A kind of wheat dietary enzyme containing neutral protease and preparation method thereof
A neutral protease and acid protease technology, applied in the field of wheat dietary enzymes and their preparation, can solve the problems of incalculability and complex structure, and achieve the effect of wide applicable pH value range and high temperature tolerance
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0057] A kind of wheat dietary enzyme containing neutral protease, is made up of the enzyme preparation of following parts by weight:
[0058] Bacillus subtilis culture 25 parts, acid protease 25 parts, acid xylanase 25 parts, cellulase 25 parts, β-glucanase 17 parts, amylase 17 parts, β-mannanase 12 parts, 12 parts of Chinese herbal medicine extract, 13 parts of protective agent, 12 parts of activator.
[0059] The acid protease, acid xylanase, cellulase, β-glucanase, amylase, and β-mannanase are all food-grade enzyme preparations;
[0060] The preparation method of described bacillus subtilis culture comprises the steps:
[0061] (1) Inoculate the well-preserved slant strain of Bacillus subtilis 1398-2-12 on the slant medium, culture at 30°C for 24 hours to activate the strain, and activate it twice;
[0062] The slant medium consists of: beef extract 3g, sodium chloride 5g, peptone 10g, glucose 2g, (NH) 2 SO 4 3g,K 2 HPO 4 6g, CaCl 2 1g, 15g agar, 5g Chinese herbal m...
Embodiment 2
[0096] A kind of wheat dietary enzyme containing neutral protease, is made up of the enzyme preparation of following parts by weight:
[0097] 20 parts of Bacillus subtilis culture, 20 parts of acid protease, 20 parts of acid xylanase, 20 parts of cellulase, 15 parts of β-glucanase, 15 parts of amylase, 10 parts of β-mannanase, 10 parts of Chinese herbal medicine extract, 10 parts of protective agent, 10 parts of activator.
[0098]The acid protease, acid xylanase, cellulase, β-glucanase, amylase, and β-mannanase are all food-grade enzyme preparations;
[0099] The preparation method of described bacillus subtilis culture comprises the steps:
[0100] (1) Activation of strains
[0101] The well-preserved slant strain of Bacillus subtilis 1398-2-12 was inoculated on the slant medium, cultured at 33°C for 30 hours to activate the strain, and activated twice in this way;
[0102] The slant medium consists of: beef extract 6g, sodium chloride 8g, peptone 15g, glucose 4g, (NH) ...
Embodiment 3
[0135] A kind of wheat dietary enzyme containing neutral protease, is made up of the enzyme preparation of following parts by weight:
[0136] 30 parts of Bacillus subtilis culture, 30 parts of acid protease, 30 parts of acid xylanase, 30 parts of cellulase, 20 parts of β-glucanase, 20 parts of amylase, 15 parts of β-mannanase, 15 parts of Chinese herbal medicine extract, 15 parts of protective agent, 15 parts of activator.
[0137] The acid protease, acid xylanase, cellulase, β-glucanase, amylase, and β-mannanase are all food-grade enzyme preparations;
[0138] The preparation method of described bacillus subtilis culture comprises the steps:
[0139] (1) Activation of strains
[0140] The well-preserved slant strain of Bacillus subtilis 1398-2-12 was inoculated on the slant medium, cultured at 36°C for 36 hours to activate the strain, and activated 3 times in this way;
[0141] The slant medium consists of: beef extract 10g, sodium chloride 12g, peptone 20g, glucose 5g, (...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com