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Preparation method of microbial cadmium removal absorbent and application of absorbent in cadmium removal of powder food

A technology of microorganisms and adsorbents, applied in the application of cadmium removal from powder food, the field of preparation of microbial cadmium removal adsorbents, can solve the problems such as the difficulty of separating microbial cadmium removal adsorbents from powdery raw materials, and achieve easy separation and reduce Effect of cadmium content

Inactive Publication Date: 2018-08-31
WUHAN POLYTECHNIC UNIVERSITY
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Problems solved by technology

[0004] The main purpose of the present invention is to propose a preparation method of microbial cadmium removal adsorbent and its application in powder food cadmium removal, aiming to solve the problem of difficult separation of microbial cadmium removal adsorbent and powdery raw materials

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  • Preparation method of microbial cadmium removal absorbent and application of absorbent in cadmium removal of powder food
  • Preparation method of microbial cadmium removal absorbent and application of absorbent in cadmium removal of powder food
  • Preparation method of microbial cadmium removal absorbent and application of absorbent in cadmium removal of powder food

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preparation example Construction

[0035] The present invention proposes a preparation method of a microbial cadmium removal adsorbent, figure 1 It is an embodiment of the preparation method of the microbial cadmium removal adsorbent provided by the present invention. see figure 1 , the preparation method of the microbial cadmium removal adsorbent comprises the following steps:

[0036] Step S10, centrifuging the Lactobacillus plantarum liquid and diluting it with water to form a Lactobacillus plantarum suspension;

[0037] In this embodiment, step S10 specifically includes: centrifuging the Lactobacillus plantarum cultured to the logarithmic phase at a speed of 4000-6000 r / min for 8-12 minutes, removing the supernatant, and taking sterile water for colony precipitation Dilute to OD 620 (The absorbance value at 620 nm) was 1.5, and a suspension of Lactobacillus plantarum was obtained. Wherein, the Lactobacillus plantarum liquid can be obtained by cultivating the Lactobacillus plantarum to the logarithmic ph...

Embodiment 1

[0055] (1) Centrifuge the Lactobacillus plantarum cultured to the logarithmic phase at 4000r / min for 12min, remove the supernatant, take the colony precipitate and add sterile water to dilute to OD 620 is 1.5, obtains plantarum lactobacillus suspension;

[0056] (2) Dissolve 2g of polyvinyl alcohol and 3g of sodium alginate in 84g of distilled water by heating, sterilize at 121°C for 30min, cool to 37.5°C, and then add 10g of Lactobacillus plantarum suspension prepared in step (1) and 1g of silicon Alginate, stir well to form a mixed solution;

[0057] (3) Use a 1mL syringe to draw the mixed solution, and drop it into 4°C, 4% CaCl 2 solution, cross-linked at 4°C for 24h to form microbial pellets, filtered from CaCl 2 Microbe pellets are separated from the solution and washed three times with sterile water to prepare a microbial cadmium-removing adsorbent.

Embodiment 2

[0059] (1) Centrifuge the Lactobacillus plantarum cultured to the logarithmic phase at 5000r / min for 10min, remove the supernatant, take the colony precipitate and add sterile water to dilute to OD 620 is 1.5, obtains plantarum lactobacillus suspension;

[0060] (2) Dissolve 1.5g of polyvinyl alcohol and 2.5g of sodium alginate in 83.8g of distilled water by heating, sterilize at 120°C for 33min, cool to 36.5°C, and then add 11g of Lactobacillus plantarum suspension prepared in step (1) and 1.2g of diatomaceous earth, after being stirred evenly, a mixed solution is formed;

[0061] (3) Draw up the mixed solution with a 1mL syringe, and add 3.5% CaCl at 3.5°C drop by drop. 2 solution, cross-linked at 3.5°C for 26h to form microbial pellets, filtered from CaCl 2 Microbe pellets are separated from the solution and washed three times with sterile water to prepare a microbial cadmium-removing adsorbent.

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Abstract

The invention discloses a preparation method of a microbial cadmium removal absorbent and an application of the absorbent in cadmium removal of the powder food. The preparation method of the microbialcadmium removal absorbent comprises the following steps of: diluting a lactobacillus plantarum solution with water after centrifugation to form a lactobacillus plantarum suspension, dissolving polyvinyl alcohol and sodium alginate in distilled water, adding the lactobacillus plantarum suspension and kieselguhr for stirring to form a mixed solution, adding the mixed solution into a CaCl2 solutionfor cross-linking reaction to form a microbial pellet, and separating the microbial pellet from the CaCl2 solution to form the microbial cadmium removal absorbent. When the microbial cadmium removal absorbent is applied to cadmium removal treatment of the powder food with the excessive cadmium, the heavy metal cadmium in a raw material is absorbed by acid production capability and chemical structures of microorganisms and good absorbability of the kieselguhr; a cadmium content in the powder food with the excessive cadmium can be reduced significantly; and the cadmium is easy to separate.

Description

technical field [0001] The invention relates to the technical field of food processing, in particular to a preparation method of a microbial cadmium removal adsorbent and its application in powder food cadmium removal. Background technique [0002] For foods with excessive heavy metals, such as rice, corn, soybeans and other foods with excessive heavy metal cadmium, it is necessary to remove cadmium from the food to reduce the cadmium content to meet the edible standard. The following uses rice with excessive cadmium as an example to illustrate. [0003] At present, the methods used to reduce cadmium in rice with cadmium exceeding the standard mainly include finishing, deep processing, water immersion, pickling and microbial adsorption. Among them, the reduction rate of cadmium in finishing processing is too low, and the time and energy required for deep processing Excessive consumption and high cost, the water immersion method has the disadvantages of low cadmium reduction ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C12N11/10C12N11/04A23L5/20C12R1/25
CPCA23L5/273A23L5/28C12N11/04C12N11/10C12N11/14A23V2400/169
Inventor 宫智勇陈雨薇王蕾吴永宁
Owner WUHAN POLYTECHNIC UNIVERSITY
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