed medium, and use a rotary constant temperature shaker for cultivation, the rotation speed of the shaker is 220r / min, the cultivation temperature is 28°C, and cultivate for 60h to obtain the seed liquid; the composition of the seed medium For: glucose 5g / L, sodium chloride 0.5g / L, yeast powder 5g / L, dipotassium hydrogen phosphate 0.5g / L, magnesium sulfate 0.2g / L, after sterilization, add filter-sterilized kanamycin to The final concentration was 50 μg / mL.

[0048] The seed solution was inserted into a 250mL shake flask with 50mL fermentation medium at an inoculation amount of 1%. The fermentation temperature was 28°C. The pH was set to 4.5, 5.0, 5.5, 6.0, 6.5, 7.0, 7.5, 8.0, 9.0, respectively. The inoculum amounts were set to 1%, 2%, 3%, 4%, 5%, and 6%, respectively. The filling volume is set to 20mL, 30mL, 40mL, 50...

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Abstract

The invention discloses fermentation preparation and application of Chaetomium globosum dextranase and belongs to the technical fields of fermentation technology, enzymic preparations and sugar engineering. A high-yield Chaetomium globosum dextranase fermentation method is determined by optimizing medium components and fermentation conditions through orthogonal test, thereby improving the yield ofChaetomium globosum dextranase up to 698.22 U/mL; when applied to hydrolyzing high-molecular-weight glucan and at a final concentration of 2 U/ml, the Chaetomium globosum dextranase can achieve a high-molecular-weight glucan (T2000) hydrolyzing rate up to 97.9% within 15 minutes; the rate of inhibition of the Chaetomium globosum dextranase on biological membranes formed by streptococcus mutans can reach 97.9%, and the clearance rate of the biological membranes reaches up to 49.07%. The prepared Chaetomium globosum dextranase has a good application prospect in food and sugar industries and thefield of medicine.

Description

technical field [0001] The invention relates to the fermentation preparation and application of Chaetomium globosa dextranase, which belongs to the fields of fermentation technology, enzyme preparation and industrial application. Background technique [0002] Enzyme preparations refer to substances with enzymatic properties extracted from organisms, which have been used in various fields such as medicine, chemical industry, food, brewing, etc., with a very wide range of applications. The food processing industry is closely related to people's lives. Enzymes are used more and more in the food processing industry, and their role is becoming more and more important. It is greatly reflected in meat processing, deep hydrolysis of protein and as food additives. [0003] Dextranase is a hydrolase that specifically cleaves α-1,6 glucosidic bonds in dextran molecules. From the perspective of the hydrolysis of dextran by the enzyme, the known dextranases are divided into two categori...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N9/46C12P19/14C12P19/00A23L29/00A61K35/74A61P1/02A61K8/99A61Q11/00C12R1/645
CPCA61K8/99A61K35/74A61Q11/00A23L29/065A61P1/02C12N1/14C12N9/2454C12P19/00C12P19/14C12Y302/01011C12N1/145C12R2001/645
Inventor 田亚平杨柳周楠迪
Owner JIANGNAN UNIV
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