Method for rapid evaluation of variety resistance to bushy stunt of wheat by detecting proliferation rate of virus in coleoptiles
A technology for wheat bush dwarf disease and virus proliferation, which is applied in biochemical equipment and methods, and the determination/inspection of microorganisms, and can solve the problems of short identification period, inaccurate identification results, and long identification period.
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[0049] In 2015, the identification method of the invention was used to identify the resistance to bush dwarf disease of some of the collected wheat variety resources. Three wheat varieties, Fu'a 1, Abo and Luyeshu, were respectively set as the identification scales of high resistance, medium resistance and susceptibility. Among them, Fu'a 1 had a disease resistance performance of 51% in the indoor infection identification in 2013-2014. Among the wheat variety resource groups, it ranks 5th, Abo ranks 13th, and green leaf ripeness ranks 26th. The coleoptile collection time points were set as four time points on the 4th, 8th, 12th, and 16th days after the completion of inoculation.
[0050] Real-time fluorescence quantitative PCR obtains the RQ value of each variety cycle sampling time point, as shown in Table 1:
[0051]
[0052] Then draw the RBSDV proliferation index trend line: take the mean RQ value as the ordinate, and the sampling time point after feeding the poison as t...
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