Method for rapidly evaluating rice SRBSDV variety resistance through detecting viral multiplication speed in coleoptile
A virus proliferation and coleoptile technology, applied in the field of agricultural science, can solve the problems of long identification cycle and low accuracy
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[0050] In 2015, the identification method of the invention was used to identify the SRBSDV resistance of some of the collected rice variety resources. Three rice varieties, Shunyou 109, IR24 and Fengliangyou 4, were respectively set as the identification scales of high resistance, medium resistance and susceptibility. Among them, Shunyou 109 had a disease resistance performance of 138 in the indoor infection identification in 2013-2014. Among the rice variety resource groups, it ranks 11th, IR24 ranks 28th, and Fengliangyou 4 ranks 69th. The time points for coleoptile collection were set at four time points: the 4th day, the 6th day, the 8th day, and the 10th day after the inoculation was completed, and three replicates were collected for each time point of each variety.
[0051] Real-time fluorescence quantitative PCR obtains the RQ value of each variety cycle sampling time point, as shown in Table 1:
[0052]
[0053] Then draw the SRBSDV proliferation index trend line: ta...
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