49 results about "Laodelphax striatella" patented technology

The present invention discloses applications of guaiacol in a rice stripe disease, wherein the applying concentration of the guaiacol liquid is 50-500 [mu]g/mL, and an emulsifying agent is added to the guaiacol liquid as a surfactant. According to the present invention, the guaiacol is used for rice stripe disease prevention and treatment, the rice stripe disease prevention and treatment effect of the guaiacol can achieve 53%, and the guaiacol is combined with the chemical pesticide for virus transmission mediator Laodelphax striatellus prevention and treatment so as to achieve the effect of concurrent disease and pest treatment; and experiment results prove that the guaiacol can be used for effectively preventing and treating the rice stripe disease.

Belonging to the field of agricultural biotechnology, the invention relates to a laodelphax striatellus lethal gene fragment. By a laboratory improved dsRNA feeding method, the invention, from laodelphax striatellus, screens out the laodelphax striatellus lethal gene fragment Ribosomal protein L9-B which can lead to laodelphax striatellus death and has sequences as shown in SEQ ID No.1. Feeding laodelphax striatellus with the dsRNA of the gene fragment provided in the invention can have good lethal effect, thus providing sequence and data bases for establishing new strategies of pest control with RNA interference technology.

The invention belongs to the field of agricultural and biological technology and relates to a Laodelphax striatellus lethal gene fragment thereof. The invention is to screen the Laodelphax striatellus lethal gene fragment Tubulin, which can lead to the death of Laodelphax striatellus after interference and has a sequence represented by SEQ ID No.1, from Laodelphax striatellus by using a laboratory improved dsRNA feeding method. When the dsRNA of the gene fragment is used for feeding Laodelphax striatellus, the maximum adjusted mortality of the Laodelphax striatellus may reach 67.4 percent, and a sequence basis and a data basis are provided for the construction of a new policy of controlling pests by using RNA interference technology.

The invention belongs to the technical field of agricultural biology, and in particular relates to a gene silencing technology-based lethal gene fragment Chitinase 7 of laodelphax striatellus and dsRNA (double-stranded RNA) thereof. In the patented invention, a dsRNA feeding method improved in laboratories is used for screening out the lethal gene fragment Chitinase 7 of the laodelphax striatellus as shown in SEQ ID NO. 1 from laodelphax striatellus, wherein the lethal gene fragment Chitinase 7 of the laodelphax striatellus can lead to the death of laodelphax striatellus after being interfered; the dsRNA of the gene fragment is used for feeding the laodelphax striatellus so as to ensure that the corrected death rate of the laodelphax striatellus reaches more than 65%; and the lethal gene fragment Chitinase 7 and the dsRNA thereof provide a sequence and data basis for establishing a new strategy of controlling injurious insects by using a RNA interference technology.

The invention relates to a drug-resistant gene CYP6AY3v2 of a small brown planthopper, a gene segment for reducing the drug resistance of the small brown planthopper and application thereof. A sequence of the drug-resistant gene CYP6AY3v2 of the small brown planthopper is shown as SEQ ID NO.1. A sequence of the gene segment for reducing the drug resistance of the small brown planthopper is shown as SEQ ID NO.3. The gene segment is used for reducing the drug resistance of the small brown planthopper. The drug-resistant gene CYP6AY3v2 and the gene segment can be used for detecting the drug resistance of the small brown planthopper and achieving the drug resistance management of the small brown planthopper.

The invention provides a method for detecting rice stripe virus in single-head laodelphax striatellus through dot-ELISA (dot-enzyme-linked immuno sorbent assay), and belongs to the field of plant protection. According to the method, after laodelphax striatellus is collected and ground, and a dot-ELISA system for detecting the rice stripe virus carried by single-head mediator laodelphax striatellus is established by monoclonal antibody of the rice stripe virus. The detection method is high in sensitivity and specificity, is suitable for large-batch single-head laodelphax striatellus sample detection, does not require special instruments and equipment, has the characteristics that the result is simple and easy to determine, the detection result is convenient to store for a long time and the like, can be used for performing large-scale survey and detection on the virus carried rate of the rice stripe virus carried by field laodelphax striatellus, and provides technical support for prediction of outbreaks of the field rice stripe disease and adoption of corresponding prevention and control measures .

The invention discloses a method for recognizing maize rough dwarf disease resistance by virus transmission inoculation of laodelphax striatellus, and belongs to the technical field of plant disease resistance recognition. Through the steps of laodelphax striatellus feeding, maize rough dwarf virus source cultivation, laodelphax striatellus feeding, laodelphax striatellus inoculation and maize rough dwarf virus resistance evaluation, the maize rough dwarf virus resistance can be efficiently, reliably and stably recognized, and a foundation is laid for mining and recognition of maize variety resources, variety resistance evaluation, disease-resistant variety breeding and resistance inheritance.

