19 results about "Laodelphax striatella" patented technology

The invention discloses a rice laodelphax striatellus resistance gene OsSAMS1 and application thereof. The gene provided by the invention is a DNA (Deoxyribonucleic Acid) molecule as shown in SEQ ID NO.2 and SEQ ID NO.3. The invention also provides a method for preparing the Corresponding proteins coded by the gene provided by the invention are proteins as shown in (a) and (b): (a) an amino acid sequence as shown in SEQ ID NO.1; and (b) a protein which is formed by substitution and / or deletion and / or addition of one or more amino acid residues of the amino acid sequence shown in SEQ ID NO.1, is related to rice laodelphax striatellus resistance and is derived from SEQ ID NO.1. The rice insect-resistant related protein provided by the invention affects the insect resistance of plants. Insect-sensitive plants can resist insects by improving expression of the protein coding gene, and laodelphax striatellus-resistant transgenic plants can be cultivated by silencing the gene. The gene and the encoding protein thereof can be applied to plant genetic improvement.

According to the laodelphax striatellus juvenile hormone synthetic gene LsFAMeT and the application of the laodelphax striatellus juvenile hormone synthetic gene LsFAMeT in biological prevention and control of the laodelphax striatellus, the laodelphax striatellus juvenile hormone synthetic gene LsFAMeT is subjected to targeted inhibition, so that the oviposition of female adults can be remarkably inhibited, the population of the laodelphax striatellus is controlled, and the harm of the laodelphax striatellus to food crops is reduced. The dsRNA or interference reagent is prepared by transcription of the laodelphax striatellus juvenile hormone synthetic gene LsFAMeT, and after the dsRNA or interference reagent is injected into the laodelphax striatellus, the juvenile hormone synthetic gene LsFAMeT can be effectively inhibited, female adult laodelphax striatellus can be inhibited from laying eggs, and biological prevention and control of the laodelphax striatellus can be realized. The control of laodelphax striatellus is carried out through a biological means, the method is safe and harmless to environment ecology and food, and a new strategy is provided for green control of laodelphax striatellus.

The invention discloses rice OsARF6 and OsARF17 genes and application of the rice OsARF6 and OsARF17 genes in regulation and control of resistance of laodelphax striatellus. The protein provided by the invention is a protein as shown in (a) or (b): (a) a protein consisting of a sequence 1 in a sequence table and an amino acid sequence as shown in the sequence 1; and (b) a protein which is formed by substitution and / or deletion and / or addition of one or more amino acid residues of the sequence 1 and the amino acid sequence of the sequence 2, is related to plant insect resistance and is derived from the sequence 1 and the sequence 2. The plant insect-resistant related protein provided by the invention affects the insect resistance of plants. Insect-sensitive plants can resist insects by improving the expression of the protein coding gene, so that insect-resistant transgenic plants can be cultivated. The protein and the coding gene thereof can be applied to plant genetic improvement.

The invention discloses a method for improving the efficiency of inoculating rice stripe viruses to laodelphax striatellus, which comprises the following step of: inoculating 1.5-2 leaf age rice seedlings for 2 days according to the effective inoculation intensity of 1.5 per seedling by utilizing a laodelphax striatellus group with the rice stripe virus carrying rate of more than 90%. Under the same effective inoculation intensity, the rice stripe disease incidence rate of the laodelphax striatellus inoculated by the laodelphax striatellus group with the RSV virus-carrying rate of 90% or above is high, and the rice stripe disease incidence rate of the laodelphax striatellus inoculated by the laodelphax striatellus group with the RSV virus-carrying rate of 30% is low. Under the same inoculation time, when the effective inoculation intensity of a laodelphax striatellus group with the RSV virus-carrying rate of 30% reaches 5 per seedling, the inoculation effect that the effective inoculation intensity of the laodelphax striatellus group with the RSV virus-carrying rate of more than 90% reaches 1.5 per seedling can be achieved. The invention proves that under the same inoculation intensity and inoculation time, when the laodelphax striatellus population with the RSV virus carrying rate of more than 90% is used for inoculating rice seedlings, the inoculation efficiency is obviously improved, the investigation is easy, and the working efficiency is greatly improved while the identification accuracy is ensured.

