Trichoderma koningiopsis strain and application of trichoderma koningiopsis to preparation of cellulase

A technology of cellulase and Trichoderma, applied in the direction of enzymes, enzymes, fungi, etc., can solve the problems of inability to hydrolyze and low activity level of β-glucosidase, and achieve the effect of reducing costs

Active Publication Date: 2012-11-21
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the β-glucosidase activity level of Trichoderma reesei strain is very low and cannot rapidly and completely hydrolyze all cellobiose produced by hydrolysis of cellulose into glucose

Method used

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  • Trichoderma koningiopsis strain and application of trichoderma koningiopsis to preparation of cellulase
  • Trichoderma koningiopsis strain and application of trichoderma koningiopsis to preparation of cellulase
  • Trichoderma koningiopsis strain and application of trichoderma koningiopsis to preparation of cellulase

Examples

Experimental program
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Effect test

Embodiment 1

[0050] Example 1. Isolation and identification of Trichoderma strain FCD3-1

[0051] 1. Obtaining strains

[0052] 1. Collection of soil samples

[0053] It was collected near a starch factory in Fangchenggang City, Guangxi, China in March 2009; the surface cover soil was removed, and 50 grams of soil about 5-20 cm below the surface was taken as a sample; a total of 13 samples were collected scattered.

[0054] 2. Isolation and screening of cellulase-producing strains

[0055] (1) Prepare separation medium with deionized water; each liter of separation medium contains: KH 2 PO 4 2.0g, (NH 4 ) 2 SO 4 1.4g, urea 0.3g, MgSO 4 ·7H 2 O 0.3g, CaCl 2 0.3g, FeSO 4 ·7H 2 O 5.0mg, MnSO 4 ·H 2 O 1.56mg, ZnSO 4 ·7H 2 O 1.4mg, CoCl 2 2.0mg, Avicel (Sigma PH101) 10g, agar 15g; pH5.0; sterilized by moist heat at 121°C for 20min; after sterilization, the plate is poured when cooled to 50°C.

[0056] (2) Take 10g of soil sample into a 250ml Erlenmeyer flask, add 90ml of sterile water and add appropri...

Embodiment 2

[0066] Example 2: Preparation of cellulase using Trichoderma strain FCD3-1

[0067] 1. Obtaining fermentation broth

[0068] 1. Prepare basic medium (liquid) with pH 5.0

[0069] 2. Preparation of spore liquid

[0070] (1) Sterilize the PDA medium (solid) at 121°C for 20 minutes.

[0071] (2) The spores of Trichoderma FCD3-1 obtained in Example 1 were washed with sterile water and made into a spore suspension, the spore concentration was 1×10. 10 Pcs / ml.

[0072] 3. Obtaining fermentation broth

[0073] (1) Take 100ml of basic medium and put it in a 500ml Erlenmeyer flask.

[0074] (2) Take the spore liquid of Trichoderma FCD3-1 and inoculate it into the minimal medium at a rate of 1% (volume percentage), culture at 28°C and 180 rpm for 5 days, and collect the culture.

[0075] (3) Centrifuge (4°C, centrifugal radius 7cm, 12000rpm, centrifugation for 5min) to remove the bacteria, and collect the supernatant to obtain the Trichoderma FCD3-1 fermentation broth.

[0076] 2. Cellulase enzymatic ...

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Abstract

The invention discloses a trichoderma koningiopsis strain and application of the trichoderma koningiopsis to the preparation of cellulase. The invention provides the trichoderma koningiopsis strain FCD3-1 capable of being used for preparing the cellulase, and the preservation number of the trichoderma koningiopsis strain FCD3-1 is CGMCC (China General Microbiological Culture Collection Center) No. 6050. The invention also provides a method for preparing the cellulase. The method comprises the following steps that the trichoderma koningiopsis strain FCD3-1 is subjected to liquid fermentation, fermentation products are collected, thalli are removed through centrifugation, obtained fermentation supernatant can be used as a cellulase preparation, the cellulase preparation can be used for efficiently hydrolyzing and crystallizing the cellulose, in addition, the final hydrolysis product is glucose, the cellulase preparation contains high-activity beta-glucosidase, the most proper pH value of the beta-glucosidase is 4.5, the most proper temperature is 60 DEG C, and in addition, better pH tolerance and temperature stability are realized. The cellulase preparation can be used for the lignocellulose hydrolysis.

Description

Technical field [0001] The invention relates to the field of biotechnology, in particular to a Trichoderma strain and its application in the preparation of cellulase. Background technique [0002] Plant biomass is the largest renewable biomass resource on the planet, and it is believed to play an important role like oil in the future. Cellulose and hemicellulose are the main components of plant biomass. Through the hydrolysis of cellulose by cellulase, cellulose can be converted into glucose, which can be further converted into ethanol or other useful chemicals (Lynd LR, Weimer PJ, van Zyl WH, et al. Microbial cellulose utilization: fundamentals and biotechnology. Microbiol Mol Biol Rev. 2002, 66: 506–577). [0003] The first generation of fuel ethanol was mainly produced by fermentation of sucrose or corn starch or other grains. In the past 30 years, Brazil, the United States and some other countries have used fuel ethanol as a mixed component of motor gasoline or as a fuel comp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N9/42C12P7/10C12P19/14C12P19/02C12R1/885
CPCY02E50/16Y02E50/10
Inventor 冯家勋张政刘君梁蓝健益马庆生
Owner GUANGXI UNIV
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