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Method of reducing cadmium ions by M13 phage-mediated nano-iron

A bacteriophage and cadmium ion technology, which is applied in the fields of nanomaterial preparation and environmental pollution control, can solve the problems of low stability and easy agglomeration of nanometer iron, and achieves the effects of rapid reaction, improved reduction rate and good industrial application prospects.

Active Publication Date: 2014-06-25
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, nano-iron is very easy to agglomerate during the preparation process, and its stability is not high. Therefore, how to solve the problem of agglomeration of nano-zero-valent iron in the preparation process will become the key to the success of this method.

Method used

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  • Method of reducing cadmium ions by M13 phage-mediated nano-iron
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  • Method of reducing cadmium ions by M13 phage-mediated nano-iron

Examples

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Effect test

Embodiment 1

[0031] Example 1 Synthesis of zero-valent iron nanoparticles mediated by iron-adsorption-specific M13 phage

[0032] Amplification of iron-adsorbing specific M13 phage: M13 Phage Dodeceptide Display Peptide Library Kit (Ph.D.-12 TM Phage Display Peptide Library Kit) and Heptapeptide Display Peptide Library Kit (Ph.D.-7 TM Phage Display Peptide Library Kit) was purchased from NEB Corporation. Among them, dodecapeptide and heptapeptide are expressed at the N-terminus of pIII protein with 5 copies at one end of M13 phage, and the complexity is ~2.7×10 9a transformant. The N-terminal sequence of the pVIII protein of 2700 copies of each phage and wild-type phage in the peptide library is N-Ala-Glu-Gly-Asp-Asp-Pro-Ala.... Previous studies have shown that these M13 phages have the ability to specifically adsorb iron cations (Ling T, et al. Virus-mediated FCC Iron Nanoparticle Induced Synthesis of Uranium Dioxide Nanocrystal. Nanotechnology, 2008, 19(11): 115608-115613), saying ...

Embodiment 2

[0035] Example 2 Cadmium Adsorption Specific Genetic Engineering Phage M13 (Cd-s) Construction

[0036] 7-peptide panning with cadmium adsorption specificity: Use the M13 phage pIII protein heptapeptide display peptide library kit (Ph.D.-7TM Phage Display Peptide Library Kit) purchased from NEB Company to perform cadmium adsorption-specific peptide panning select. Cadmium metal blocks were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai). Put the small cadmium metal pieces in a small centrifuge tube containing 200 μl of TBST buffer containing 0.1% Tween-20, add 10 μl of the original peptide library of 7 peptides, and shake and combine for 60 minutes. Pour off the buffer, and use 200 μl of 0.1% Tween-20 TBST buffer to wash the metal block 10 times. Only the phage with strong binding force to Cd can remain on the metal block. Then put the metal blocks into 200 μl TBS buffer containing 20 mM Cd ions for competitive elution. The eluted phages were amplified accord...

Embodiment 3

[0049] Example 3M13(Fe-sp)-mediated synthesis of zero-valent nano-iron efficiently reduces M13(Cd-s) dispersed divalent cadmium ions

[0050] As shown in Example 1, M13(Fe-s)-mediated nanometer zero-valent iron was synthesized under normal temperature and pressure conditions. Then take 100 μL prepared CdCl 2 (500μM) aqueous solution, add 100μL methyl xylenol blue (0.125%) aqueous solution to it, after the color is stable, add about 10 11 M13 (Cd-s) in pfu, mix well to get M13 (Cd-s) dispersed divalent cadmium ions. Using M13(Fe-s)-mediated synthesis of zero-valent nano-iron as a reducing agent, the reduction reaction of M13(Cd-s) dispersed cadmium ions was carried out under normal temperature and pressure. After the two are fully mixed in the cuvette of the spectrophotometer, the absorbance is measured at 605nm immediately. The change in absorbance is produced by the color change of methylxylenol blue, which indicates the content of cadmium ions remaining in the solution. T...

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Abstract

The invention discloses a method of reducing cadmium ions by M13 phage-mediated nano-iron and belongs to the field of environmental pollution governance and nano material preparation. According to the method, iron-adsorbed special M13 phage is adopted to synthesize uniformly dispersed zero-valent nano-iron particles in a mediated manner, and adopts cadmium-adsorbed special M13 phage M13 (Cd-s) to uniformly disperse divalent cadmium ions; then, the zero-valent nano-iron mediated and dispersed by the M13 phage is taken as a reducing agent so as to efficiently reduce divalent cadmium ions uniformly dispersed on the surface of M13(Cd-s). The method disclosed by the invention is high in reducing efficiency, quick in reaction, capable of efficiently catalyzing the divalent ions to be reduced into zero-valent cadmium on the phage surface, so that cadnimum pollution in environment is removed, and nano-particles of zero-valent iron, iron oxide and zero-valent cadmium also can be quickly prepared.

Description

technical field [0001] The invention belongs to the technical field of nanomaterial preparation and environmental pollution control, and specifically relates to a method for reducing cadmium ions by nanometer iron mediated by M13 bacteriophage. Background technique [0002] Cadmium (cadmium, Cd) is widely used in electroplating, batteries, automobiles, aviation, pigments, paints, printing, plastics industries and other industries. The common valence states of cadmium in the environment are mainly two valence and zero valence. For most organisms, cadmium and its compounds have a certain degree of toxicity: cadmium has a stimulating effect on the respiratory tract, and long-term exposure can cause loss of smell and macular plaques on the gums; cadmium compounds are not easily absorbed by the intestinal tract, but can be absorbed by the human body through breathing. Absorption and accumulation in the liver or kidney cause harm; cadmium can also cause osteoporosis and softening...

Claims

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Application Information

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IPC IPC(8): C12P3/00C12N7/01C12N15/63
Inventor 于慧敏张帅中野和彦沈忠耀
Owner TSINGHUA UNIV
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