Method for recognizing maize rough dwarf virus resistance by virus transmission inoculation of laodelphax striatellus

A technology for corn rough dwarf and BPH, applied in biochemical equipment and methods, microbe measurement/inspection, grain cultivation, etc., can solve the problems of large identification groups, high cost of virus detection, and large site occupation, and achieve The result is highly effective

Inactive Publication Date: 2021-06-01
INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
View PDF3 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the prior art has the following disadvantages: 1. Using the maize flowering stage to investigate the incidence of rough dwarf disease, the identification period is long, the identification population needs to be large, and the site is large; 2. The proliferation of RBSDV in maize varieties is related to maize resistance , the detection of virus accumulation can determine its resistance at the molecular level at the seedling stage of maize, but the cost of virus detection is high; 3. Maize resistance to rough dwarf disease is a quantitative genetic trait, controlled by multiple genes, and screened by molecular markers Resistance germplasm materials need to detect multiple gene loci, and only one molecular marker cannot fully reflect the resistance of maize germplasm

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for recognizing maize rough dwarf virus resistance by virus transmission inoculation of laodelphax striatellus
  • Method for recognizing maize rough dwarf virus resistance by virus transmission inoculation of laodelphax striatellus
  • Method for recognizing maize rough dwarf virus resistance by virus transmission inoculation of laodelphax striatellus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Evaluation of corn rough dwarf resistance:

[0045] Corn rough dwarf disease causes shortening of plant internodes, and severe cases lead to poor powder loosening of tassels and deformed ears. Corn rough dwarf disease can show typical symptoms at the earliest 3-leaf stage and slow growth. After the onset of 17-leaf stage at the latest, only the upper leaves Show wax tear-like protrusions, tassels become shorter.

[0046]From 2015 to 2017, 3 highly susceptible inbred lines (5003, Ye 478, Ye 107), 2 susceptible inbred lines (Huangzao 4, Dan 360), 1 The resistance of the disease-resistant inbred line (Ji 35) and three high-resistant inbred lines (90110, Shen 137, DB544) (Table 1). The survey showed that the highly susceptible inbred lines had the disease at the 3-leaf stage, the most at the 4-leaf stage, all at the 6-leaf stage, and the most diseased plants were at the 4th stage, accounting for 78.97% of the total number of investigated plants; the susceptible inbred line...

Embodiment 2

[0055] experiment one:

[0056] 1. Feeding of SBPH:

[0057] On April 15, 2018, 10 wheat plants were sown in flower pots with a diameter of 10 cm. When the wheat grew to 4 cm, a glass tube with a diameter of 8 cm and a glass tube made of 60-mesh gauze was inserted into the flower pot to cover it. For wheat, healthy adults of SBPH collected in the field were inoculated on the wheat seedlings at a ratio of 1:1 male to female, and the upper opening of the glass yarn tube was covered with fine gauze and tied tightly with a rubber band, and the breeding was carried out at a room temperature of 20°C, and the wheat was regularly checked. Whether the stems, sheaths and leaves of SBPH have "spawning marks" of SBPH. After SBPH lay eggs, transfer SBPH to a pot of new wheat seedlings to continue to lay eggs and reproduce, and replace the wheat seedlings with glass yarn tubes Cover and continue the cultivation of wheat seedlings until the nymphs of SBPH hatch, and each pot can obtain 2000...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
heightaaaaaaaaaa
Login to view more

Abstract

The invention discloses a method for recognizing maize rough dwarf disease resistance by virus transmission inoculation of laodelphax striatellus, and belongs to the technical field of plant disease resistance recognition. Through the steps of laodelphax striatellus feeding, maize rough dwarf virus source cultivation, laodelphax striatellus feeding, laodelphax striatellus inoculation and maize rough dwarf virus resistance evaluation, the maize rough dwarf virus resistance can be efficiently, reliably and stably recognized, and a foundation is laid for mining and recognition of maize variety resources, variety resistance evaluation, disease-resistant variety breeding and resistance inheritance.

Description

technical field [0001] The invention belongs to the technical field of identification of plant disease resistance, and in particular relates to a method for identifying resistance to rough dwarf disease of corn by using striatellus striatellus. Background technique [0002] Corn rough dwarf disease is an outbreak and epidemic virus disease that seriously harms corn. After the onset of corn, not only the plant height is significantly reduced, but also the tassels are poorly developed, the powder is less, the ears are slightly shorter and the grains are few, and the tassels cannot be pulled out or There is no pollen, and the ears are deformed and fruitless or very few, resulting in severe yield reduction. Maize rough dwarf disease was first discovered in northern Italy and spread throughout Europe and Asia. It was first discovered in southern Xinjiang and western Gansu in my country in 1954, first occurred in Hebei and Zhejiang in the early 1960s, and spread again in my countr...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/18C12Q1/70A01K67/033A01G22/20
CPCC12Q1/18C12Q1/70A01K67/033A01G22/20
Inventor 邸垫平路银贵张爱红杨菲田兰芝苗洪芹
Owner INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap