Microbial compound bacterial agent, microencapsulated compound microecological preparation, meat rabbit feed, preparation method and application

A technology of compound microecology and compound bacterial agent, applied in the field of microorganisms, can solve problems such as difficult to open, insufficient number and vitality of bacteria

Active Publication Date: 2021-07-27
天津开发区坤禾生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the probiotics themselves are relatively fragile and are greatly affected by the surrounding environmental factors, the number and vitality of the bacteria colonized in the intestinal tract of animals are not enough, and it is difficult to achieve the desired effect

Method used

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  • Microbial compound bacterial agent, microencapsulated compound microecological preparation, meat rabbit feed, preparation method and application
  • Microbial compound bacterial agent, microencapsulated compound microecological preparation, meat rabbit feed, preparation method and application
  • Microbial compound bacterial agent, microencapsulated compound microecological preparation, meat rabbit feed, preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] (1) High-density liquid fermentation of Bacillus lentus:

[0044] (1) Strain preparation: Take a glycerol tube of Bacillus lentus MES857 stored at -80°C. After thawing, perform three-section lines on the nutrient agar medium, and purify 3-5 times to obtain a single colony.

[0045] (2) Strain activation: Use a sterile inoculation loop to pick up the bacterial lawn on the slant of the test tube, streak evenly on the 250 mL eggplant bottle slant medium, and cultivate at 28°C until complete sporulation.

[0046] Bacillus lentus MES857 strain preparation and activation medium, specifically, by mass fraction: tryptone 0.8%, beef extract 1.5%, NaCl 0.3%, MnSO 4 ·H 2 O 0.05%, agar 1.5%, the rest is deionized water, pH 7.0, sterilized at 121°C for 20min.

[0047] (3) Preparation of seed solution: Wash the bacterial lawn on the slope of the eggplant bottle with 100 mL of sterile water to prepare a uniform bacterial suspension.

[0048] (4) Primary seed tank fermentation: Tran...

Embodiment 2

[0070] (1) High-density liquid fermentation of Bacillus lentus:

[0071] (1) Strain preparation: Take a glycerol tube of Bacillus lentus MES857 stored at -80°C. After thawing, perform three-section lines on the nutrient agar medium, and after purification for 3 to 5 times, a single colony is obtained.

[0072] (2) Activation of strains: Use a sterile inoculation loop to pick up the bacterial lawn on the slant of the test tube, evenly streak on the 250mL eggplant bottle slant medium, and culture at 33°C until complete sporulation.

[0073]Bacillus lentus MES857 strain preparation and activation medium, specifically, by mass fraction: tryptone 1%, beef extract 0.8%, NaCl 0.3%, MnSO 4 ·H 2 O 0.01%, agar 1.5%, the rest is deionized water, pH 6.5, sterilized at 121°C for 30min.

[0074] (3) Preparation of seed liquid: Wash the bacterial lawn of the eggplant bottle with 100 mL of sterile water to prepare a uniform bacterial suspension.

[0075] (4) Primary seed tank fermentation:...

Embodiment 3

[0098] (1) High-density liquid fermentation of Bacillus lentus:

[0099] (1) Strain preparation: Take a glycerol tube of Bacillus lentus MES857 stored at -80°C. After thawing, perform three-section lines on the nutrient agar medium, and after purification for 3 to 5 times, a single colony is obtained.

[0100] (2) Strain activation: Use a sterile inoculation loop to pick up the bacterial lawn on the slant of the test tube, streak evenly on the 250 mL eggplant bottle slant medium, and cultivate at 28°C until complete sporulation.

[0101] Bacillus lentus MES857 strain preparation and activation medium, specifically, by mass fraction: tryptone 0.8%, beef extract 0.8%, NaCl 0.5%, MnSO 4 ·H 2 O 0.01%, agar 2%, the rest is deionized water, pH 7.0, sterilized at 121°C for 20min.

[0102] (3) Preparation of seed liquid: Wash the bacterial lawn of the eggplant bottle with 50 mL of sterile water to prepare a uniform bacterial suspension.

[0103] (4) Primary seed tank fermentation: ...

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Abstract

The invention provides a microbial compound bacterial agent, a microencapsulated compound microecological agent, meat rabbit feed, a preparation method and an application thereof, and relates to the technical field of microorganisms. The microbial compound agent includes Bacillus lentus and Bacillus Veles. It can significantly improve the intestinal micro-ecological environment of animals, increase the conversion rate of meat rabbit feed, and achieve the purpose of rapid weight gain. The microencapsulated composite micro-ecological preparation provided by the present invention selects the microbial composite bacterial agent as the core material, and the wrapped probiotics can be released in the intestinal tract of animals, which can resist adverse environments such as high gastric acid and bile salts in the host body, and are more convenient for transportation and long-term storage. The meat rabbit feed provided by the invention can obviously improve the utilization rate of the meat rabbit feed and shorten the growth period of the meat rabbit.

Description

technical field [0001] The invention relates to the technical field of microbes, and specifically provides a microbial compound bacterial agent, a microencapsulated compound microecological agent, meat rabbit feed, a preparation method and an application thereof. Background technique [0002] With the rapid development of the aquaculture industry, probiotic products have attracted more and more attention. Probiotics are also known as prebiotics and probiotics. As a safe and non-toxic substitute for antibiotics, probiotics can not only improve the production of livestock and poultry farming and the quality of animal products, but also can replace antibiotics. A large number of studies have confirmed that probiotics have a variety of physiological functions, such as: enhancing the immune function of the body, regulating the micro-ecological environment of the intestinal tract of animals, inhibiting the reproduction of harmful bacteria, improving the conversion rate of feed, ma...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23K10/18A23K40/30A23K50/50A23K40/10A23K10/30A23K10/37A23K20/142A23K20/20C12R1/07
CPCA23K10/18A23K10/30A23K10/37A23K20/142A23K20/20A23K40/10A23K40/30A23K50/50C12N1/20Y02P60/87
Inventor 刘金龙孙海远王淑会刘刚
Owner 天津开发区坤禾生物技术有限公司
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