Method for producing ethanol or acetone and butanol by taking lignocellulose as raw material

A technology of lignocellulose and acetone butanol, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of long enzymatic hydrolysis time, high investment cost, long production cycle, etc., and achieve high hydrolysis efficiency , the effect of small energy consumption

Active Publication Date: 2012-01-04
SHANDONG UNIV +1
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Problems solved by technology

[0004] Patent CN200810049574 (Publication No. CN101381743A) "Method for Producing Hydrogen and Ethanol from Straw Biomass Through Solid State Enzymolysis Pretreatment" analyzed the advantages and disadvantages of a series of such patents, while the CN200810049574 patent used grinding, enzymolysis, fermentation and other pro

Method used

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  • Method for producing ethanol or acetone and butanol by taking lignocellulose as raw material
  • Method for producing ethanol or acetone and butanol by taking lignocellulose as raw material
  • Method for producing ethanol or acetone and butanol by taking lignocellulose as raw material

Examples

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Example Embodiment

[0035] Example 1:

[0036] The corncob was pulverized into 1mm powder with a pulverizer, then rinsed with water, and then dried at 60°C overnight. Mix 500g of corncob flour below 1-2mm with 10L of water, and place it for anaerobic fermentation at 10°C for 1 day. When the pH drops to 4.5, adjust the wet disc grinder - Japan MKZA10 ultra-fine particle melter (Yoshiko). Sangyo Co., Ltd., Japan) The gap between the two upper and lower disc grinding discs should be 1 micron to a suitable gap. Mix the material and liquid refrigerated at 4 °C, add it to the ultra-fine melter, and grind for 10 to 15 circles, and its particle size is 2 μm. In between, the soluble sugar content was 0.385 g / g corncob, of which the glucose content was about 65% and the xylose content was about 20%. The complex cellulase was added to the pretreatment solution in an amount of 5%. The hydrolysis conditions were as follows: 10.0 mL pH 4.5, under the condition of 50 °C, 200 rpm rotary shaking for 48 h. The ...

Example Embodiment

[0037] Example 2:

[0038] The corncob was pulverized into a 2mm powder with a pulverizer. Mix 500g of corncob flour below 2mm with 5L of water, put it at 4°C for 1 day, and place it at 4°C for 2 days for anaerobic fermentation. When the pH drops to 5.0, adjust the wet disc grinder - Japan MKZA10 ultra-fine melting Crusher (Mashiko Sangyo Co., Ltd., Japan) The gap between the two upper and lower disc grinding discs is 2 microns to a suitable gap, and after grinding for 15 circles, add 10FPU / g Acremonium complex cellulase and 0.4% β- Xylosidase; pre-hydrolyze at 50°C for 1h after mixing, add 0.1% yeast powder, keep the temperature at 37°C after mixing, insert acetone-butanol-producing microorganism Clostridium beijerinckii ATCC55025 at an inoculum of 5%, and ferment 72h, the butanol content was 7.8g / L, and the acetone content was 4.1g / L.

Example Embodiment

[0039] Example 3:

[0040] Pulverize the bran into 1mm powder with a pulverizer, mix 500g of the material liquid with 5L of water, and place it for anaerobic fermentation at 4°C for 2 days. When the pH drops to 4.8, adjust the wet disc grinder - Japan MKZA10 Ultra The gap between the two upper and lower disc grinding discs of the microparticle thawing machine (Mashiko Sangyo Co., Ltd., Japan) is to a suitable gap of 10 microns, and the anaerobic material and liquid at 4 °C are mixed. , the soluble sugar content is 0.151g / g bran. The pretreatment solution was added with 6.4 FPU / g Acremonella cellulase (complex enzyme with endonuclease and exonuclease) and 40 μL / g β-xylosidase according to the addition amount of 5% for hydrolysis for 48h. The soluble sugar content was 0.531 g / g bran. The above-mentioned bran is pretreated and enzymatically hydrolyzed into the mixed liquid, adding 0.1% yeast powder, pH 5.5 to 7.0, and inserting acetone butanol to produce microorganism Clostridi...

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Abstract

The invention relates to a method for producing ethanol or acetone and butanol by taking lignocellulose as a raw material. According to the invention, a wet disc grinding machine-ultrafine grain melting and crushing machine physical method is adopted for pretreating the lignocellulose raw material, so that a cellulose structure wrapped by hemicellulose is opened and a cellulose crystalline structure is simultaneously opened, the method can be used for replacing all acid, alkali and gas explosion treatment methods, the conditions of high temperature and high pressure are not required, no external metal or organic matter such as sulfur is added, the pollution is avoided and the hydrolysis efficiency is high. By adopting the method, no secondary substance inhibiting the growth and the fermentation of yeast, bacteria and anaerobic bacteria is generated, and cellulose, the hemicellulose and lignin are not damaged.

Description

technical field [0001] The invention relates to a method for producing ethanol or acetone butanol by using lignocellulose as a raw material. Background technique [0002] At present, lignocellulosic biomass, such as straw, grain, reed, wood and other raw materials, is used for renewable energy development. One of the key problems is that cellulose and hemicellulose cannot be rapidly degraded or utilized by microorganisms, even if fiber is added. Vegetase and / or hemicellulase are used for hydrolysis, but their hydrolysis effect is also quite poor. In the past, cellulosic biomass raw materials were pretreated by physical, chemical or a combination of the two methods. In China, corn cob and straw are rich in cellulose and hemicellulose materials. As a plant variety of lignocellulosic biomass, the output of straw materials can reach 650 million tons, of which the annual output of corn cob can reach 40 million tons. As an important raw material for cellulosic biomass conversion...

Claims

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Application Information

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IPC IPC(8): C12P7/10C12P7/16C12P7/36C12P19/14C12R1/01C12R1/145C12R1/21C12R1/645
CPCY02E50/16Y02E50/10
Inventor 方诩程少博张杰夏蕊蕊肖林曲音波王明钰
Owner SHANDONG UNIV
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