The invention discloses a method and an artificial feed for enabling laodelphax striatellus to obtain rice stripe viruses. The method for enabling rice stripe viruses to obtain laodelphax striatellus comprises the following step: feeding the laodelphax striatellus with the artificial feed containing rice stripe viruses, thereby enabling the laodelphax striatellus to obtain rice stripe viruses. The composition of the artificial feed is that every 1 muL of the artificial feed contains the rice stripe viruses greater than or equal to 300ng, the balance being a sucrose aqueous solution with concentration being 50g/L. According to the method, the rice stripe virus acquiring rate is higher than 90% which is greatly increased in comparison with that of a conventional method of feeding non-toxic ash-free plant hoppers by toxic rice plants. According to the method, an experimental platform for enabling the laodelphax striatellus to obtain toxin efficiently is established, and the research on the transmission mechanism of rice stripe viruses in the laodelphax striatellus is strongly promoted, and the effective progress of the novel disease-resistant strategy of removing virus in insects is promoted.

The granular pesticide preparation for preventing and controlling smaller brown planthopper in wheat field consists of chlorpyrifos, DDVP, stabilizer A, stabilizer B, colorizing agent and carrier in certain weight proportion. The stabilizer A is butyrolactone, gamma-propiolactone or delta-propiolactone; the stabilizer B is 1, 2-butanediol, 2-methyl-2, 4-pentanediol or [1, 5]-pentanediol; the colorizing agent is red powder, phthalocyanine red or acid scarlet; and the carrier is coal slack, perlite or vermiculite. The granular pesticide preparation as re-compounded pesticide has synergistic effect of killing smaller brown planthopper and other effect.

The invention discloses a laodelphax striatellus lethal gene fragment ATPase and application thereof. The invention screens out the lethal gene fragment ATPase of the grey planthopper which can causethe death of the grey planthopper after interference as shown in SEQ ID NO. 1, The dsRNA of the gene fragment is used for feeding the grey planthopper and the brown planthopper, which can effectivelykill the grey planthopper and the brown planthopper. The invention is safe for the environment, ecology and food, and provides a new way for establishing the RNA interference technology for controlling pests.

The invention discloses a yeast-like symbiote and use thereof. The yeast-like symbiote is obtained through being separated from a fat body of laodelphax striatellus by an in-vitro culture technology, is named Pichia anomala SP2014 through microbial taxonomic identification, has the collection number of CCTCC No: M 2014534, and is collected at the CCTCC (China Center for Type Culture Collection) at Oct. 17, 2014. Shown by research and analysis on the sensitivity of the yeast-like symbiote to different microbicides, two kinds of microbicides, namely Antracol and Nativo, with obvious inhibitory effects to P. anomala SP2014 are sprayed to wheat seedlings at the concentration of 800mg/L, the death rate reaches 46.7% and 63.3% respectively after the wheat seedlings are eaten by laodelphax striatellus, the death rate is remarkably higher than that of control, and the weight is remarkably lower than that of a control group. Proven by fluorescent quantitative detection results, the amount of yeast-like symbiotes in laodelphax striatellus bodies is remarkably reduced after the microbicides are used. The yeast-like symbiote can serve as an indication marker for monitoring vitality of laodelphax striatellus or be used for screening or producing pesticides for preventing and treating laodelphax striatellus.

The invention discloses application of protein WRKY78 to regulation of the biological stress resistance of plants. An amino acid sequence of the protein WRKY78 is as shown in sequence 2 in a sequencetable. The expression quantity and/or activity of the protein WRKY78 in oryza sativa L. spp. japonica are improved to obtain transgenic rice A; compared with the oryza sativa L. spp. japonica, the transgenic rice A is significantly improved both in rice stripe disease resistance and rice blast resistance; the expression quantity and/or activity of the protein WRKY78 in the oryza sativa L. spp. japonica are inhibited to obtain transgenic rice B; and compared with the oryza sativa L. spp. japonica, the transgenic rice B is significantly reduced in the aspects of rice stripe disease resistance, rice blast resistance and laodelphax striatellus resistance. Thus it can be seen that the protein WRKY78 can regulate the biological stress resistance of the plants. The protein WRKY78 has important application value.

The invention discloses a planthopper lethal gene segment. The planthopper lethal gene segment ubiquitin-conjugating enzyme morgue capable of leading to planthopper death after interference and with the sequence shown as SEQ ID NO.1 is screened out. When dsRNA of the gene segment is utilized and fed to small brown rice planthoppers and brown planthoppers, the death of the small brown rice planthoppers and brown planthoppers can be effectively caused. The planthopper lethal gene segment disclosed by the invention is safety to environment, ecology and food, and a novel path is provided for utilizing an RNA interference technology to control injurious insects.

The invention discloses a planthopper lethal gene fragment and an application thereof. The planthopper lethal gene fragment beta-tubulin causing death of planthopper after interference and having thesequence shown in SEQ ID NO.1 is screened out; laodelphax striatellus and brown planthopper are fed with dsRNA of the gene fragment and can be effectively killed; the gene fragment is safe to both theenvironment ecology and food and provides a new way to control pests by using the RNA interference technology.

