Penicillium oxalicum strain capable of degrading cellulose and adsorbing cadmium and application thereof

A technology for Penicillium oxalate and cellulose degradation, which is applied in the field of environmental management, can solve problems such as reducing the effectiveness of lead, and achieve the effects of reducing bioavailability, repairing heavy metal pollution in soil, and improving colonization ability.

Pending Publication Date: 2019-10-18
湖南慧谷农业生态研究院有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Chinese patent CN 105296363A discloses a Penicillium oxalicum NJDL-03, which can significantly increase the solubility of hydroxyapatite, and at the same time release phosphate ions that can precipitate lead ions in the environment and reduce the effectiveness of lead in the environment; Chinese patent CN 106047730A discloses a Penicillium oxalicum strain EU2101, the leaching solution of the bacteria after solid fermentation and cultivation has cellulase activity, which can efficiently hydrolyze cassava residues pretreated by alkali; Report on Penicillium oxalicum with ability to adsorb heavy metal cadmium on cellulose

Method used

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  • Penicillium oxalicum strain capable of degrading cellulose and adsorbing cadmium and application thereof
  • Penicillium oxalicum strain capable of degrading cellulose and adsorbing cadmium and application thereof
  • Penicillium oxalicum strain capable of degrading cellulose and adsorbing cadmium and application thereof

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Experimental program
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Effect test

Embodiment 1

[0028] Embodiment 1: the culture medium that invention uses

[0029] (1) Separation medium: CMC medium: sodium carboxymethylcellulose (CMC-Na) 10.0g, KH2PO4 0.25g, potato juice 100mL, agar powder 18.0g, distilled water 1000mL.

[0030] (2) Purification and storage medium: PDA medium: 200 g of potatoes, 20.0 g of glucose, 18.0 g of agar powder, and 1000 mL of distilled water.

[0031] (3) Cadmium ion-containing medium: 200g of potatoes, 20.0g of glucose, 18.0g of agar powder, and 1000mL of distilled water. Add cadmium ion-containing mother liquor (20mg / mL) as needed to prepare solid medium with different concentrations of cadmium ions.

[0032] (4) Screening medium:

[0033] a. Congo red medium: CMC-Na 2.0g, KH 2 PO 4 0.5g, MgSO 4 ·7H 2 O 0.25g, (NH 4 ) 2 SO 4 1.0g, Congo red 0.1g, agar powder 18.0g, distilled water 1000mL;

[0034] b. Re-screening medium: CMC-Na 5.0g, KH 2 PO 4 1.0g, MgSO 4 ·7H 2 O 0.5g, NaNO 3 3.0g, KCl0.5g, FeCl 3 ·6H 2 O 0.01g, distilled w...

Embodiment 2

[0036] Embodiment 2: Separation and purification of bacterial strain

[0037] The specific method for the isolation and purification of the bacterial strain is: using the dilution plate coating method, the soil sample is prepared by gradient dilution with sterile water for 10 -3 -10 -5 Bacteria suspension, and spread on the separation medium, and cultured in a constant temperature incubator at 28°C for 2-3 days, the isolated fungi were streaked, purified and preserved in PDA medium, and the purified strains were Store in a refrigerator at 4°C.

[0038] The classification of the Penicillium oxalicum strain is named Penicillium oxalicum (Penicillium oxalicum) WAF6, and its preservation number is ACCC 32731, and its biological characteristics are: the edge of the colony is white and velvety; the surface is covered with dark green spores, and the spores are easy to fall off; The optimum culture pH is 6.5, and the optimum culture temperature is 28°C; spores can be produced after ...

Embodiment 3

[0039] Example 3: Evaluation of cadmium ion tolerance of bacterial strain WAF6

[0040] The cadmium ion tolerance evaluation method of bacterial strain WAF6 of the present invention specifically comprises the following steps:

[0041] (1) Prepare PDA medium containing different cadmium ion concentrations, the concentration is set to 0, 10, 50, 80, 100, 150, 200 mg / L;

[0042] (2) Use a 5mm hole puncher to make bacterial cakes from the bacterial strain, inoculate it on the plate medium described in step (1) under aseptic conditions, and cultivate it in a constant temperature incubator at 26-28°C for 3-5 days to observe the growth of the bacterial strain. growing situation.

[0043] Strain WAF6 grows well on a plate with a cadmium ion concentration of 0-150mg / L, but growth is completely inhibited on a plate with a cadmium ion concentration of 200mg / L, so the cadmium ion concentration tolerance range of this strain is 0-150mg / L.

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Abstract

The invention provides a penicillium oxalicum strain capable of degrading cellulose and adsorbing cadmium. The penicillium oxalicum strain is classified and named as Penicilliumoxalicum WAF6, and thepreservation number of the penicillium oxalicum strain is ACCC 32731. The cadmium ion concentration tolerance range of the penicillium oxalicum strain is 0-150 mg/L; the penicillium oxalicum strain can produce cellulase, has a good degradation effect on rice straw, and is basically degraded on the 15th day when applied to degradation of the rice straw; meanwhile, the strain has the capacity to absorb heavy metal cadmium, and the penicillium oxalicum strain has a cadmium adsorption rate greater than 76% on the 30th day when applied to the treatment of cadmium-contaminated soil. The strain is applied to the bioremediation of a cadmium-contaminated rice field, and can achieve an effect of restoring the soil metal cadmium contamination while degrading the rice straw.

Description

technical field [0001] The invention relates to the technical field of environmental treatment, in particular to a Penicillium oxalic acid strain capable of degrading cellulose and adsorbing cadmium and an application thereof. Background technique [0002] China is one of the countries with the most abundant straw resources. According to reports, the annual output of crop straw in my country is 600-700 million tons, accounting for about 1 / 3 of the world's total straw production, and the utilization space of straw resources is relatively large. Stress matters. At present, the methods for straw degradation mainly include direct returning to the field, physical degradation, chemical degradation, and biological degradation. Compared with other methods, the biodegradation method has the advantages of mild treatment, low energy consumption, and no secondary pollution to the environment. And received much attention. [0003] The World Health Organization (WHO) lists cadmium as a ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14B09C1/10C12R1/80
CPCC12N1/14B09C1/105C12R2001/80C12N1/145
Inventor 谭石勇张冬雪文亚雄刘备罗志威
Owner 湖南慧谷农业生态研究院有限公司
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