Thermophilic bacillus DF7 strain and application thereof

A technology of Bacillus thermophilus and strain, applied in the field of Bacillus thermophilus DF7 strain to achieve the effect of neat edges

Active Publication Date: 2019-02-15
HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there are many studies on S-TE technology for the hydrolysis of excess sludge, but there are few reports on the use of antibiotic slag for wall-breaking and cell lysis. This is mainly because the existing treatment and disposal technologies for antibiotic slag are mainly incineration, fertilization, Feed, landfill and energy, etc., the pretreatment technology is mostly physical, chemical and other biological treatment methods

Method used

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  • Thermophilic bacillus DF7 strain and application thereof
  • Thermophilic bacillus DF7 strain and application thereof
  • Thermophilic bacillus DF7 strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Isolation and screening of thermophilic bacillus DF7

[0037] The separation and screening method of thermophilic bacillus DF7 of the present invention is to adopt gradient dilution separation method to obtain, and its method comprises the following steps:

[0038] Sample collection: Samples were taken from the center of the compost matrix after mixed aerobic composting of reed straw and activated sludge according to the volume ratio V / V=2:1.

[0039] Sample pretreatment: Take the above samples and put them into a sterilized container filled with sterile water, seal and shake evenly for 10 minutes, let the suspension stand, take the supernatant and centrifuge, take the upper layer of bacteria as the bacteria suspension, and set aside.

[0040] Enrichment culture: Inoculate 2 mL of the bacterial suspension into 200 mL of liquid medium, place in a constant temperature incubator at 60°C for 48 hours, and obtain culture medium for expanding strains.

[0041] Grad...

Embodiment 2

[0046] Example 2 Identification of physicochemical properties of thermophilic bacillus DF7

[0047] The identification method of thermophilic bacillus DF7 of the present invention comprises the following steps:

[0048] (1) Observe the colony shape and strain shape of the thermophilic strain DF7. The thermophilic bacteria of the present invention were cultured at 60°C for 12 hours. figure 1 shown.

[0049] The thermophilic bacterial strain DF7 of the present invention belongs to the genus Thermobacillus and is Gram positive. figure 2 shown.

[0050] (2) Physiological and biochemical characteristics of the strain

[0051] .

[0052] (3) DNA sequence information and phylogenetic analysis

[0053] MEGA7.0 software was used to display the 16S rDNA sequence phylogenetic tree of the thermophilic strain DF7 and related strains by the adjacent connection method, and the similarity calculation was repeated 1000 times. image 3 The middle growth tree node only displays values ​​...

Embodiment 3

[0054] Example 3 Lysis test of thermophilic bacillus DF7

[0055] using plateau growing E. coli as the model strain for the experiment. E. coli The culture solution was collected by centrifugation at 5000rpm for 10min and resuspended. 50% (V / V) of thermophilic bacteria (Abs 600 =1.0~1.5) Thermophilic Bacillus ( Bacillus Thermocopriae ) DF7 supernatant was added to the lysis system. Finally, 100mL of mixed bacterial solution was added to a 250mL Erlenmeyer flask and treated at 60°C for 16h, the results were as follows: Figure 4 shown.

[0056] Depend on Figure 4 It can be seen that with the increase of time, E. coli The cell lysis rate continued to increase and reached a maximum of 67% at 14 hours. It shows that the hydrolase secreted by thermophile DF7 will E. coli The cells are lysed, the intracellular organic matter is released, and the content of soluble organic matter in the supernatant increases.

[0057] It can be seen that the protease and amylase secrete...

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Abstract

The invention relates to a thermophilic bacillus DF7 strain. The thermophilic bacillus DF7 strain is preserved in the China General Microbiological Culture Collection Center, with a preservation number of CGMCC No.16459. The strain provided by the invention can efficiently hydrolyze antibiotic bacterial residues under the condition of aerobic fermentation, dissolve out intracellular organic substances, promote subsequent resource utilization, and promote the lysis of E.coli cells.

Description

technical field [0001] The invention relates to a thermophilic bacillus DF7 strain and application thereof. Background technique [0002] my country has become the world's largest producer of antibiotics, and a large amount of antibiotic residues will be produced during the production of antibiotics. According to the "National Hazardous Waste List" revised in 2008, antibiotic residues belong to culture medium waste in the production process of chemical raw materials and must be managed as hazardous waste. Its use as animal feed or feed additives and the production of organic fertilizers are all prohibited. It is expressly prohibited. In addition, antibiotic residues are rich in organic matter. The content of protein (about 30%~40% of dry basis) and other organic matter in antibiotic residues (dry basis) is as high as 90%, which has high biomass energy and can be directly incinerated Disposal is a great waste of resources. Therefore, if the organic substances (such as prot...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/06C12R1/01
CPCC12N1/06C12N1/20
Inventor 钟为章孟文茹马彩云刘中超邬甜媛孙熠李再兴
Owner HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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