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Method for rapid evaluation of variety resistance to bushy stunt of wheat by detecting proliferation rate of virus in coleoptiles

A technology for wheat bush dwarf disease and virus proliferation, which is applied in biochemical equipment and methods, and the determination/inspection of microorganisms, and can solve the problems of short identification period, inaccurate identification results, and long identification period.

Active Publication Date: 2015-07-22
江苏省金地种业科技有限公司
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Problems solved by technology

[0011] The purpose of the present invention is to provide a short identification period and high reliability for the rapid evaluation of the virus proliferation speed in the coleoptile in view of the shortcomings of the current identification method for the resistance of varieties of wheat bush dwarf disease, which has a long identification period and inaccurate identification results. Methods of Wheat Bush Dwarf Variety Resistance

Method used

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  • Method for rapid evaluation of variety resistance to bushy stunt of wheat by detecting proliferation rate of virus in coleoptiles

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Embodiment

[0049] In 2015, the identification method of the invention was used to identify the resistance to bush dwarf disease of some of the collected wheat variety resources. Three wheat varieties, Fu'a 1, Abo and Luyeshu, were respectively set as the identification scales of high resistance, medium resistance and susceptibility. Among them, Fu'a 1 had a disease resistance performance of 51% in the indoor infection identification in 2013-2014. Among the wheat variety resource groups, it ranks 5th, Abo ranks 13th, and green leaf ripeness ranks 26th. The coleoptile collection time points were set as four time points on the 4th, 8th, 12th, and 16th days after the completion of inoculation.

[0050] Real-time fluorescence quantitative PCR obtains the RQ value of each variety cycle sampling time point, as shown in Table 1:

[0051]

[0052] Then draw the RBSDV proliferation index trend line: take the mean RQ value as the ordinate, and the sampling time point after feeding the poison as t...

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Abstract

The invention discloses a method for rapid evaluation of variety resistance to bushy stunt of wheat by detecting proliferation rate of virus in coleoptiles. The method comprises the following steps: 1) selecting identification scale; 2) performing hypoxia induction on wheat to be detected and identifying the coleoptiles of scale variety; 3) inoculating viruliferous laodelphax striatellus into the coleoptiles and then performing dark culture; 4) performing fluorescence quantitative PCR (polymerase chain reaction) to obtain relative expression (RQ value) of RBSDV (rice black streaked dwarf virus) virus S7 and an internal control gene of various wheat varieties at period time points; 5) drawing an RBSDV proliferation index trend line; and 6) evaluating the resistance level and the resistance grade of the wheat variety to be detected to the bushy stunt of the wheat according to the RBSDV proliferation index trend line formula. According to the method disclosed by the invention, the variety resistance to the bushy stunt of the wheat is rapidly evaluated by detecting the proliferation rate of the virus in the coleoptiles, the resistance level and the resistance grade of the wheat variety to the bushy stunt of the wheat can be rapidly determined on molecular scale, the identification period is short and the reliability is high, thereby having relatively great breeding application value.

Description

technical field [0001] The invention relates to a method for quickly evaluating the resistance of varieties of wheat bush dwarf disease by detecting the proliferation speed of viruses in the coleoptile with short identification period and high reliability, which belongs to the identification technology of plant disease resistance. Background technique [0002] Wheat is the general designation of Triticum plants, a gramineous plant widely planted all over the world, which originated in the Fertile Crescent region of the Middle East. Wheat is one of the three major grains, most of which are used as food, and only about one-sixth is used as feed. As the second largest food crop in the world, wheat is also the second largest food crop in China, and plays an important role in the food of Chinese people. According to statistics, wheat accounts for about 43% of the total grain consumption in China. In recent years, the world's population has grown and people's living standards ha...

Claims

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Application Information

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IPC IPC(8): C12Q1/70C12Q1/68
CPCC12Q1/18C12Q1/6851C12Q1/70C12Q1/701C12Q2531/113C12Q2545/101
Inventor 不公告发明人
Owner 江苏省金地种业科技有限公司
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