The invention belongs to the technical field of pesticides, and particularly relates to a triazole compound, a preparation method thereof, an insecticidal composition and application. The compound is shown as a general formula I, wherein X represents CR11 or N; x2 represents CR2 or N; w represents O or S; r1, R2, R3, R4, R5, R6, R7, R9, and R11 each independently represent hydrogen, halogen, alkyl, or the like; r8 represents hydrogen, an alkyl group, an alkenyl group, an alkynyl group, or the like; r10 represents hydrogen, an alkyl group, an alkenyl group, an alkynyl group, or the like; the compound has excellent prevention and treatment effects on brown planthopper, rhopalosiphum padi, laodelphax striatellus, sogatella furcifera, green peach aphid, peanut aphid and the like.

The invention discloses an LsDHX16 gene of laodelphax striatellus coding RNA helicase and application of the LsDHX16 gene in biological control, and belongs to the technical field of biology. After the expression level of the gene is knocked down in the laodelphax striatellus by using an RNA interference technology, the external genital organ of the female laodelphax striatellus is deformed and the female laodelphax striatellus dies due to ecdysis failure, so that the sex proportion of the laodelphax striatellus is influenced; meanwhile, the development of the amphoteric nymphs is stagnated. The abnormal phenomena show that the LsDHX16 gene plays an important role in sex differentiation and growth processes of laodelphax striatellus. In view of high specificity of the gene participating in regulation and control of insect sex determination, the gene can be used as a target spot for accurately preventing and controlling laodelphax striatellus, and dsRNA targeting the gene can achieve the purpose of effectively preventing and controlling laodelphax striatellus by disturbing the sex proportion of laodelphax striatellus and reducing the population number of laodelphax striatellus. In conclusion, the invention provides a theoretical basis for biological control of laodelphax striatellus.

The application discloses a method for regulating virus proliferation in rice planthopper. The method regulates the content of 3,5-bisphospho phosphatidylinositol (PI(3,5)P2) to regulate the proliferation of rice black-streaked dwarf virus in the body of Laodelphax striatellus and southern rice black-streaked dwarf virus in the body of white-backed planthopper. The PI(3,5)P2 inhibitor or dsRNA of PI(3,5)P2 synthesis enzyme gene PIKfyve is applied by microinjection, drop or spraying to reduce the content of PI(3,5)P2 in the body of Laodelphax striatellus and white-backed planthopper, thereby inhibiting the proliferation of rice black-streaked dwarf virus in the body of Laodelphax striatellus and southern rice black-streaked dwarf virus in the body of white-backed planthopper. The PI(3,5)P2 homolog PI(3,5)P2-diC8 is applied to Laodelphax striatellus to increase the content of PI(3,5)P2, thereby promoting the proliferation of rice black-streaked dwarf virus in the body of Laodelphax striatellus. The application provides a new method for controlling virus diseases transmitted by vector insects.

The application provides a Laodelphax striatellus juvenile hormone synthesis gene LsFAMeT and application thereof in biological control of the Laodelphax striatellus, and the LsFAMeT gene can be targetedly inhibited to significantly inhibit the oviposition of female adults, thereby controlling the population of the Laodelphax striatellus and reducing the damage of the Laodelphax striatellus to grain crops. The dsRNA or interference reagent is prepared by transcription of the LsFAMeT gene, and the oviposition of female adults of the Laodelphax striatellus can be effectively inhibited after the reagent is injected into the Laodelphax striatellus, so that the biological control of the Laodelphax striatellus is realized. The biological method is used for the control of the Laodelphax striatellus, and is safe and harmless to the environment and food, thereby providing a new strategy for green control of the Laodelphax striatellus.

The invention discloses a plant anti-laodelphax striatellus related protein OsERF47 as well as a coding gene and application thereof. The protein provided by the invention is a protein as shown in (a) or (b): (a) an amino acid sequence as shown in SEQ ID NO.1; and (b) a protein which is obtained by substituting and / or deleting and / or adding one or more amino acid residues of the amino acid sequence of SEQ ID NO.1, is related to plant insect resistance and is derived from SEQ ID NO.1. The plant insect-resistant related protein provided by the invention affects the insect resistance of plants. Insect-sensitive plants can resist insects by inhibiting expression of the protein coding gene, so that insect-resistant transgenic plants can be cultivated. The protein and the coding gene thereof can be applied to plant genetic improvement.

