Novel bacillus megaterium and application thereof

A technology of Bacillus megaterium and strains, applied in the field of microorganisms, can solve the problems of low organic matter content of bio-organic fertilizers, weak organic matter degradation ability, weak hydrolysis ability, etc., and achieves high nutrient content, rich enzyme system and low cost. Effect

Active Publication Date: 2016-05-04
SICHUAN NORMAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] At present, in the production of bio-organic fertilizers, Bacillus megaterium is only one of the mixed fermentation flora, and is often used together with Bacillus subtilis and other microorganisms. There is no report on using Bacillus megaterium alone to ferment bio-organic fertilizers
[0006] In addition, the patent with the notification number CN103468613B discloses a Bacillus megaterium JSSW-JD-2F5, whose protease hydrolysis ability (Up) is 8.59±1.54, and the hydrolysis ability is not strong, indicating that its ability to degrade organic substances is weak, and the production The content of organic matter in the bio-organic fertilizer is low and the quality is not good

Method used

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  • Novel bacillus megaterium and application thereof
  • Novel bacillus megaterium and application thereof
  • Novel bacillus megaterium and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1 Isolation and identification of Bacillus megaterium JD4 (Bacillus megaterium JD4) of the present invention

[0042] 1. Identification method

[0043] The strain of the present invention was isolated from rotten wheat straw in the farmland of Santai County, Mianyang City, and the following morphological and molecular biology identifications were carried out.

[0044] (1) Strain morphology and physiological and biochemical characteristics

[0045] The morphology, physiological and biochemical characteristics of the strains were identified according to conventional methods with reference to relevant literature such as "Berger's Manual of Identification of Bacteriology" (9th Edition) (Holt et al, 1994) and "Handbook of Common Bacterial System Identification" (Dong Xiuzhu et al., 2001).

[0046] (2) Molecular biological characteristics

[0047] The colony PCR technique was used to amplify the 16SrDNA gene sequence of the strain, and the specific operation was ...

Embodiment 2

[0086] Example 2 Detection of enzyme-producing properties of Bacillus megaterium JD4 (Bacillus megaterium JD4) of the present invention

[0087] 1. Experimental method

[0088] (1) Strain activation: aseptically open the strain tube, perform aseptic operation, inoculate the strain of Bacillus megaterium JD4 (Bacillus megaterium JD4) of the present invention on the beef extract peptone agar medium, culture at 37°C for 24 hours, and set aside;

[0089] (2) Determination of enzyme production characteristics: use an inoculation needle to take a small amount of the bacteria in the above step (1) and spot them on the casein medium plate and the starch medium plate respectively, and repeat the spotting for 3 times on each medium, and cultivate at 37°C 48h.

[0090] Among them, the composition of casein medium: Na2HPO4 1.07g / L, KH2PO40.36g / L, casein 4g / L, distilled water 1000mL, agar powder 20g / L, sterilized at 121°C for 20min.

[0091] Composition of starch medium: peptone 10.0g / L,...

Embodiment 3

[0101] The preparation of embodiment 3 organic fertilizer additives of the present invention

[0102] 1. Preparation of fermentation broth

[0103] 1. Strain activation: Aseptically open the strain tube, perform aseptic operation, inoculate the strain of Bacillus megaterium JD4 (Bacillus megaterium JD4) of the present invention on the beef extract peptone agar medium, cultivate at 28-37°C for 24-48h Finally, seal with parafilm and store at 4°C for later use;

[0104] 2. Preparation of seed bacterial suspension: inoculate the activated strain into sterilized beef extract peptone liquid medium, culture at 28-37°C, 150-170rpm for 24-48 hours, seal with a parafilm and store at 4°C. spare;

[0105] 3. Screen the most suitable fermentation medium:

[0106]Medium A: glucose 4.0-5.0g / L, corn powder 14.0-15.0g / L, sodium chloride 4.5-5.0g / L, pH 6.8-7.2;

[0107] Medium B: glucose 20.0-25.0g / L, corn powder 70.0-75.0g / L, sodium chloride 4.5-5.0g / L, pH 6.8-7.2;

[0108] Medium C: 40.0...

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Abstract

The invention discloses a bacillus megaterium which is a bacillus megaterium JD4 collected by the China Center for Type Culture Collection (CCTCC) and has a collection number of CCTCC NO: M2016033. The invention also discloses an application of the bacillus megaterium, and an organic fertilizer additive and a biological organic fertilizer prepared by using the bacillus megaterium for fermentation. The bacillus megaterium disclosed by the invention has good protease producing activity, can efficiently degrade organic substances, can be used for preparing biological organic fertilizers, and has good application prospects.

Description

technical field [0001] The invention relates to a new strain of bacillus megaterium and its application, belonging to the technical field of microorganisms. Background technique [0002] As our economy develops, industrial and agricultural wastes put enormous pressure on the environment. According to statistics, the country produces about 700 million tons of straw, 4 billion tons of animal husbandry waste, 20 million tons of urban sludge, and about 200 million tons of urban domestic waste every year. Making waste into bio-organic fertilizer can effectively solve the problem of waste pollution, and is of great significance to environmental protection and cost saving and efficiency increase. [0003] In the process of bio-organic fertilizer preparation, adding functional bacteria or fungal agent based on functional bacteria can speed up the process of organic fertilizer preparation, promote decomposing, adjust the content of various nutrients in organic fertilizer, and improv...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K10/18C02F3/34C05G1/00C05F11/08C05F17/00C12R1/11
CPCC02F3/34C05F3/00C05F11/08C05F17/00C12N1/20C12N1/205C12R2001/11C05F5/00C05F11/00Y02W30/40
Inventor 何兵赖金龙陶宗娅付倩韩娜李维
Owner SICHUAN NORMAL UNIVERSITY
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