The application discloses a method for regulating
virus proliferation in rice
planthopper. The method regulates the content of 3,5-bisphospho
phosphatidylinositol (PI(3,5)P2) to regulate the proliferation of rice black-streaked dwarf
virus in the body of Laodelphax striatellus and southern rice black-streaked dwarf
virus in the body of white-backed
planthopper. The PI(3,5)P2 inhibitor or dsRNA of PI(3,5)P2 synthesis
enzyme gene PIKfyve is applied by
microinjection, drop or spraying to reduce the content of PI(3,5)P2 in the body of Laodelphax striatellus and white-backed
planthopper, thereby inhibiting the proliferation of rice black-streaked dwarf virus in the body of Laodelphax striatellus and southern rice black-streaked dwarf virus in the body of white-backed planthopper. The PI(3,5)P2 homolog PI(3,5)P2-diC8 is applied to Laodelphax striatellus to increase the content of PI(3,5)P2, thereby promoting the proliferation of rice black-streaked dwarf virus in the body of Laodelphax striatellus. The application provides a new method for controlling
virus diseases transmitted by vector insects.