The invention discloses a rice laodelphax striatellus resistance gene OsCNGC9 and an application thereof. The gene provided by the invention is a DNA (Deoxyribonucleic Acid) molecule as shown in SEQ ID NO.2 and SEQ ID NO.3. The invention also provides a method for preparing the Corresponding proteins coded by the gene provided by the invention are proteins as shown in (a) and (b): (a) a protein composed of an amino acid sequence as shown in SEQ ID NO.1; and (b) a protein which is formed by substitution and / or deletion and / or addition of one or more amino acid residues of the amino acid sequence shown in SEQ ID NO.1, is related to rice laodelphax striatellus resistance and is derived from SEQ ID NO.1. The rice insect-resistant related protein provided by the invention affects the insect resistance of plants. Insect-sensitive plants can resist insects by improving the expression of the protein coding gene, so that laodelphax striatellus-resistant transgenic plants can be cultivated. The gene and the encoding protein thereof can be applied to plant genetic improvement.

The application discloses OsHIR1 Application of a gene in improving resistance of rice to a white-backed planthopper, and belongs to the technical field of agricultural biological gene engineering, OsHIR1 Application of a gene in improving resistance of rice to a white-backed planthopper, and the application OsHIR1 The nucleotide sequence of the gene is shown as SEQ ID NO. 1. And a method for improving resistance of rice to a white-backed planthopper by using the gene comprises the following steps: OsHIR1 S1, cloning a rice OsHIR1 gene; S2, constructing OsHIR1 OsHIR1 an overexpression vector; S3, transforming the overexpression vector obtained in S2 into rice to obtain a transgenic plant with improved resistance of rice to a white-backed planthopper. The application provides a new selection for improving the resistance of rice to a white-backed planthopper, effectively improves the resistance of rice to a white-backed planthopper, and has great application value for breeding of rice resistant to a white-backed planthopper.

This invention provides a method for creating rice materials that can block the transmission of rice stripe virus by planthoppers, belonging to the field of agricultural science and technology. Based on the characteristic that the rice stripe virus (RSV) glycoprotein NSvc2-N mediates viral infection of the planthopper midgut, NSvc2-N is selected from this glycoprotein. S (720 bp, encoding the soluble region of NSvc2-N, i.e., amino acids 30-268), was integrated into the rice genome using transgenic technology. The resulting transgenic rice plants significantly reduced the virus acquisition rate, virus content in the insects, and virus transmission rate of planthoppers, demonstrating that the NSvc2-N expressed in the transgenic plants... S The protein can competitively bind to the viral infection site located in the midgut of the planthopper, thereby blocking the planthopper from transmitting the virus. This method fills the gap in technology that uses the plant itself to block the planthopper from transmitting the virus. Compared with traditional "insect control and disease prevention" methods, this method is simple, economical, green, and efficient, and can be applied to antiviral breeding, disease control research, and research on the mechanism of insect virus transmission.

The invention discloses a rice laodelphax striatellus-resistant related protein OsPME19 as well as a coding gene and application thereof. The protein provided by the invention is a protein as shown in (a), (b) or (c): (a) an amino acid sequence as shown in SEQ ID NO.1; (b) an amino acid sequence as shown in SEQ ID NO. 2; and (c) a protein which is obtained by substitution and / or deletion and / or addition of one or more amino acid residues on the amino acid sequence of SEQ ID NO.1 or SEQ ID NO.2, is related to plant insect resistance and is derived from SEQ ID NO.1 or SEQ ID NO.2. The plant insect-resistant related protein provided by the invention affects the insect resistance of plants. The protein and the coding gene thereof can be applied to plant genetic improvement.

The invention discloses application of an OsHIR1 gene in improving resistance of rice laodelphax striatellus, belongs to the technical field of agricultural biological genetic engineering, and discloses application of the OsHIR1 gene in improving resistance of rice laodelphax striatellus. The nucleotide sequence of the OsHIR1 gene is shown as SEQ ID NO.1. The invention further discloses application of the OsHIR1 gene in improving resistance of rice laodelphax striatellus. The invention also discloses a method for improving the resistance of rice laodelphax striatellus by using the OsHIR1 gene. The method comprises the following steps: S1, cloning the rice OsHIR1 gene; s2, constructing an OsHIR1 overexpression vector; and S3, transforming the overexpression vector obtained in S2 into rice to obtain a transgenic plant with improved resistance to small brown rice planthopper. The invention provides a new choice for improving the resistance of the rice to the laodelphax striatellus, effectively improves the resistance of the rice to the laodelphax striatellus, and has an important application value for breeding of the rice for resisting the laodelphax striatellus.

