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Pretreatment method for producing butanol by fermentation of bagasse

A technology for pretreatment and bagasse is applied in the field of pretreatment using bagasse to ferment butanol, and achieves the effects of low energy consumption, low content and mild pretreatment conditions

Active Publication Date: 2015-02-18
BIOGAS SCI RES INST MIN OF AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In the prior art, there is no pretreatment method that can avoid or reduce the production of all major inhibitors

Method used

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  • Pretreatment method for producing butanol by fermentation of bagasse
  • Pretreatment method for producing butanol by fermentation of bagasse
  • Pretreatment method for producing butanol by fermentation of bagasse

Examples

Experimental program
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Effect test

Embodiment 1

[0048] 1) Cut 500g of sugarcane to a length of 5-10cm, dry at 65±2°C for 2 days, then pulverize and pass through a 20-mesh sieve to obtain bagasse powder with a particle size of less than 830μm;

[0049] 2) Soak the product obtained in step 1) in 2L of water at room temperature for 12 hours, then preheat with steam for 45 seconds, then add liquid hot water, react at 200±3°C for 1 hour, filter after reaction, collect filtrate I and solid residue I;

[0050] 3) Adjust the humidity of the solid residue obtained in step 2) to 40%, and place it in a three-necked round-bottomed flask, then pass through nitrogen to exclude oxygen, and place it at 4 °C for 12 hours, and then place the reactor in a microwave pyrolysis furnace React at 120 °C for 7 min, discharge the hot gas, soak the reaction product with 90% ethanol after the temperature drops to room temperature, and then filter to collect the filtrate II and solid residue II;

[0051] 4) Dry the solid residue obtained in ste...

Embodiment 2

[0058] 1) Cut 500g of sugarcane to a length of 5-10cm, dry at 65±2°C for 2 days, then pulverize and pass through a 20-mesh sieve to obtain bagasse powder with a particle size of less than 830μm;

[0059] 2) Soak the product obtained in step 1) in 2L of water at room temperature for 12 hours, then preheat with steam for 45 seconds, then add liquid hot water, react at 200±3°C for 1 hour, filter after reaction, collect filtrate I and solid residue I;

[0060] 3) Adjust the humidity of the solid residue obtained in step 2) to 40%, and place it in a three-necked round-bottomed flask, then pass through nitrogen to exclude oxygen, and place it at 4 °C for 8 hours, and then place the reactor in a microwave pyrolysis furnace React at 120 °C for 7 min, discharge the hot gas, soak the reaction product with 90% ethanol after the temperature drops to room temperature, and then filter to collect the filtrate II and solid residue II;

[0061] 4) Dry the solid residue obtained in step...

Embodiment 3

[0068] 1) Cut 500g of sugarcane to a length of 5-10cm, dry at 65±2°C for 2 days, then pulverize and pass through a 20-mesh sieve to obtain bagasse powder with a particle size of less than 830μm;

[0069] 2) Soak the product obtained in step 1) in 2L of water at room temperature for 12 hours, then preheat with steam for 45 seconds, then add liquid hot water, react at 200±3°C for 1 hour, filter after reaction, collect filtrate I and solid residue I;

[0070] 3) Adjust the humidity of the solid residue obtained in step 2) to 40%, and place it in a three-necked round-bottomed flask, then pass through nitrogen to exclude oxygen, and place it at 4 °C for 10 hours, and then place the reactor in a microwave pyrolysis furnace React at 120 °C for 7 min, discharge the hot gas, soak the reaction product with 90% ethanol after the temperature drops to room temperature, and then filter to collect the filtrate II and solid residue II;

[0071] 4) Dry the solid residue obtained in ste...

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Abstract

The invention discloses a pretreatment method for producing butanol by fermentation of bagasse. The pretreatment method comprises the following steps of mechanical grinding, liquid-state hot-water treatment, microwave treatment, microbial decomposition, ammonia treatment and enzymolysis treatment, after the steps are performed, the decomposition products in each step can be mixed and subjected to step-by-step saccharification and fermentation and simultaneous saccharification and fermentation. By the pretreatment method, the contents of major inhibitors furancarboxaldehyde and hydroxymethyl furfural can be controlled below the concentration without affecting the growth of fermentative bacteria and other inhibitors such as glucuronic acid, p-coumaric acid, syringic acid and ferulic acid are not basically produced. Since the detoxification purification treatment of the pretreated decomposition products is not needed and the pretreated decomposition products are directly subjected to biological fermentation to produce butanol, the pretreatment method has the advantages that the production of inhibitors can be controlled, the cost is low, the yield is high and the environment friendliness is achieved.

Description

technical field [0001] The invention relates to the field of biomass pretreatment, in particular to a pretreatment method for producing butanol by fermenting bagasse. Background technique [0002] At present, there are a variety of mature pretreatment methods for fibrous and semi-fibrous materials to hydrolyze sugars, which can be used for fermentation to produce biofuels. [0003] Recently, some advances in these methods have been made in the field of industrial biotechnology, providing potential opportunities for cost-effective utilization of agricultural industry wastes. For example, bagasse is a comprehensive material, a major by-product of the sugarcane industry and one of the major agricultural wastes, which is abundantly produced in southern China every year. Bagasse contains approximately 50% cellulose, 25% hemicellulose and 25% lignin, and its use to produce biobutanol is an ideal method for biofuel production because butanol has Higher calorific value and...

Claims

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Application Information

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IPC IPC(8): C12P7/16C12R1/685C12R1/885C12R1/80
CPCC12P7/16Y02E50/10
Inventor 谭芙蓉苏海锋何明雄胡启春
Owner BIOGAS SCI RES INST MIN OF AGRI
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