Cadmium-tolerant intestinal strain and cadmium adsorption method
A technology of enterobacteriaceae and tolerance, applied in the field of microorganisms, can solve the problems of large environmental impact, poor system stability, high cost, etc., and achieve the effect of optimizing adsorption characteristics and adsorption conditions
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[0028] Part I: Acquisition of Enterobacter sp. FM-1
[0029] 1. Acquisition of Enterobacteriaceae
[0030] (1) Soil: The soil was collected from the surface soil of the Ninghe Manganese Mine mining area in Hezhou, Guangxi Zhuang Autonomous Region. The specific steps are as follows: use random sampling method, take 20 soil samples of 0-10 cm with soil auger, take them back to the laboratory, air dry, mix them evenly, pass through a 1 mm sieve, and set aside.
[0031] (2) Preliminary extraction night preparation: take 10g of the above (1) soil, put it into a triangular flask containing 90ml of sterile water under aseptic operation, and shake the incubator in a 30°C incubator for 10 minutes to prepare the soil primary Extraction.
[0032] (3) Strain enrichment: Take 1.0ml of the above-mentioned (2) soil for initial extraction, inoculate in 50ml LB liquid medium, incubate at 30°C for 150r min -1 The enriched culture was obtained by shaking culture for 5 days. The enrichment me...
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