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Feed compound enzyme containing neutral protease and preparation method thereof

A technology of neutral protease and feed compound enzyme, which is applied in the field of feed compound enzyme containing neutral protease and its preparation, and can solve problems such as unmeasured and complex structure

Active Publication Date: 2014-06-18
HUNAN HONGYING BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main reason is that the structure of the market is relatively complex, the market is diversified, and it is even impossible to measure

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] A feed compound enzyme containing neutral protease, consisting of the following enzyme preparations in parts by weight:

[0072] Concentrated maltase 35 parts, acid protease 25 parts, neutral protease 25 parts, acid xylanase 25 parts, cellulase 25 parts, β-glucanase 17 parts, pectinase 17 parts, amylase 17 parts , 12 parts of mannosidase, 13 parts of phytase, 12 parts of Chinese herbal medicine extract, 13 parts of protective agent, and 12 parts of activator.

[0073] Described acid protease, neutral protease, acid xylanase, cellulase, β-glucanase, pectinase, amylase, mannase, phytase are all food-grade enzyme preparations;

[0074] The preparation method of described neutral protease comprises the steps:

[0075] (1) Inoculate the well-preserved slant strain of Bacillus subtilis 1398-2-12 on the slant medium, culture at 30°C for 24 hours to activate the strain, and activate it twice;

[0076] The slant medium consists of: beef extract 3g, sodium chloride 5g, peptone ...

Embodiment 2

[0126] A feed compound enzyme containing neutral protease, consisting of the following enzyme preparations in parts by weight:

[0127] Concentrated maltase 30 parts, acid protease 20 parts, neutral protease 20 parts, acid xylanase 20 parts, cellulase 20 parts, β-glucanase 15 parts, pectinase 15 parts, amylase 15 parts , 10 parts of mannosidase, 10 parts of phytase, 10 parts of Chinese herbal medicine extract, 10 parts of protective agent, and 10 parts of activator.

[0128] Described acid protease, neutral protease, acid xylanase, cellulase, β-glucanase, pectinase, amylase, mannase, phytase are all food-grade enzyme preparations;

[0129] The preparation method of described neutral protease comprises the steps:

[0130] (1) Activation of strains

[0131] The well-preserved slant strain of Bacillus subtilis 1398-2-12 was inoculated on the slant medium, cultured at 33°C for 30 hours to activate the strain, and activated twice in this way;

[0132] The slant medium consists o...

Embodiment 3

[0181] A feed compound enzyme containing neutral protease, consisting of the following enzyme preparations in parts by weight:

[0182] Concentrated maltase 40 parts, acid protease 30 parts, neutral protease 30 parts, acid xylanase 30 parts, cellulase 30 parts, β-glucanase 20 parts, pectinase 20 parts, amylase 20 parts , 15 parts of mannosidase, 15 parts of phytase, 15 parts of Chinese herbal medicine extract, 15 parts of protective agent, and 15 parts of activator.

[0183] Described acid protease, neutral protease, acid xylanase, cellulase, β-glucanase, pectinase, amylase, mannase, phytase are all food-grade enzyme preparations;

[0184] The preparation method of described neutral protease comprises the steps:

[0185] (1) Activation of strains

[0186] The well-preserved slant strain of Bacillus subtilis 1398-2-12 was inoculated on the slant medium, cultured at 36°C for 36 hours to activate the strain, and activated 3 times in this way;

[0187] The slant medium consists...

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Abstract

The invention discloses a feed compound enzyme containing neutral protease and a preparation method thereof, and belongs to the technical field of preparation of an enzyme preparation. The feed compound enzyme containing neutral protease is prepared from concentrated malt enzyme, acid proteinase, neutral protease, acidic xylanase, cellulose, beta-glucanase, pectinase, amylase, seminase, phytase, Chinese herb extracts, a protective agent and an activating agent in a scientific distribution manner. The feed compound enzyme is high in enzyme activity, wide in action condition, strong in stability, and more applicable to feed production. A safe digestive enzyme can be provided for beasts and birds, the digestion burden is relieved, the utilization rate and the growth rate of the raw materials are improved, the environment is protected, joyful natural malt fragrance is brought for the feed, the livestock and poultry appetite is enhanced, meanwhile, the efficacy of the enzyme preparation can be fully played by proper quantity of activating agent under the same condition, the adding amount of the enzyme preparation is saved, the expiration date of the compound enzyme preparation is prolonged by scientific distribution of the Chinese herb extracts, and the immunity of the fed beasts and birds can be improved.

Description

technical field [0001] The invention belongs to the technical field of preparation of enzyme preparations, in particular to a feed compound enzyme containing neutral protease and a preparation method thereof. Background technique [0002] There are four main reasons for adding enzyme preparations to feed: 1. Degradation of anti-nutritional factors present in animal feed. These substances cannot be degraded by animal endogenous enzymes, thereby interfering with the normal metabolism of animals, resulting in indigestion of animals and decreased production performance. 2. Improve the utilization rate of starch, protein and minerals. These substances are either surrounded by fiber-rich cell walls, or are present in certain structures that cannot be digested by animals (for example, in plant feed materials, a large amount of phosphorus is present in the form of phytate phosphorus.). 3. Degrade some specific chemical bonds in raw materials. These chemical bonds cannot be degrad...

Claims

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Application Information

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IPC IPC(8): A23K1/165
Inventor 李洪兵李贵骏杨中秋孙霞王章林王永兰
Owner HUNAN HONGYING BIOTECHNOLOGY CO LTD
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