The invention relates to the technical field of pesticides, in particular to a saflufenacil oxane analogue as well as a preparation method and application thereof. The saflufenacil oxane analogue is a compound as shown in a formula I, or a stereoisomer, a tautomer, an isotope derivative and a pesticide acceptable salt thereof, and a biological activity test shows that the derivative has relatively good insecticidal activity on laodelphax striatellus and can be used for preventing and treating agricultural pests, particularly homoptera pests. The saflufenacil oxane analogue can improve the insecticidal activity, has a wide insecticidal spectrum, improves the drug resistance of existing compounds, and avoids or delays the generation of resistance. # imgabs0 #

The invention discloses a plant insect-resistant related protein OsMYC2 as well as a coding gene and application of the plant insect-resistant related protein OsMYC2. The OsMYC2 protein provided by the invention is a protein as shown in (a) or (b): (a) a protein consisting of a sequence 1 in a sequence table and an amino acid sequence as shown in the sequence 1; and (b) a protein which is formed by substitution and / or deletion and / or addition of one or more amino acid residues of the sequence 1 and the amino acid sequence of the sequence 2, is related to plant insect resistance and is derived from the sequence 1 and the sequence 2. The plant insect-resistant related protein provided by the invention affects the insect resistance of plants. By improving the expression of the protein coding gene, the laodelphax striatellus susceptible rice can resist insects, so that the insect-resistant transgenic rice variety can be cultivated. The protein and the coding gene thereof can be applied to plant genetic improvement.

The application discloses an application of a Laodelphax striatellus saliva gene LsSP and dsRNA thereof in preventing and treating the spread of rice stripe virus RSV by the Laodelphax striatellus, and belongs to the field of biological control of pests.The dsRNA targeting the saliva gene LsSP of the Laodelphax striatellus is synthesized by an in vitro transcription method, and is introduced into the Laodelphax striatellus, so that the expression of the saliva gene LsSP of the Laodelphax striatellus can be effectively interfered, the ability of the Laodelphax striatellus to spread the rice stripe virus RSV can be reduced, and the virus can be prevented from damaging the rice production.The application is specifically directed to the link of the Laodelphax striatellus to spread the rice stripe virus RSV, is harmless to mammals, fish and shrimps, natural enemy insects and pollinating insects, has the advantages of quick effect, environmental friendliness and the like, and has a good application prospect.

The present invention belongs to the technical field of pesticides, and specifically relates to a triazole compound and a preparation method therefor, and an insecticidal composition and the use thereof. The compound is as represented by general formula I, where X represents CR11 or N; X2 represents CR2 or N; W represents O or S; R1, R2, R3, R4, R5, R6, R7, R9 and R11 each independently represents hydrogen, halogen, alkyl, etc.; R8 represents hydrogen, alkyl, alkenyl, alkynyl, etc.; and R10 represents hydrogen, alkyl, alkenyl, alkynyl, etc. The compound exhibits a good control effect on Nilaparvata lugens, Rhopalosiphum padi, Laodelphax striatellus, Sogatella furcifera, Myzus persicae, Aphis craccivora, etc.

The present application proposes a molting gene LsMH of gray planthopper and its application in biological control of gray planthopper. As one of the key genes for molting of gray planthopper, targeted interference with the molting gene LsMH of gray planthopper can significantly inhibit the emergence of gray planthopper into adults, causing the death of gray planthopper, thereby controlling the gray planthopper population and reducing the damage of gray planthopper to food crops. Controlling gray planthoppers by biological means is safe and harmless to the environment, ecology and food, and provides a new strategy for green control of gray planthoppers. dsRNA or interference reagents are prepared by transcribing the molting gene LsMH of gray planthoppers, and can effectively interfere with the LsMH gene after being injected into gray planthoppers. During the molting process, the nymphs of gray planthoppers have bent or incomplete wings, shriveled abdomens, and incomplete molting, which inhibits the emergence of gray planthoppers into adults, causes the death of gray planthoppers, and achieves biological control of gray planthoppers.