Oral cavity cariogenic bacterium-resisting polypeptide Pm11 and preparation method thereof

A technology of antibacterial peptides and cariogenic bacteria, applied in the field of medicine and biology, can solve the problems of high synthesis cost and excessive length of natural antibacterial peptides

Inactive Publication Date: 2012-03-21
倪龙兴
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide an anti-oral cariogenic bacteria polypeptide Pm11 and its preparation method to overcome the problems of excessive length of natural antibacterial peptide and relatively high synthesis cost

Method used

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  • Oral cavity cariogenic bacterium-resisting polypeptide Pm11 and preparation method thereof
  • Oral cavity cariogenic bacterium-resisting polypeptide Pm11 and preparation method thereof
  • Oral cavity cariogenic bacterium-resisting polypeptide Pm11 and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0023] figure 1 It is the α-helical structure software prediction map of polypeptides Pm11 and Pm14.

[0024] figure 2 is an electron micrograph of peptide-treated bacteria. A, normal S. mutans; B, Pm11-treated S. mutans; C, Pm14-treated S. mutans; D, normal blood S. mutans; E, Pm11-treated S. mutans; F, Pm14-treated S. mutans Streptococcus mutans. It can be seen that the surface of the bacteria treated with the polypeptide changes, and the phenomenon of porous or bacterial disintegration and shrinkage appears.

[0025] 1. Design and synthesis of peptides

[0026] 1.1 Design of peptides

[0027] The polypeptide is designed by intercepting the active center of pleurocidin and deleting and replacing amino acids. The physical properties of the designed peptides are shown in Table 1. The parameters of the α-helical structure are shown in Table 1, and the schematic diagram is shown in figure 2 .

[0028]

[0029]

[0030] 1.2 Fmoc method to synthesize peptides.

[0...

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Abstract

The invention belongs to the technical field of medication biology, and relates to an Fmoc (Fluorenylmethyloxycarbonyl chloride) chemical synthetic polypeptide, in particular to an oral cavity cariogenic bacterium-resisting polypeptide Pm11 and a preparation method thereof. The preparation method comprises the following steps of: searching for the active center of a natural polypeptide pleurocidin which has certain antimicrobial and bactericidal capabilities for common oral cavity cariogenic bacteria; and on the basis of determining the active center, performing deletion and replacement of amino acids on the natural polypeptide pleurocidin to form a novel antibacterial polypeptide having the bactericidal capability, wherein the sequence of the novel antibacterial polypeptide Pm11 is WFKFFKKFFKKWK; and the common oral cavity cariogenic bacteria mentioned in the preparation method refer to streptococcus mutans, streptococcus sobrinus and streptococcus sanguis. By adopting the polypeptide Pm11, the problems of over large length and relatively high synthesis cost of a novel antibacterial peptide are solved.

Description

technical field [0001] The invention belongs to the technical field of medicine and biology, and relates to Fmoc chemically synthesized polypeptide, that is, a polypeptide Pm11 for resisting oral cariogenic bacteria and a preparation method thereof. The polypeptide has the ability of resisting oral cariogenic bacteria, especially bacteria of the genus Streptococcus. Background technique [0002] Although a lot of manpower and material resources have been invested, dental caries is still one of the three major non-communicable diseases in the world. Caries is a chronic and progressive bacterial infectious disease that occurs in dental hard tissues under the influence of various factors in the internal and external environment of the oral cavity. Streptococcus mutans and Streptococcus farus are the main cariogenic bacteria. In 1946, McClure and Hewitt confirmed that penicillin can inhibit the occurrence of dental caries in rats. The main mechanism is to inhibit the synthesis ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/08A61P1/02A61P31/04
Inventor 倪龙兴陶睿
Owner 倪龙兴
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