High-cadmium-adsorption filamentous fungi hair mold XLC as well as preparation method and application thereof

A technology of filamentous fungus and mucormyces, applied in the field of preparation of filamentous fungus mucormyces, can solve problems such as difficult to popularize and apply, difficult to play a role, cure the symptoms but not the root cause, and achieve high application value, high ability to absorb heavy metals, and high efficiency Adsorption effect

Active Publication Date: 2013-12-18
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] For heavy metal pollution, traditional methods include engineering treatment measures, physical and chemical restoration, and chemical restoration measures, including ion exchange, adsorption, electrochemical treatment, extraction, and membrane separation (Wang, Wang, 2009). From a practical point of view, it is found that engineering treatment measures are still only palliatives but not the root cause in the case of spending a lot of manpower and money; physical and chemical remediation methods are only suitable for small-scale pollution remediation (David et al., 1995); the effect of chemical remediation has been proved to be relatively good Effects on the adsorption, redox, antagonism or precipitation of heavy metals to finally reduce heavy metal pollution, and it is a method that is widely used in pollution control (Naidu et al., 1997)
However, practice has shown that although some of the above-mentioned remediation methods can reduce the bioavailability of heavy metals in the environment to a certain extent, they all have their own defects and limitations. -1 ) environment, it is difficult to play a role, the cost is huge, the effect is not good, and it is difficult to promote the application

Method used

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  • High-cadmium-adsorption filamentous fungi hair mold XLC as well as preparation method and application thereof
  • High-cadmium-adsorption filamentous fungi hair mold XLC as well as preparation method and application thereof
  • High-cadmium-adsorption filamentous fungi hair mold XLC as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] A preparation method of the filamentous fungus Mucor XLC with high adsorption of cadmium, the steps are:

[0050] A. Weigh the content of heavy metal cadmium to be 50mg·Kg -1 10g potted test soil sample for heavy metal repair in 90ml sterile water with 5 0.5mm glass beads, 28°C constant temperature shaker 150r min -1 After shaking for 15 minutes and standing still for 1 minute, the supernatant was taken for 10-fold serial dilution.

[0051] B. Spread 100 μL of dilutions of different dilutions on the Martin solid medium containing 60 mM cadmium, let it rest for a while, and place it upside down in a constant temperature incubator at 28°C for 5 days.

[0052] C. Separating and purifying the microorganisms grown on the culture medium by streaking and re-screening for verification. After 10 times of separation and screening, save the bacterial strains with stable resistance to cadmium and store them on the PDA slope for future use.

[0053] The applicant named the isolate...

Embodiment 2

[0064] The application of a filamentous fungus Mucor with high adsorption of cadmium in the bioremediation of heavy metal cadmium polluted water and soil, the steps are:

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Abstract

The invention discloses high-cadmium-adsorption filamentous fungi hair mold XLC as well as a preparation method and an application thereof. The preparation method comprises the following steps of: weighing and adding pot experiment soil sample containing 50mg.Kg<-1> of heavy metal cadmium for heavy metal restoration in sterile water added with glass beads, vibrating on a constant-temperature shaking table, standing, taking supernatant liquid and diluting; coating different dilution factors of diluent on a Martin solid culture medium containing cadmium, and inversely placing the flat plate in a constant-temperature incubator for cultivation; carrying out streaking and purification on microorganisms which grow on the culture medium, carrying out secondary screening and verification, thus obtaining the filamentous fungi hair mold XLC bacterial strain having stable resistance capacity on cadmium after isolation and screening, and storing on a PDA (Potato Dextrose Agar) inclined plane for standby application. The preservation number of the bacterial strain is CCTCC NO: M2012136. The cadmium adsorption capacity of the active thallus of the bacterial strain is higher than that of a dead thallus. The bacterial strain has high-efficiency removal capacity on cadmium in electroplating wastewater treatment and can be used for repairing the cadmium pollution in water and soil in practical application.

Description

technical field [0001] The invention belongs to the field of water body environment treatment, and specifically relates to a filamentous fungus Mucor with high adsorption of cadmium, and also relates to a preparation method of the filamentous fungus Mucor with high adsorption of cadmium, and also relates to a filamentous fungus with high adsorption of cadmium. Application of fungus Mucor in bioremediation of heavy metal cadmium polluted water and soil. Background technique [0002] Soil and water are both important components of the natural environment and resources that humans depend on for survival. With the intensification of human activities, more and more heavy metal pollutants have been released through atmospheric deposition, sewage irrigation, solid waste discharge, and pesticides and fertilizers. Applied and other ways into the soil and water (Li Tianjie, 1995). The accumulation of harmful heavy metals to a certain extent will cause toxicity to the ecosystem, and c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14B01J20/28C02F1/28C02F1/62B09C1/10C12R1/645
Inventor 陈雯莉黄巧云朱伟夏璐徐兴健张哲轶
Owner HUAZHONG AGRI UNIV
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