Method for rapidly evaluating rice SRBSDV variety resistance through detecting viral multiplication speed in coleoptile

A virus proliferation and coleoptile technology, applied in the field of agricultural science, can solve the problems of long identification cycle and low accuracy

Inactive Publication Date: 2015-07-22
创世纪复交生物科技发展有限公司
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Problems solved by technology

[0012] The purpose of the present invention is to provide a short identification period and high accuracy for quickly evaluating the resistance of rice SRBSDV varieties by detecting th

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  • Method for rapidly evaluating rice SRBSDV variety resistance through detecting viral multiplication speed in coleoptile

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Embodiment

[0050] In 2015, the identification method of the invention was used to identify the SRBSDV resistance of some of the collected rice variety resources. Three rice varieties, Shunyou 109, IR24 and Fengliangyou 4, were respectively set as the identification scales of high resistance, medium resistance and susceptibility. Among them, Shunyou 109 had a disease resistance performance of 138 in the indoor infection identification in 2013-2014. Among the rice variety resource groups, it ranks 11th, IR24 ranks 28th, and Fengliangyou 4 ranks 69th. The time points for coleoptile collection were set at four time points: the 4th day, the 6th day, the 8th day, and the 10th day after the inoculation was completed, and three replicates were collected for each time point of each variety.

[0051] Real-time fluorescence quantitative PCR obtains the RQ value of each variety cycle sampling time point, as shown in Table 1:

[0052]

[0053] Then draw the SRBSDV proliferation index trend line: ta...

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Abstract

The invention discloses a method for rapidly evaluating rice SRBSDV variety resistance through detecting a viral multiplication speed in coleoptile. The method comprises the following steps: (1) selecting an identifying scaleplate; (2) performing hypoxia induction on the rice to be tested and identifying the coleoptile of the scaleplate variety; (3) inoculating the toxic sogatella furcifera on the coleoptile and performing dark culture; (4) performing fluorogenic quantitative PCR to obtain a relative transcript level (RQ value) of an SRBSDV virus S9 and a reference gene in each rice variety period time; (5) drawing an SRBSDV proliferation index trend line; and (6) evaluating the resistance level and the resistance grade of the rice variety to be tested on the Southern rice black-streaked dwarf virus according to the SRBSDV proliferation index trend line formula. By detecting the viral multiplication speed in the coleoptiles and rapidly evaluating the resistance of the rice SRBSDV germplasm resistance, the resistance level and the resistance grade of the rice variety on the SRBSDV from the molecular level in the rice germination stage are determined, the identification period is short and the reliability is high, and the method has big breeding application value.

Description

technical field [0001] The invention relates to the field of agricultural science and technology, in particular to a method for quickly evaluating the resistance of rice SRBSDV varieties by detecting the virus proliferation speed in the coleoptile. Background technique [0002] Rice is a plant of the genus Oryza in the order Cyperaceae, Poaceae, Monocotyledons, Angiosperms. Rice is one of the three most important food crops, the sown area accounts for 1 / 5 of the grain sown area, the annual output is about 480 million tons, accounting for 1 / 4 of the world's total grain output, and more than half of the world's population Rice is the staple food and one of the most important cultivated crops in my country. Rice is native to tropical Asia and is one of the world's main food crops. my country's rice sowing area accounts for 1 / 4 of the country's grain crops, while the output accounts for more than half. The cultivation history has been 6000-7000 years. It is an important food ...

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Application Information

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IPC IPC(8): C12Q1/70C12Q1/68
Inventor 不公告发明人
Owner 创世纪复交生物科技发展有限公司
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