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Method for pretreating biomass by using dual-functionalized ionic liquid to improve enzymolysis efficiency

A technology for pretreatment of biomass and ionic liquid, which is applied in the field of utilization and treatment of agricultural and forestry biomass resources to achieve the effects of improving the yield of reducing sugar and enhancing the efficiency of enzymatic hydrolysis

Inactive Publication Date: 2017-01-04
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Ionic liquids containing sulfonic acid anion groups show better dissolution and extraction effects on lignin, but because ionic liquids are easy to absorb water, and sulfonic acid anion groups are easily hydrolyzed into acidic groups in the presence of water and heating. , such that cellulose and hemicellulose are prone to degradation under acidic conditions

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  • Method for pretreating biomass by using dual-functionalized ionic liquid to improve enzymolysis efficiency

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] [Et 4 N][Me 2 NC 4 SO 3 ] Pretreatment at 120℃ improves the efficiency of enzymatic hydrolysis of eucalyptus bark

[0039] (1) Pretreatment: Accurately weigh 500 mg of bark powder (40-80 mesh in particle size) and 10 g [Et 4 N][Me 2 NC 4 SO 3 ] ionic liquid, placed in a 100ml round bottom flask, stirred at 120°C for 10 hours; after cooling to room temperature, add 40ml of deionized water to wash, filter, and then wash the filter residue with 40ml of deionized water for 5 times, and remember to Pre-treated eucalyptus bark.

[0040] (2) Enzyme hydrolysis experiment: accurately weigh 20 mg of eucalyptus bark after pretreatment, place in a 50 ml Erlenmeyer flask with stopper, add 7 ml of citrate buffer (50 mmol / L, pH 4.8) and cellulase, seal and place React in a constant temperature oscillator at 160r / min at 50°C. 200ul samples were taken regularly, and then taken out and placed in boiling water to quench the enzymolysis reaction. After centrifugation, the concentr...

Embodiment 2

[0044] [Et 4 N][i-PrNHC 3 SO 3 ] Pretreatment at 120℃ improves the efficiency of enzymatic hydrolysis of eucalyptus bark

[0045] (1) Pretreatment: Accurately weigh 500 mg of bark powder (40-80 mesh in particle size) and 10 g [Et 4 N][i-PrNHC 3 SO 3 ] ionic liquid, placed in a 100ml round bottom flask, stirred at 120°C for 10 hours; after cooling to room temperature, add 40ml of deionized water to wash, filter, and then wash the filter residue with 40ml of deionized water for 5 times, and remember to Pre-treated eucalyptus bark.

[0046] (2) Enzyme hydrolysis experiment: accurately weigh 20 mg of eucalyptus bark after pretreatment, place in a 50 ml Erlenmeyer flask with stopper, add 7 ml of citrate buffer (50 mmol / L, pH 4.8) and cellulase, seal and place React in a constant temperature oscillator at 160r / min at 50°C. 200ul samples were taken regularly, and then taken out and placed in boiling water to quench the enzymolysis reaction. After centrifugation, the concentrat...

Embodiment 3

[0050] [Ch][Et 2 NC 4 SO 3 ] Pretreatment at 120℃ improves the efficiency of enzymatic hydrolysis of eucalyptus bark

[0051] (1) Pretreatment: Accurately weigh 500mg of eucalyptus bark powder (40-80 mesh in particle size) and 10g of [Ch][Et 2 NC 4 SO 3 ] ionic liquid, placed in a 100ml round bottom flask, stirred at 120°C for 10 hours; after cooling to room temperature, add 40ml of deionized water to wash, filter, and then wash the filter residue with 40ml of deionized water for 5 times, and remember to Pre-treated eucalyptus bark.

[0052] (2) Enzyme hydrolysis experiment: accurately weigh 20 mg of eucalyptus bark after pretreatment, place in a 50 ml Erlenmeyer flask with stopper, add 7 ml of citrate buffer (50 mmol / L, pH 4.8) and cellulase, seal and place React in a constant temperature oscillator at 160r / min at 50°C. 200ul samples were taken regularly, and then taken out and placed in boiling water to quench the enzymolysis reaction. After centrifugation, the concen...

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Abstract

The invention provides a method for pretreating biomass by using a dual-functionalized ionic liquid to improve enzymolysis efficiency and relates to the technical field of utilization and treatment of agricultural and forestry biomass resources. According to the method, firstly, the dual-functionalized ionic liquid containing a basic group and a sulfonic acid anion base is taken as a solvent, after native biomass is pretreated at a certain temperature for a certain period, residues are obtained through filtering separation and cleaning, and pretreated native biomass is obtained after drying. The treated native biomass is taken as a substrate and subjected to enzymolysis with cellulose, and a sugar liquid mainly containing glucose and xylose is obtained finally. The enzymolysis efficiency of the raw biomass can be effectively enhanced, the yield of fermentable reducing sugar is increased, compared with traditional organic solvents and other systems, the method is safe and non-toxic and has low requirement for experimental equipment, and the ionic liquid can be recycled.

Description

technical field [0001] The invention relates to the technical field of utilization and treatment of agricultural and forestry biomass resources, and invents a new method for improving enzymolysis efficiency by pretreating raw biomass with a dual-functional ionic liquid. Background technique [0002] With the shortage and price rise of non-renewable resources such as oil and coal, and people's concern about environmental pollution, biomass energy, which ranks fourth in the world's total energy consumption after coal, oil and natural gas, has attracted much attention. focus on. Biomass energy, as a clean and renewable energy, is the only carbon resource that can replace fossil energy into gaseous, liquid and solid fuels and other chemical raw materials or products. As a low-grade energy form, biomass is converted into high-grade liquid fuels, which is the most important research direction in the field of bioenergy. In the conversion process of lignocellulose, the bioconversi...

Claims

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Application Information

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IPC IPC(8): C12P19/14C12P19/02
CPCY02P20/54
Inventor 张宗超颜佩芳
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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