A kind of polypeptide and its application in the preparation of colitis and colitis-related colorectal cancer medicine
A technology for colorectal cancer and colitis, applied in the field of small molecule peptides for the treatment of colitis and colitis-related colorectal cancer
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Embodiment 1
[0046] Example 1: Synthesis of Small Molecular Peptides
[0047] The amino acid sequence of the small molecule polypeptide is shown in SEQ ID NO:1.
[0048] Small molecule peptide synthesis was performed on an ABI 431A solid-phase peptide synthesizer (PE Company, USA). Methods The standard fluorenylmethoxycarbonyl (Fmoc) protocol was used, and arginine was coupled twice. Initially select 0.125mmol p-hydroxymethylphenoxymethyl polystyrene resin (HMP resin), extend the peptide chain from the carboxyl terminal to the amino terminal one by one according to the polypeptide sequence, the amount of each amino acid is 0.5mmol, and the mole of the resin The ratio is 4:1. The α-amino acids of various amino acids are protected by Fmoc, and the other side chain protection groups are: Ser (tBu), Glu (OtBu), Arg (Pmc). The first amino acid is connected to the resin with 4-lutidine, the amino acid is activated with 1-hydroxybenzotriazole and dicycloethyl carbon, and after coupling, the Fmo...
Embodiment 2
[0050] Embodiment 2: the application of small molecular polypeptide in the treatment of mouse colitis, colitis-associated colorectal cancer
[0051] 1. Animals:
[0052] C57BL6 mice, 5 weeks old, 18-20 g, were purchased from the Animal Laboratory of Nantong University, and randomly divided into an experimental group (15 mice) and a control group (15 mice) according to body weight. The experimental group was given AOM / DSS, while the control group was injected with normal saline and drank distilled water freely. No food was fasted during the experiment.
[0053] 2. Drugs:
[0054] Small molecule polypeptide treatment group: 20nmol / kg body weight of the small molecule polypeptide shown in SEQ ID NO: 1 was given for intraperitoneal injection, a total of 6 mice;
[0055] Blank group: intraperitoneal injection of the same volume of PBS as the treatment group, a total of 6 rats.
[0056] 3. Experimental method:
[0057] ①Intraperitoneal injection of AOM / DSS is a common animal mo...
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