Cadmium-tolerant intestinal strain and cadmium adsorption method

A technology of enterobacteriaceae and tolerance, applied in the field of microorganisms, can solve the problems of large environmental impact, poor system stability, high cost, etc., and achieve the effect of optimizing adsorption characteristics and adsorption conditions

Active Publication Date: 2019-01-25
GUANGXI NORMAL UNIV
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Problems solved by technology

These methods often have high technical requirements and high cost, and are not suitable for large-scale contaminated soil remediation.
Chemical methods, such as adding zeo

Method used

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  • Cadmium-tolerant intestinal strain and cadmium adsorption method
  • Cadmium-tolerant intestinal strain and cadmium adsorption method
  • Cadmium-tolerant intestinal strain and cadmium adsorption method

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Embodiment 1

[0028] Part I: Acquisition of Enterobacter sp. FM-1

[0029] 1. Acquisition of Enterobacteriaceae

[0030] (1) Soil: The soil was collected from the surface soil of the Ninghe Manganese Mine mining area in Hezhou, Guangxi Zhuang Autonomous Region. The specific steps are as follows: use random sampling method, take 20 soil samples of 0-10 cm with soil auger, take them back to the laboratory, air dry, mix them evenly, pass through a 1 mm sieve, and set aside.

[0031] (2) Preliminary extraction night preparation: take 10g of the above (1) soil, put it into a triangular flask containing 90ml of sterile water under aseptic operation, and shake the incubator in a 30°C incubator for 10 minutes to prepare the soil primary Extraction.

[0032] (3) Strain enrichment: Take 1.0ml of the above-mentioned (2) soil for initial extraction, inoculate in 50ml LB liquid medium, incubate at 30°C for 150r min -1 The enriched culture was obtained by shaking culture for 5 days. The enrichment me...

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Abstract

The invention discloses an Enterobacter sp. FM-1 obtained through separation from mining soil, purifying and domestication, deposited at China Typical Culture Collection on December22, 2017, the deposit number of which is CCTCC M2017825. The method comprises such steps as taking soil, culturing soil, obtaining initial extracting solution, enriching culture with LB liquid medium, purifying culturewith LB solid medium, inoculating single colony into LB plate medium containing cadmium for further domestication and culture, and obtaining Enterobacter sp. FM-1 with high tolerance to cadmium. The Enterobacter sp. FM-1 has good tolerance and adsorption properties to cadmium. The adsorption of cadmium in the medium containing 4 mmol L-1 cadmium. The maximum adsorption rate of cadmium was 80.36% at 30 DEG C and pH 7. 190.45 mg g-1 of cadmium adsorption capacity was added under sterile operation at this time. The strain can be applied to the remediation of cadmium polluted water and soil with phytoremediation, and has a wide application prospect.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to an enterobacter and a method for adsorbing cadmium. Background technique [0002] my country's soil is seriously polluted by heavy metals, and the land with cadmium as the main pollutant accounts for about half. The methods of remediation of cadmium-contaminated soil mainly include physical methods, such as soil washing method, soil replacement method, electrodynamic treatment method, vitrification and so on. These methods often have high technical requirements and high costs, and are not suitable for large-scale contaminated soil remediation. Chemical methods, such as adding zeolite, lime, fly ash, domestic garbage, etc.; these methods often have the characteristics of large impact on the environment, poor removal effect, and poor system stability. The biological method has attracted much attention because it is environmentally friendly, does not produce secondary pollu...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/02C12N1/04C12N1/36C12R1/01
CPCC12N1/02C12N1/04C12N1/20C12N1/36C12N1/205C12R2001/01
Inventor 于方明刘可慧周振明李艺陈朝述
Owner GUANGXI NORMAL UNIV
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