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Method for preparing guar gum oligogalactomannan enzymolysis solution by composite enzyme process

A technology of mannose enzymatic hydrolysis solution and compound enzymatic method, which is applied in the field of preparing guar gum oligogalactomannose enzymatic hydrolysis solution, can solve the problems of long time, low enzymatic hydrolysis efficiency, low oligosaccharide and the like, and saves energy Production time and cost, no toxic side effects, good enzymatic hydrolysis effect

Inactive Publication Date: 2015-06-17
QINGZHOU RONMER BIOLOGY TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

One is that the existing method mainly adopts β-mannanase to the enzymatic hydrolysis of guar gum, but the efficiency of adopting a single enzyme to hydrolyze guar gum is not high
Although some existing technologies use the method of degrading plant polysaccharides with compound enzymes, since each enzyme degrades separately, it takes a long time and the enzymatic hydrolysis efficiency is low
The 2nd, in the guar gum enzymatic hydrolysis process, because the guar gum solution viscosity is too high, the concentration during the enzymolysis guar gum that generally adopts at present is 0.3-2%, the concentration of the oligosaccharide that obtains is lower, in further When preparing galactomannose powder, dehydration and drying treatment is required, which not only increases the cost of subsequent dehydration and drying of the product, galactomannose, but also seriously affects the efficiency of industrial production

Method used

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  • Method for preparing guar gum oligogalactomannan enzymolysis solution by composite enzyme process
  • Method for preparing guar gum oligogalactomannan enzymolysis solution by composite enzyme process
  • Method for preparing guar gum oligogalactomannan enzymolysis solution by composite enzyme process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Mix 8 g of guar gum powder with 400 U of β-mannanase, 8000 U of endoglucanase, and 8000 U of cellobiase; The pH value is 6.0; the solution is heated to 22°C and swells for 30 minutes. Then the temperature of the solution was raised to 50° C., and the enzymatic hydrolysis was carried out for 6 hours; after the end of the enzymatic hydrolysis, an enzymatic hydrolysis solution of oligogalactomannose was obtained. The mass percentages of galactomannose oligosaccharides with different degrees of polymerization in the enzymatic hydrolysis solution of this example measured by MALDI-TOF and HPLC methods are shown in Table 1. 95.7% of the composition.

[0028] Table 1 The mass percent of galactomannose with different degrees of polymerization in Example 1

[0029]

Embodiment 2

[0031] Mix 10 g of guar gum powder with 600 U of β-mannanase, 20,000 U of endoglucanase, and 15,000 U of cellobiase; The pH value is 6.3; the temperature of the solution is raised to 26°C, and it is swelled for 60 minutes; then the temperature of the solution is raised to 52.5°C, and the enzymolysis is performed for 8 hours; The mass percentages of galactomannose oligosaccharides with different degrees of polymerization in the enzymatic hydrolyzate of this example measured by MALDI-TOF and HPLC methods are shown in Table 2, and galactomannose oligosaccharides with a degree of polymerization of 2-10 account for the sugars in the enzymatic hydrolyzate 96.4% of the composition.

[0032] Table 2 The mass percent of galactomannose with different degrees of polymerization in Example 2

[0033]

Embodiment 3

[0035] 12g of guar gum powder was fully mixed with 840U of β-mannanase, 36000U of endoglucanase and 24000U of cellobiase; The pH value is 6.6; the solution is warmed up to 30°C and swells for 90min. Then the temperature of the solution was raised to 55° C., and the enzymatic hydrolysis was carried out for 10 hours; after the end of the enzymatic hydrolysis, an enzymatic hydrolysis solution of oligogalactomannose was obtained. The mass percentages of galactomannose oligosaccharides with different degrees of polymerization in the enzymatic hydrolysis solution of this example measured by MALDI-TOF and HPLC methods are shown in Table 3, and galactomannose oligosaccharides with a degree of polymerization of 2-10 account for the sugars in the enzymatic hydrolysis solution 98.4% of ingredients.

[0036] Table 3 The mass percent of galactomannose with different degrees of polymerization in Example 3

[0037]

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Abstract

The invention discloses a method for preparing a guar gum oligogalactomannan enzymolysis solution by a composite enzyme process, which comprises the following steps: thoroughly mixing guar gum powder with beta-mannase, endoglucanase and cellobiase; adding a citric acid-sodium citrate buffer solution to prepare a mixed solution of the guar gum and three enzymes; and after the guar gum swells, controlling the enzymolysis temperature and time to perform enzymolysis. The method implements simultaneous enzymolysis of the three composite enzymes, omits the purification step after sequential degradation of single enzymes, saves the production time and cost and enhances the efficiency. The method implements enzymolysis of the guar gum under high-concentration conditions, obtains the oligogalactomannan enzymolysis solution, and saves the dehydration and drying cost of the subsequent oligogalactomannan production process.

Description

technical field [0001] The invention relates to a method for preparing guar gum oligogalactomannose enzymatic hydrolysis solution by using a compound enzyme method, and belongs to the field of biotechnology. Background technique [0002] Guar gum is a macromolecular natural hydrophilic colloid, which is processed from the endosperm of guar beans. The main ingredient is galactomannan, which is a natural thickener and quality improver. The appearance is white or yellowish free-flowing powder, which can be dissolved in cold water or hot water, and forms a gel-like substance when it meets water, achieving the effect of rapid thickening. The linear backbone of galactomannans consists of mannose sugars linked together by β-(1-4) glycosidic bonds, with α-(1-6) glycosidic bonds forming branch points linking galactose units. Galactomannose is an incomplete degradation product of galactomannan. It is a general term for oligosaccharides polymerized by 2-10 galactose and mannose molec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14
Inventor 刘云国张亮荣宝刚
Owner QINGZHOU RONMER BIOLOGY TECH CO LTD
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