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Neutral protease-containing growing pig dedicated enzyme and preparation method thereof

A technology of neutral protease and acid protease, which is applied in the field of enzyme preparation, can solve the problems of unmeasured and complex structure, and achieve the effect of wide applicable pH range and high temperature tolerance

Active Publication Date: 2015-04-01
SHANXI DAYU BIOLOGICAL ENG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main reason is that the structure of the market is relatively complex, the market is diversified, and it is even impossible to measure

Method used

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  • Neutral protease-containing growing pig dedicated enzyme and preparation method thereof

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Comparison scheme
Effect test

Embodiment 1

[0057] A special enzyme for growing pigs containing neutral protease, consisting of the following enzyme preparations in parts by weight:

[0058] Bacillus subtilis culture 35 parts, acid xylanase 40 parts, acid protease 25 parts, phytase 25 parts, beta-glucanase 17 parts, cellulase 12 parts, amylase 12 parts, beta-mannose 12 parts of glycanase, 8 parts of pectinase, 12 parts of Chinese herbal medicine extract, 12 parts of protective agent, 12 parts of activator.

[0059] The acid xylanase, acid protease, phytase, β-glucanase, cellulase, amylase, β-mannanase, and pectinase are all food-grade enzyme preparations;

[0060] The preparation method of described bacillus subtilis culture comprises the steps:

[0061] (1) Inoculate the well-preserved slant strain of Bacillus subtilis 1398-2-12 on the slant medium, culture at 30°C for 24 hours to activate the strain, and activate it twice;

[0062] The slant medium consists of: beef extract 3g, sodium chloride 5g, peptone 10g, gluco...

Embodiment 2

[0096] A special enzyme for growing pigs containing neutral protease, consisting of the following enzyme preparations in parts by weight:

[0097]Bacillus subtilis culture 30 parts, acid xylanase 30 parts, acid protease 20 parts, phytase 20 parts, β-glucanase 15 parts, cellulase 10 parts, amylase 10 parts, β-mannose 10 parts of glycanase, 5 parts of pectinase, 10 parts of Chinese herbal medicine extract, 10 parts of protective agent, 10 parts of activator.

[0098] The acid xylanase, acid protease, phytase, β-glucanase, cellulase, amylase, β-mannanase, and pectinase are all food-grade enzyme preparations;

[0099] The preparation method of described bacillus subtilis culture comprises the steps:

[0100] (1) Activation of strains

[0101] The well-preserved slant strain of Bacillus subtilis 1398-2-12 was inoculated on the slant medium, cultured at 33°C for 30 hours to activate the strain, and activated twice in this way;

[0102] The slant medium consists of: beef extract 6...

Embodiment 3

[0135] A special enzyme for growing pigs containing neutral protease, consisting of the following enzyme preparations in parts by weight:

[0136] Bacillus subtilis culture 40 parts, acid xylanase 50 parts, acid protease 30 parts, phytase 30 parts, β-glucanase 20 parts, cellulase 15 parts, amylase 15 parts, β-mannose 15 parts of polycanase, 10 parts of pectinase, 15 parts of Chinese herbal medicine extract, 15 parts of protective agent, 15 parts of activator.

[0137] The acid xylanase, acid protease, phytase, β-glucanase, cellulase, amylase, β-mannanase, and pectinase are all food-grade enzyme preparations;

[0138] The preparation method of described bacillus subtilis culture comprises the steps:

[0139] (1) Activation of strains

[0140] The well-preserved slant strain of Bacillus subtilis 1398-2-12 was inoculated on the slant medium, cultured at 36°C for 36 hours to activate the strain, and activated 3 times in this way;

[0141] The slant medium consists of: beef extr...

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Abstract

The invention discloses a neutral protease-containing growing pig dedicated enzyme and a preparation method thereof and belongs to the technical field of zymin preparation. The neutral protease-containing growing pig dedicated enzyme is scientifically compounded from bacillus subtilis culture, acidic xylanase, acid protease, phytase, beta-dextranase, cellulase, amylase, beta-mannase, pectinase, Chinese herb extracts, a protective agent and an activating agent. The bacillus subtilis culture can improve immunity of the animal organism, promote development of immune organs and maturity of intestinal structures and functions of animals, and increase daily gain of animals and improve feed conversion ratio. The neutral protease-containing growing pig dedicated enzyme provides a safe digestive enzyme for livestock, effectively relieves digestion burden, increases the utilization rate of raw materials and the growth rate and protects the environment. In addition, appropriate amount of activating agent can fully play the efficiency of a zymin under the same condition and saves the additive amount of the zymin; the Chinese herb extracts are scientifically compounded to prolong the expiration date of a composite zymin and improve the immunity of livestock.

Description

technical field [0001] The invention belongs to the technical field of preparation of enzyme preparations, in particular to a special enzyme for growing pigs containing neutral protease and a preparation method thereof. Background technique [0002] There are four main reasons for adding enzyme preparations to feed: 1. Degradation of anti-nutritional factors present in animal feed. These substances cannot be degraded by animal endogenous enzymes, thereby interfering with the normal metabolism of animals, resulting in indigestion of animals and decreased production performance. 2. Improve the utilization rate of starch, protein and minerals. These substances are either surrounded by fiber-rich cell walls, or are present in certain structures that cannot be digested by animals (for example, in plant feed materials, a large amount of phosphorus is present in the form of phytate phosphorus.). 3. Degrade some specific chemical bonds in raw materials. These chemical bonds canno...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/42C12N9/16C12N9/26C12N9/50C12N9/18C12N9/88C12N9/44C12N9/02C12N9/04A23K1/165
CPCA23K20/00A23K20/189A23K50/30C12N9/0006C12N9/0089C12N9/16C12N9/18C12N9/2402C12N9/2411C12N9/2425C12N9/2437C12N9/244C12N9/2445C12N9/2457C12N9/248C12N9/2491C12N9/50C12N9/88C12Y101/03004C12Y115/01001C12Y301/03002C12Y302/01002C12Y302/01021C12Y302/01041
Inventor 李海清胡永明胡波封章平王永兰
Owner SHANXI DAYU BIOLOGICAL ENG CO LTD
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