Method for extracting and purifying steviol glycoside from stevia rebaudiana aqueous extract by virtue of series resin column method

A technology of steviol glycosides and stevia water, applied in the field of extraction and purification of steviol glycosides and steviol glycosides, can solve the problems of large waste, high regeneration consumption and high energy consumption, and achieves reduction of three waste discharges, easy resin regeneration, and economic benefits. obvious effect

Active Publication Date: 2012-12-12
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantages of this process are: (1) Adding flocculant introduces new impurities, which need to be removed in subsequent processes, and the precipitate will adsorb a part of steviol glycosides, reducing the yield
(2) The regeneration of desalting and decolorizing resin consumes a lot of acid, alkali and water
In addition, the Chinese patent application number 200710130867.9 uses membrane separation technology to realize the recycling

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) Preparation of upper column solution: crush the dried stevia leaves to 40 mesh for later use. Weigh 100.00g of stevia leaf powder, put it in a 1L beaker, add 500mL of deionized water for extraction, repeat the extraction twice, combine the filtrate to obtain 1300mL of stevia leaf extract, and obtain a clear aqueous solution through solid-liquid separation , adjust the pH of the solution to 5.0, which is the upper column solution;

[0024] (2) Pretreatment of the macroporous anion-exchange resin packed in the first column: Weigh 200.00g of resin and soak overnight in 250mL of absolute ethanol, pack the resin into a column, wash with deionized water until there is no alcohol smell, and use 250mL of a mass fraction of 4 % sodium hydroxide aqueous solution transforms the resin into OH type, for subsequent use;

[0025] (3) Pretreatment of the macroporous adsorption resin packed in the second column: Weigh 200.00g of resin and soak in 250mL of absolute ethanol overnight...

Embodiment 2

[0030] (1) Preparation of upper column solution: crush the dried stevia leaves to 60 mesh for later use. Weigh 200.00g of stevia leaf powder, put it in a 2L beaker, add 800mL of deionized water for extraction, repeat the extraction twice, combine the filtrate to obtain 2200mL of Stevia rebaudiana water extract, and obtain a clear aqueous solution of Stevia rebaudiana after solid-liquid separation , adjust the pH of the solution to 5.5, which is the upper column solution;

[0031] (2) Removal of water-soluble impurities such as pigments: the supernatant obtained by the processing of step (1) first passes into the pretreated macroporous anion exchange resin column (the first column, the filling resin is D890) with a flow rate of 1.0BV / hour ) for decolorization and impurity removal, the operating temperature is 45°C, the effluent of the first column is directly passed into the pretreated macroporous adsorption resin column (the second column, the resin filled is D101) for adsorpt...

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Abstract

The invention relates to a method for extracting and purifying steviol glycoside from stevia rebaudiana aqueous extract by virtue of a series resin column method and belongs to the technical field of food separation. According to the method, dried leaves of stevia rebaudiana are used as raw materials, and the method comprises the following steps of: crushing the dried leaves of stevia rebaudiana, and adding deionized water into the crushed dried leaves to extract the stevia rebaudiana aqueous extract; filtering to obtain a clear water solution which is an overlying column liquid; removing pigment and soluble impurities of the overlying column liquid by virtue of a macroporous anion exchange resin column, and then directly using a macroporous absorption resin column connected with the macroporous anion exchange resin column to absorb the target product steviol glycoside; after absorption, desorbing the steviol glycoside by using an ethanol water solution, and removing ethanol in the desorption solution to obtain the steviol glycoside water solution with the steviol glycoside purity of more than 92%. The method has the advantages of being short in process flow, simple and convenient in operation and high in steviol glycoside yield, does not need chemical substances such as a flocculating agent in the purification process, and can be used for greatly reducing the emission of three wastes.

Description

technical field [0001] The invention belongs to the technical field of food separation, and relates to a method for purifying steviol glycosides, in particular to a method for directly extracting and purifying steviosides from water extracts of stevia rebaudiana by adopting a series resin column method. Background technique [0002] Steviol glycoside is a non-nutritive high-intensity sweetener extracted from Stevia rebaudiana, a plant of Compositae. Its sweetness is 200-350 times that of sucrose. It has no toxic side effects, does not participate in the metabolism of human tissues, and has stable properties during food processing. . In 2010, the Food and Agriculture Organization of the United Nations and the World Health Organization Joint Expert Committee on Food Additives (JECFA) approved that steviol glycosides with a purity of not less than 95% can be used as food sweeteners. [0003] The traditional process of purifying steviol glycosides includes flocculant removal an...

Claims

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Application Information

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IPC IPC(8): C07H15/256C07H1/08
Inventor 杨瑞金唐乐乐叶发银华霄赵伟张文斌
Owner JIANGNAN UNIV
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