Method for cultivating phaffiafhodozyma using astaxanthin synthesis accelerant

The technology of Phaffia rhodozyma and astaxanthin is applied in the directions of fermentation, fungi, etc., which can solve the problems of high production cost and low yield of astaxanthin, and achieve the advantages of low cost of raw materials, high promotion and utilization value, and increased yield. Effect

Inactive Publication Date: 2008-01-16
DALIAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the existing research and production practice of using Phaffia rhodozyme microbial fermentation to produce natural astaxanthin, the yield of astaxanthin per unit weight of cells is not high, and the production cost is relatively high

Method used

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  • Method for cultivating phaffiafhodozyma using astaxanthin synthesis accelerant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] A, the selection of raw materials: select the bright orange that contains the synthetic precursor of Phaffia rhodozyma astaxanthin to peel and weigh;

[0018] B. Juicing: Juicing fresh oranges to obtain a solid-liquid mixture;

[0019] C. Preparation of accelerator additive liquid: filter the solid-liquid mixture squeezed out of the juice, sterilize the filtrate at 115°C for 20 minutes, then aseptically filter to remove the precipitate, measure the reducing sugar content, and store it at 4°C for later use;

[0020] D. Liquid seed culture: activate the slant-preserved strains on 10Bx wort slant medium at 22°C, and culture them in YM medium at 22°C with liquid ventilation;

[0021] E. Fermentation of Phaffia rhodozyme: Add the liquid seeds cultivated in step d with 8% of the total volume of the YM medium, and cultivate Phaffia rhodozyme at 22° C. with liquid ventilation for 24 hours;

[0022] F. Liquid aeration culture: Add the accelerator additive solution prepared in s...

Embodiment 2

[0026] Get banana and implement by A, B, C, D steps in embodiment 1;

[0027] E, Phaffia rhodozyme fermentation: After adding the liquid seeds cultivated in step d with 12% of the total volume of the YM medium, directly add the accelerator additive solution prepared in step C;

[0028] F. Liquid ventilation culture: Add the accelerator additive solution prepared in step C to 4% of the total volume of the fermented liquid in the cultured Phaffia rhodozyme, continue to ferment for 96 hours, and collect yeast cells.

[0029] G. Extract astaxanthin: measure astaxanthin content in yeast cells, then extract astaxanthin according to conventional methods, and then extract bacterial protein.

[0030] The obtained cell yield, astaxanthin yield and cell astaxanthin content can be increased by 54.4%, 65.5% and 7.2% respectively compared with the control.

Embodiment 3

[0032] A. Selection of raw materials: select fresh spinach containing the synthetic precursor of Phaffia rhodozyma astaxanthin, remove the roots and yellow leaves, wash and dry, then weigh;

[0033] B. Juicing: Take 700 grams of spinach and add 300 milliliters of water to squeeze the juice to obtain a solid-liquid mixture;

[0034] C. Preparation of accelerator additive liquid: filter the solid-liquid mixture from the squeezed juice, sterilize the filtrate at 110°C for 20 minutes, and filter aseptically to remove the precipitate to obtain light green spinach juice, measure the reducing sugar content, store at 4°C for later use ;

[0035] D. Liquid seed culture: activate the slant-preserved strains on 10Bx wort slant medium at 25°C, and culture them in YM medium at 25°C with liquid ventilation;

[0036] E. Fermentation of Phaffia rhodozyme: Add the liquid seeds cultivated in step d with 6% of the total volume of the YM medium, and culture Phaffia rhodozyme at 25° C. with liqui...

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Abstract

A method for culturing red-hair yeast with shrimpanin synthesis accelerator. Use shrimpanin synthesis former-rich fruits, vegetables and tea juice such as fresh orange, spinach, tea leaf, etc as materias, and prepare shrimpanin synthesis accelerator by pressing juice or immersion. Add the shrimpanin synthesis accelerator in red-hair yeast medium at different fermention time; ferment for some time; collect yeast cell. Also we can furtherly do cell wall breaking treatment and extrat shrimpanin from the cell with acetone. The red-hair yeast achieved has high shrimpanin content, and the shrimpanin content can be advanced largely just by adding some shrimpanin synthesis accelerator. It has the advantages of investment little, raw materials cost little, and so on. Both the achieved red-hair yeast and the shrimpanin extracted from it can be used as feedingstuff additive for top-grage aquatic products and birds, and can make the culturing products have high commondity value.

Description

technical field [0001] The invention relates to the field of preparation of biologically active substances, in particular to a method for preparing natural astaxanthin by yeast fermentation. Background technique [0002] Astaxanthin is a ketone carotenoid with high economic value, which is bright red. It widely exists in the biological world, especially in aquatic animals such as shrimp, crab, fish and bird feathers. Because astaxanthin has a unique coloring function and strong antioxidant properties, it is far stronger than other carotenoids in biological functions such as anti-cancer, enhancing immunity and anti-photosensitivity, so it is widely used in feed, food, cosmetics and medicine, etc. The field has broad application prospects. According to its source, astaxanthin can be divided into chemical synthesis and natural two categories. At present, chemically synthesized astaxanthin is mainly used as an animal feed additive, such as some ornamental and farmed fish, cru...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16C12P23/00
Inventor 孙玉梅华艳艳
Owner DALIAN POLYTECHNIC UNIVERSITY
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