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Pre-processing method for improving enzymolysis saccharifying effect of biomass

A biomass and pretreatment technology, applied in fermentation and other directions, can solve the problem of inability to achieve effective separation of cellulose, hemicellulose and lignin at the same time, inability to fully and effectively utilize lignocellulose resources, and the high cost of producing lignocellulosic ethanol. It can improve the conversion and comprehensive utilization rate, improve the enzymatic hydrolysis performance, and shorten the enzymatic hydrolysis time.

Active Publication Date: 2015-06-24
HEBEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The current pretreatment process mostly uses a single technology, such as acid pretreatment or steam explosion to remove only hemicellulose, and alkali pretreatment to remove lignin and part of hemicellulose, which cannot achieve the effective removal of cellulose, hemicellulose and lignin at the same time. Separation, the use of cellulase in the subsequent enzymatic hydrolysis and saccharification of the pretreated cellulose solid part is large, and the saccharification efficiency is low, resulting in high cost of producing lignocellulosic ethanol, and it is impossible to realize the full and effective utilization of lignocellulosic resources. Exploitation and other defects

Method used

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Examples

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Effect test

Embodiment

[0027] The present invention proposes a pretreatment method for providing biomass enzymatic hydrolysis performance - a two-step variable temperature treatment method. The present invention can be implemented by the following methods:

[0028] Corn stalk powder crushed to 40 mesh (moisture content 3.2%) was used as raw material, and 200.00g H 2 o 2 The solution (mass percentage concentration: 3%) was added into a screw bottle with a stopper containing 20.00% corn stalk powder. The reacted mixture was subjected to solid-liquid separation to obtain hydrolyzate A and wet slag A, and the first step of pretreatment was completed; 90.00 g of hydrochloric acid pickling waste liquid diluted 6 times was weighed and added to a container containing 10.00 g of wet slag A. In a screw-top bottle, after airtight, cook in a high-pressure steam sterilizer at 130°C for 60 minutes, and then separate the mixture from solid to liquid after cooling to obtain hydrolyzate B and wet slag B, and comple...

Embodiment 2

[0031] Take bagasse powder crushed to 40 meshes (moisture content 3.1%) as raw material, weigh 180.00g N a2 o 2 The solution (mass percentage concentration is 10%) was added into a screw-top bottle with 20.00% cassava slag powder. The reacted mixture was subjected to solid-liquid separation to obtain hydrolyzate A and wet slag A, and the first step of pretreatment was completed; 90.00 g of sulfuric acid pickling waste liquid diluted 8 times was weighed and added to the reaction solution containing 10.00 g of wet slag A. Reactor (PCF02-10 / TA2 magnetic stirring reactor, stirring speed 300r / min), after airtight, react at 150°C for 10min, after the reaction is completed, take out the reactor and cool it in an ice-water bath. The mixture was subjected to solid-liquid separation to obtain hydrolyzate B and wet slag B, and the second step of pretreatment was completed; 8.86 g of wet slag obtained after the second step was washed with 300.00 g of normal temperature deionized water fo...

Embodiment 3

[0033] Rapeseed stalk powder crushed to 40 mesh (moisture content 3.5%) was used as raw material, and 200.00g H 2 o 2 The solution (mass percentage concentration: 2%) was added to a screw-top bottle with 20.00% rape stalk powder, and after sealing, it was reacted in a constant temperature oscillator (rotation speed: 100r / min, temperature: 80°C) for 4 hours. The reacted mixture is subjected to solid-liquid separation to obtain hydrolyzate A and wet slag A, and the first step of pretreatment is completed; 90.00 g of nitric acid pickling waste liquid diluted 10 times is weighed and added to a container containing 10.00 g of wet slag A. In a screw-top bottle, after airtight, cook in a high-pressure steam sterilizer at 110°C for 60 minutes, and then separate the mixture from solid to liquid after cooling to obtain hydrolyzate B and wet slag B, and complete the second step of pretreatment; The 8.26g of wet residue B obtained after the two-step treatment was washed with 300.00g of n...

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Abstract

The invention relates to a pre-processing method for improving the enzymolysis saccharifying effect of biomass. According to the method, peroxidate pre-processing is used for enabling lignose to be degraded or peeled off from biomass raw material, so as to keep most of cellulose and hemicellulose, then steel pickling waste liquor pre-processing is used for enabling hemicellulose in solid raw material to be hydrolyzed sufficiently, only residue with high cellulose content is left, the cellulose of the residue is excellent in enzymolysis performance, the usage amount of cellulase can be effectively reduced, and the enzymolysis time is shortened greatly, therefore, the enzymolysis saccharification cost is reduced; meanwhile, soluble sugar converted from high yield hemicellulose can be obtained during the pre-processing process, the soluble sugar can be used for production of energy or other chemical products, and the conversion rate and the rate of multipurpose utilization of the biomass can be further improved.

Description

technical field [0001] The invention relates to biomass pretreatment, in particular to a biomass enzymatic saccharification or other biorefining pretreatment method. Background technique [0002] As the international crude oil price keeps rising and the domestic demand for oil is increasing day by day, bioethanol, as a renewable energy, has become a new type of energy promoted by the government. Since lignocellulosic biomass is rich in cellulose and hemicellulose, and can be degraded into fermentable glucose and xylose, etc., if biomass with abundant sources, low price and strong regeneration is used as raw material for bioethanol production Production not only avoids the problem of "competing with others for food" caused by grain ethanol, but also provides a good way to utilize wood cellulosic waste. Therefore, lignocellulosic wastes such as crop straws are considered as the most potential raw materials for bioethanol production, and have received extensive attention from ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/14C12P19/02
Inventor 孙优善黄超马秀琴张长平王美艳
Owner HEBEI UNIV OF TECH
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