Fermentation production method of C19-C21 long-chain dibasic acids

A technology of long-chain dibasic acid and production method, applied in the fields of biotechnology and biological fermentation, can solve the problems of increased production cost, reduced utilization rate of fermentation substrate of fermentation strain, low yield of long-chain dibasic acid, etc.

Active Publication Date: 2019-05-03
CATHAY R&D CENT CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the industrial production of dibasic acids, in order to maintain the optimum fermentation temperature (29°C-32°C) of the commonly used fermentation strain Candida tropicalis, it is necessary to use a large amount of cooling water to cool down the fermentation system. When the ambient temperature is high, it is used for The cooling water also needs to be cooled in advance, which will greatly increase the power consumption, equipment investment, and personnel investment of the binary acid fermentation production, resulting in an increase in production costs, and is not conducive to energy saving and emission reduction
More importantly, the fermentation substrates (long-chain alkanes, fatty acids and their derivatives) of long-chain dibasic acids (such as C19, C20, and C21) whose carbon chain length exceeds C18 are solid or semi-solid at lower temperatures. Existence in solid form greatly reduces the utilization rate of the fermentation substrate for the fermentation strain, resulting in a lower conversion rate of the fermentation substrate and a lower yield of C19-C21 long-chain dibasic acids

Method used

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  • Fermentation production method of C19-C21 long-chain dibasic acids

Examples

Experimental program
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Effect test

Embodiment 1

[0055] Stability Verification of Example 1 Candida tropicalis 10468

[0056] The Candida tropicalis 10468 of the present invention has good stability in shape, growth and production performance as verified by continuous passage experiments. After five passages, there was no obvious change in the colony morphology: the surface of the colony was smooth and moist, milky white, shiny, round, and the edges were neat (such as figure 1 and figure 2 shown). The results showed that the passage stability of Candida tropicalis 10468 was good.

[0057] This embodiment also provides a method for fermenting Candida tropicalis 10468 to produce dodecanedioic acid (DC12), specifically as follows:

[0058] The first-generation and sixth-generation strains were subjected to shake-flask fermentation to verify the performance of producing DC12. A glycerol tube seed of Candida tropicalis 10468 was inserted into the YPD activation medium, cultured for 24 hours, and then inoculated into the seed...

Embodiment 2

[0061] This example provides a method for fermenting Candida tropicalis 10468 to produce DC19, specifically as follows:

[0062] Take a glycerol tube seed of Candida tropicalis 10468 and put it into the YPD activation medium, cultivate it for 24 hours, then put the culture solution into the seed medium. The formula of the seed medium is as follows: sucrose 20g / L, corn steep liquor 2g / L, yeast extract 6g / L, potassium dihydrogen phosphate 8g / L, urea 2g / L, n-alkane n-C1933mL / L; cultivated at 33°C 48h, measured the OD of the seed solution 620 When it reached 0.8 (measured after 30-fold dilution), the seed solution was inoculated into the shake flask containing the fermentation medium. The fermentation medium includes the formula as follows: sucrose 40g / L, corn steep liquor 5g / L, yeast extract 5g / L, potassium dihydrogen phosphate 8g / L, potassium nitrate 4g / L, sodium chloride 1.5g / L, urea 0.5g / L L. The substrate n-alkane n-C19 was added to the fermentation medium in an amount of ...

Embodiment 3

[0064] This example provides a method for fermenting Candida tropicalis 10468 to produce DC19, specifically as follows:

[0065] Take a glycerol tube seed of Candida tropicalis 10468 and put it into the YPD activation medium, cultivate it for 24 hours, then put the culture solution into the seed medium. The formulation of the seed medium is as follows: sucrose 20g / L, corn steep liquor 2g / L, yeast extract 6g / L, potassium dihydrogen phosphate 8g / L, urea 2g / L, n-alkane n-C1933mL / L; cultured at 35°C 48h, measured the OD of the seed solution 620 When it reached 0.8 (measured after 30-fold dilution), the seed solution was inoculated into the shake flask containing the fermentation medium. The formula of the fermentation medium is as follows: sucrose 40g / L, corn steep liquor 5g / L, yeast extract 5g / L, potassium dihydrogen phosphate 8g / L, potassium nitrate 4g / L, sodium chloride 1.5g / L, urea 0.5g / L . The substrate n-alkane n-C19 was added to the fermentation medium in an amount of 23...

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Abstract

The present invention relates to a fermentation production method of C19-C21 long-chain dibasic acids. The present invention discloses a method for producing the C19-C21 long-chain dibasic acids by fermentation of candida tropicalis 10468. The candida tropicalis 10468 can grow under high temperature conditions, can also efficiently convert C19-C21 normal alkanes with different carbon chain lengths, linear saturated fatty acids and linear saturated fatty acid derivatives into the C19-C21 long-chain dibasic acids, achieves high-temperature fermentation and efficient production of the C19-C21 long-chain dibasic acids, and can be used for the fermentation production of the C19-C21 long-chain dibasic acids in practice.

Description

technical field [0001] The invention relates to the fields of biotechnology and biological fermentation, in particular to a fermentation production method of C19-C21 long-chain dibasic acids. Background technique [0002] Long-chain dibasic acid is an important raw material for the synthesis of spices, nylon engineering plastics, hot-melt adhesives, resins, cold-resistant plasticizers, medicines and pesticides, etc. Its structural formula is HOOC(CH 2 )nCOOH, wherein n is an integer ≥9. Dodecanedibasic acid (DC12) and tetradecanedibasic acid (DC14) in the long-chain dibasic acid are important for the synthesis of advanced nylon engineering plastics with special properties and wide applications - nylon 1212 and nylon 1414. raw material. Long-chain dibasic acids with more than twelve carbons do not exist in nature and need to be artificially designed and synthesized. The chemical synthesis of long-chain dibasic acids with more than twelve carbons has problems such as long r...

Claims

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Application Information

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IPC IPC(8): C12P7/44C12R1/74
Inventor 汪江林李葳李秦佩刘修才
Owner CATHAY R&D CENT CO LTD
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