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Method for producing 1, 3-propanediol through fermentation

A technology of propylene glycol and fermentation liquid, applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., to achieve the effects of reducing fermentation production costs, increasing concentration, and simple operation

Active Publication Date: 2013-06-26
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the current research, there is no report that exogenous addition of malic acid promotes the synthesis of 1,3-propanediol by microorganisms

Method used

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  • Method for producing 1, 3-propanediol through fermentation
  • Method for producing 1, 3-propanediol through fermentation
  • Method for producing 1, 3-propanediol through fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] 1) Bacterial species: Klebsiell apneumoniae CGMCC1.9131.

[0032] (2) culture medium

[0033] LB medium (g·L -1 ): Yeast powder 5g / L, peptone 10g / L, NaCl 10g / L, agar 10g / L, adjusted to pH 7.0. For short-term preservation and activation of Klebsiella species.

[0034] The seeds and fermentation medium are shown in Table 1:

[0035] Table 1 medium composition table (L -1 )

[0036]

[0037] Wherein, the formula of trace element solution is shown in the following table.

[0038] Table 2 Trace element solution formula (L -1 )

[0039]

[0040] (3) Training method:

[0041] A. Seed activation

[0042] The strains preserved from glycerol tubes were inoculated into LB medium slant for activation, and incubated at 37°C for 12 hours to activate the seeds.

[0043] B. Seed cultivation

[0044] Seed culture: 250mL triangular flask sealed with 9 layers of gauze, filled with 100mL of liquid, connected to a ring of slant lawn, aerobic seed culture in a shaker, temper...

Embodiment 2

[0061] (1) strain: with embodiment 1.

[0062] (2) The seed culture medium is the same as in Example 1, and the fermentation medium is the same as in Example 1 except that the concentration of glycerol is 15g / l.

[0063] (3) Training method:

[0064] A. Seed activation

[0065] The strains preserved from glycerol tubes were inoculated into LB medium slant for activation, and incubated at 37°C for 12 hours to activate the seeds.

[0066] B. Seed cultivation

[0067] Seed culture: 250mL triangular flask sealed with 9 layers of gauze, filled with 50mL of liquid, connected to a ring of slant lawn, and carried out aerobic seed cultivation in a shaker at a temperature of 30°C and a speed of 150r min -1 .

[0068] C. Fermentation culture

[0069] When carried out in a 5L stirred fermenter, the liquid volume is 4L, the inoculum size is 1%, and 0.5vvm air is introduced to carry out the microaerobic fermentation culture, and the stirring speed is 250rpm. Malic acid was added to th...

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Abstract

The invention belongs to the technical field of biochemical engineering, and discloses a method for producing 1,3-propanediol through fermentation, which comprises the following steps of: preparing a culture medium; culturing seeds; and fermenting to obtain the 1,3-propanediol. By adding malic acid into a fermentation medium, the final concentration is 0.1-10.0g / L; and the adding time is selectedfrom an initial stage of fermentation to an exponential phase of strains. The added malic acid can promote tricarboxylic acid circulation of thalli, and provide more reducing coenzymes and energy. The method is suitable for the aerobic fermentation process of the 1,3-propanediol. The method has the advantages that: producing strains can be promoted to utilize a substrate of glycerol, the concentration and production strength of the 1,3-propanediol are obviously improved, and the production cost is reduced.

Description

technical field [0001] The invention belongs to the technical field of biochemical industry, and in particular provides a method for promoting the synthesis of 1,3-propanediol by microorganisms by adding malic acid from an external source. Background technique [0002] 1,3-Propanediol (PDO) is an important chemical raw material, which can be used as an organic solvent in high-pressure lubricants, dyes, inks, antifreeze and other industries. The use of PDO in ink can improve the adsorption and stability of ink. PDO can be used to synthesize heterocycles, drug intermediates, polyesters and polyurethanes, especially as a monomer for polyester PTT. Potential uses for PDO also include plasticizers for polymers used in the manufacture of thermoplastic polyurethane (TPU) and polyvinyl chloride (PVC). As a diol, it can replace intermediates such as 1,4-butanediol and neopentyl glycol. Since PDO also has diol functional groups, it can be used in the production of polyurethanes, fo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/18C12R1/22
Inventor 刘德华周胜罗吉安
Owner TSINGHUA UNIV
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