The invention discloses an application of xanthoxylin as a rice planthopper repellent. The field use concentration of the xanthoxylin in prevention and treatment of rice planthoppers is 10-1000 ppm ofeffective components. Under a use concentration of 1000 ppm, the xanthoxylin can reach repelling rates of 81.21%, 77.50% and 65.23% to three kinds of female adult rice planthoppers, i.e., brown planthoppers, sogatella furcifera and laodelphax striatellus respectively after the xanthoxylin is sprayed for 24 hours; and the xanthoxylin has a strong repelling effect. The rice planthopper repellent compound, namely the xanthoxylin provided by the invention is an extraction component of natural plants; and a rice planthopper repellent prepared from the xanthoxylin has the advantages of good prevention and treatment effects, simple preparation, greenness, safety, effectiveness, environmental friendliness and the like.

The invention discloses a method for rapid evaluation of variety resistance to bushy stunt of wheat by detecting proliferation rate of virus in coleoptiles. The method comprises the following steps: 1) selecting identification scale; 2) performing hypoxia induction on wheat to be detected and identifying the coleoptiles of scale variety; 3) inoculating viruliferous laodelphax striatellus into the coleoptiles and then performing dark culture; 4) performing fluorescence quantitative PCR (polymerase chain reaction) to obtain relative expression (RQ value) of RBSDV (rice black streaked dwarf virus) virus S7 and an internal control gene of various wheat varieties at period time points; 5) drawing an RBSDV proliferation index trend line; and 6) evaluating the resistance level and the resistance grade of the wheat variety to be detected to the bushy stunt of the wheat according to the RBSDV proliferation index trend line formula. According to the method disclosed by the invention, the variety resistance to the bushy stunt of the wheat is rapidly evaluated by detecting the proliferation rate of the virus in the coleoptiles, the resistance level and the resistance grade of the wheat variety to the bushy stunt of the wheat can be rapidly determined on molecular scale, the identification period is short and the reliability is high, thereby having relatively great breeding application value.

The invention discloses application of GrpE protein and a coding gene thereof as molecular targets in cultivation of resistant plants. The invention provides an application of a nucleic acid moleculeor a recombinant vector for inhibiting GrpE gene expression in preparation of a transgenic plant for resisting piercing-sucking pests. The invention also provides an application of the nucleic acid molecule or recombinant vector for inhibiting GrpE gene expression in preparation of stripe disease resistant plants. The application is achieved by inhibiting piercing-sucking pests as a propagation medium. In the process of researching the interaction of the rice laodelphax striatellus-RSV, a new gene GrpE is explored from the rice laodelphax striatellus, the rice laodelphax striatellus GrpE or the homologous gene thereof can be used as a molecular target for resistance breeding of crops for resisting piercing-sucking pests, and the expression of the GrpE or the homologous gene thereof in insects is inhibited through RNAi technology; therefore, transgenic plants with resistance to piercing-sucking pests such as laodelphax striatellus are obtained.

The invention discloses a cell apoptosis gene segment for reducing the egg laying amount of the small brown rice planthopper and application thereof. The gene sequence is shown as SEQ ID NO.1 and theamino acid sequence is shown as SEQ ID NO.2. According to the invention, dsRNA of a small brown rice planthopper cell apoptosis gene segment is synthesized through a molecular biological technique; and the egg laying amount is reduced obviously by using an egg laying amount and a gene capable of serving as an effective target for biological control of the small brown rice planthopper is found. ThedsRNA sequence for synthesizing the small brown rice planthopper cell apoptosis gene segment is shown as SEQ ID NO.5, so that the gene is effectively silenced and the degradation of RNA enzyme can bewell resisted. Meanwhile, the synthesis cost is low and thus the cell apoptosis gene segment is suitable for large-scale application and popularization; the dsRNA of the cell apoptosis gene of the small brown rice planthopper has a significant reduction effect on the egg laying amount of the small brown rice planthopper by the RNAi technology; and thus a novel path is provided for controlling pests through the RNAi technology and the use of chemical pesticides is reduced.

The invention provides a method for creating a rice material capable of blocking rice stripe virus transmission of laodelphax striatellus, and belongs to the technical field of agricultural science. According to the characteristic that rice stripe virus (RSV) glycoprotein NSvc2-N-mediated virus infects mediator laodelphax striatellus midguts, NSvc2-NS segments (720bp, soluble segments encoding NSvc2-N, namely 30-268 amino acids) are selected as exogenous genes, and are integrated into rice genomes through a transgenic technology, an obtained transgenic rice plant can significantly reduce the virus obtaining rate of the laodelphax striatellus, the virus content in the laodelphax striatellus and the virus transmission rate, and proves that the NSvc2-NS protein expressed in the transgenic plant can be competitively combined with a virus infection site located in the midgut of the mediator laodelphax striatellus, so that the virus transmission of the laodelphax striatellus is blocked. The method fills up the blank of the technology of blocking the virus transmission of the laodelphax striatellus by using the plant itself, and compared with a traditional insect control and disease prevention method, the method is simple, convenient, economical, green and efficient, and can be applied to the fields of antiviral breeding work, disease prevention and control research, insect virus transmission mechanism research and the like.