A molecular identification method for rice black-streaked dwarf resistance
A rice black-streaked dwarf and molecular identification technology, which is applied in the molecular identification of rice black-streaked dwarf resistance and the field of molecular identification of rice black-streaked dwarf resistance, can solve the problem of short identification period and long identification period , low accuracy and other issues
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[0045] In 2015, using the identification method of the invention, the black-streaked dwarf disease resistance identification was carried out on some of the collected rice variety resources. The specific steps are as follows:
[0046] 1) Acquisition of poisonous SBPH and identification scale: Collect senior SBPH nymphs in the field, purify, screen and preserve them for passage. Before identification, use RBSDV poison source to feed 2-instar SBPH nymphs to obtain poisonous SBPH; according to In the artificial inoculation identification, three identification scales are set for the resistance performance of different varieties to rice black-streaked dwarf disease: high resistance, medium resistance, and susceptibility. The specific operation steps are as follows:
[0047] 1.1) Acquisition of RBSDV virus source: Collect diseased rice plants showing symptoms of rice black-streaked dwarf disease at the peak tillering stage from the field in the severely affected area of rice black-s...
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