Method for preparing fatty acid by fermenting lignocellulose ionic liquid hydrolysate

An ionic liquid and fatty acid technology, applied in the field of microbial fermentation, can solve problems such as unreported fatty acids, and achieve the effects of alleviating the shortage of petrochemical resources, regulating sugar prices, and preventing waste.

Inactive Publication Date: 2015-08-12
CHONGQING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the production of fatty acids by fermentation of lignocellulose hydrolyzate, especially bamboo powder.

Method used

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  • Method for preparing fatty acid by fermenting lignocellulose ionic liquid hydrolysate
  • Method for preparing fatty acid by fermenting lignocellulose ionic liquid hydrolysate
  • Method for preparing fatty acid by fermenting lignocellulose ionic liquid hydrolysate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Effects of hydrolyzate obtained by different treatment methods on the production of fatty acids by fermentation of bamboo powder

[0040] The bamboo powder is hydrolyzed by ionic liquid through acid hydrolysis method and enzymatic hydrolysis method to obtain lignocellulose hydrolyzate.

[0041] (1) Acid hydrolysis method: After a sufficient amount of ionic liquid is melted, stir and react with bamboo powder, cupric chloride and 10% (v / v) hydrochloric acid at 100°C for 4-5 hours, add 50% (w / w) hydrogen after suction filtration Sodium oxide made it layered, mixed quickly for 30 minutes, and the supernatant obtained by rapid centrifugation with a centrifuge was the hydrolyzate used for fermentation.

[0042] (2) Enzyme hydrolysis method: After a sufficient amount of ionic liquid and bamboo powder are stirred and reacted at 120°C for 3 hours, add water to filter, and wash with water 4 to 6 times to obtain treated cellulose, dry at 80°C for 6 hours, and then add cellulase R...

Embodiment 2

[0048] Effects of different initial total sugar concentrations on fatty acid production from lignocellulose hydrolyzate

[0049] Use enzymatic hydrolysis method to obtain lignocellulose ionic liquid hydrolyzate: a sufficient amount of ionic liquid and bamboo powder are stirred and reacted at 120°C for 3 hours, then add water for suction filtration, and wash with water for 4 to 6 times to obtain treated cellulose, and dry at 80°C for 6 hours Afterwards, cellulase was added to react for 24 hours in an acetic acid-sodium acetate buffer solution with a pH of 4.8. The hydrolyzate obtained by suction filtration is used for fermentation.

[0050] Insert the E. coli strain into the seed culture medium, and culture it statically at 37°C for 18-20 hours in an ordinary incubator, and transfer it into a 250mL Erlenmeyer flask containing fermentation medium with different initial total sugar concentrations according to the inoculum size of 8%, and fill it with liquid The volume is 40mL, t...

Embodiment 3

[0055] Effects of Different Nitrogen Sources on Fatty Acid Production by Lignocellulose Hydrolyzate Fermentation

[0056] Use enzymatic hydrolysis method to obtain lignocellulose ionic liquid hydrolyzate: sufficient amount of ionic liquid and bamboo powder are stirred and reacted at 120°C for 3 hours, then add water for suction filtration, and wash with water for 4-6 times to obtain pretreated cellulose, and dry at 80°C After 6 hours, cellulase was added to react for 24 hours in an acetic acid-sodium acetate buffer solution with a pH of 4.8. The hydrolyzate obtained by suction filtration is used for fermentation.

[0057] According to the method for fatty acid production by Escherichia coli strain fermentation in Example 2, the DQ430 strain plate constructed in the laboratory was cultivated in a common box for 24 hours, and then connected to a 30mL seed medium with an inoculation loop, and cultivated at 37°C for 20h, according to 8% The inoculum amount was connected to a 250m...

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Abstract

The invention provides a method for producing fatty acid by fermenting lignocellulose ionic liquid hydrolysate. An escherichia coli DQ430 is utilized to regulate the total polysaccharide concentration of hydrolysate to be 10-50 g/L with water under an aerobic condition, fatty acid is produced by fermenting in batches or in fed-batch, and after fermenting for 24-72 hours, the concentration of fatty acid can be up to 3-15 g/L; the utilization rate of sugar is 60-90%, and the production intensity is up to 0.063-0.313g/L<-h>. The method for producing the fatty acid by fermenting the lignocellulose ionic liquid hydrolysate has the outstanding advantages that bamboo powder is used as a raw material to replace expansive glucose and is utilized as a carbon source to product fatty acid through fermentation; regenerated biomass resources are utilized, the environmental friendliness is achieved, and the shortage of petrifaction resource for chemic synthesis of fatty acid can be released.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, and in particular relates to a method for producing fatty acid by fermenting lignocellulose ionic liquid hydrolyzate, and at the same time relates to the application of Escherichia coli in the method for preparing fatty acid with bamboo powder as raw material. Background technique [0002] Fatty acid refers to a long aliphatic hydrocarbon chain containing a carboxyl group at one end. The general formula of straight-chain saturated fatty acid is C (n) h (2n+1) COOH, the lower fatty acid is a colorless liquid with a pungent odor, and the higher fatty acid is a waxy solid with no obvious smell. Fatty acids are the simplest type of lipids and are the building blocks of many more complex lipids. Fatty acids can be oxidized and decomposed into CO in the presence of sufficient oxygen supply 2 and H 2 O, release a lot of energy, so fatty acid is one of the main energy sources of the bo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/40C12R1/19
CPCC12P7/6409
Inventor 王丹秦丹丹邹环泽汪楠周小华
Owner CHONGQING UNIV
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