Purpureo cilliumsp with high resistance on cadmium, and extracting method and application thereof

An extraction method, the technology of Porphyrospora, is applied in the field of screening of cadmium-resistant strains, which can solve the problems of removing heavy metal obstacles and difficulty in timely removal, and achieves the effect of low cost, simple and convenient separation and purification method, and strong cadmium resistance

Active Publication Date: 2018-01-19
中科厚土润泽环境科技(江苏)股份有限公司
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Taking the pollution of cultivated land in China as an example, most of the pollution is non-point source pollution and it is difficult to remove it in ti

Method used

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  • Purpureo cilliumsp with high resistance on cadmium, and extracting method and application thereof
  • Purpureo cilliumsp with high resistance on cadmium, and extracting method and application thereof
  • Purpureo cilliumsp with high resistance on cadmium, and extracting method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The isolation of embodiment 1 bacterial strain

[0024] The soil samples for this experiment were collected at the Luancheng Experimental Station of the Chinese Academy of Sciences. The geographical location is 37°53' north latitude and 114°41' east longitude. It belongs to the warm temperate semi-humid monsoon climate. The soil type is mainly moist cinnamon, which represents the soil type in the northern part of the North China Plain. . The annual average temperature is 12.2°C and the annual average precipitation is 530mm. The cultivated soil at 3 points was collected for mixed sample processing.

[0025] Add 5mL of 16mM CdCl to each 50mL centrifuge tube containing 20g of soil samples in advance 2 solution, at room temperature for 21 days. Get 5g of treated soil sample to make soil suspension, press 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 A 10-fold ratio dilution was performed. Spread 200 μL of the diluted soil suspension evenly in the solution containing 16 mM CdCl...

Embodiment 2

[0027] Example 2 Morphological Identification

[0028] 1. Colony Morphological Observation

[0029] Insert the strain into the Martin's medium plate, place it at 28°C for continuous culture for 12 days, and observe the morphological characteristics of the colony. The results are shown in figure 1 .

[0030] Such as figure 1 As stated, the colony has a bacterial ring, the hyphae are white, and the texture is velvet. A large number of conidia structures are produced, and the surface color of the conidia is light pink gray; there is no exudate on the surface.

[0031] 2. Microscope morphology observation

[0032] Use a dissecting needle to pick out a little colony from the colony cultured on the Martin's medium plate, put it on a glass slide dripped with lactic acid phenol cotton blue staining solution in the middle, and then use two dissecting needles to disperse the colony as much as possible and mix with lactic acid phenol Mix the cotton blue staining solution evenly, add ...

Embodiment 3

[0040] Example 3 Cadmium tolerance and cadmium adsorption experiments

[0041] Since the test strains were screened from 16mM CdCl 2 Pretreated soil and purified in 16mM CdCl 2 Concentration of the Martin medium plate, so the strain can be 16mM CdCl 2 Grows well under stress.

[0042] Punch a hole on the edge of a well-growing colony with a diameter of 0.7 cm, and inoculate the solid medium block containing mycelia that was taken out of the hole into 16mM CdCl 2 Concentration of the Martin medium plate, record the mycelial diameter every day, the results are shown in Figure 5 .

[0043] Depend on Figure 5 It can be seen that the diameter of mycelia in the cadmium-free and cadmium-containing medium all showed a gradual increase trend. 2 The diameter of mycelium in the Martin's medium with high concentration was not very different from that of the strain without cadmium, which indicated that the strain had a strong tolerance to cadmium.

[0044] Punch a hole on the edge...

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Abstract

The invention provides a purpureo cilliumsp with high resistance on cadmium, and an extracting method and application thereof. The bacterial strain belongs to purpureo cilliumsp, and is registered andpreserved in CGMCC (China General Microbiological Culture Collection Center) on September 12th, 2017; the preservation number is CGMCC No.14159; the ITS sequence is shown as SEQ.ID.NO:1. The bacterial strain has a cadmium adsorption function; the cadmium in soil can be adsorbed; the absorption of plants on the cadmium is reduced, so that the plant growth is promoted.

Description

technical field [0001] The invention belongs to the technical field of screening of cadmium-resistant strains, and in particular relates to a purple sporophyte with high resistance to cadmium, an extraction method and application thereof. Background technique [0002] Official documents show that our arable land has polluted more than 19.4% of the total arable land, and cadmium is the most widely polluted pollutant, accounting for about 7% of the total arable land. At the same time, under the general background of "every river is polluted", cadmium pollution in various water bodies including natural rivers, lakes, urban sewage rivers and industrial sewage seepage pits cannot be ignored. The ecological restoration of cadmium in cultivated land and industrial sewage has become a worldwide problem. Taking the pollution of cultivated land in China as an example, most of the pollution is non-point source pollution and it is difficult to remove it in time. The fallow restoration ...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N1/02B09C1/10C02F3/34C12R1/645
Inventor 李小方郑鑫
Owner 中科厚土润泽环境科技(江苏)股份有限公司
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