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Antivariant streptococcus polypeptide and use thereof and preparation method

A technology of Streptococcus mutans and stimulant polypeptides, applied in the field of medicine and biology, can solve the problems of normal flora imbalance, high cost, and restrictions on the large-scale preparation of polypeptides, and achieve the effect of low cost

Inactive Publication Date: 2009-05-06
STOMATOLOGICAL HOSPITAL NO 4 ARMY SURGEON UNIV PLA
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Problems solved by technology

However, colicin has a broad-spectrum antibacterial effect against both cariogenic and non-cariogenic bacteria, and its simple application in the oral cavity can lead to an imbalance of normal flora in the oral biofilm
Moreover, the application of chemical synthesis to synthesize anti-Streptococcus mutans peptides has a high cost, which limits the large-scale preparation of peptides. Therefore, it is more important to use genetic engineering technology to prepare antimicrobial peptides.

Method used

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  • Antivariant streptococcus polypeptide and use thereof and preparation method
  • Antivariant streptococcus polypeptide and use thereof and preparation method
  • Antivariant streptococcus polypeptide and use thereof and preparation method

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Embodiment Construction

[0062] The present invention will be described in further detail below in conjunction with the accompanying drawings and embodiments. It should be understood that these examples are only used to illustrate the present invention and are not intended to limit the scope of the present invention.

[0063] The method for preparing the fusion protein of colicin Ia channel domain (Colicin Ia channel domain) and Streptococcus mutans sensory stimulation polypeptide CSP as described above is prepared according to the following steps:

[0064] 1) Cloning of Colicin Ia channel domain-CSP fusion gene

[0065] The host bacteria loaded with colicin Ia (gifted by Professor Qiu Xiaoqing, Transplantation Immunology Laboratory, West China Hospital, Sichuan University) was amplified to extract the plasmid; The points were mutated into EcoRI and BamHI respectively;

[0066] Primer 1 (32nt): 5'-CGGAATTCGACGCAATTAATTTCACAACAGAG-3'

[0067] Primer 2 (31nt): 5'-GCGGATCCAATACCCCCAGAACTTATTCGCTT-3' ...

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Abstract

The invention belongs to the biological medicine field, and in particular relates to anti-variation streptococcus polypeptide, application and a preparation method thereof. The method is characterized in that the gene clone technique is adopted to establish MBP, Colicin Ia channel domain and CSP fudion gene, and obtain high efficiency expression in the Ia. The invention also discloses a ribonucleotide sequence for encoding recombinant polypeptide, and recombinant plasmid. The antibiotic polypeptide uses cariogenic Streptococcus mutans to forms an ion channel directly on a cell membrane of a target cell to achieve the bactericidal aim.

Description

technical field [0001] The invention belongs to the field of medical biotechnology, and specifically relates to gene cloning, gene mutation, expression of foreign genes in prokaryotic cells, purification of target protein, colicin Ia channel domain (Colicin Ia channel domain) and Streptococcus mutans sensory stimulating polypeptide Preparation method and application of CSP fusion protein. Background technique [0002] Caries (dental caries) is a bacterial infectious disease with chronic progressive destruction of dental hard tissue under the influence of various factors in the internal and external environment of the oral cavity. one of the great diseases. In 1946, McClure and Hewitt confirmed that penicillin can inhibit the occurrence of dental caries in rats. The main mechanism is to inhibit the synthesis of bacterial cell walls, inhibit or interfere with the metabolism and synthesis of nucleic acid and protein pathways of bacteria to achieve the purpose of antibacterial....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K19/00C12N15/62C12N15/70A61K38/16A61P1/02
Inventor 倪龙兴高洁赵玮钦陈苏民
Owner STOMATOLOGICAL HOSPITAL NO 4 ARMY SURGEON UNIV PLA
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