52 results about "Genome comparison" patented technology

The invention relates to an NGS-based chromosome equilibrium translocation detection and analysis system and application, and belongs to the technical field of gene detection biological information. The detection and analysis system comprises a data acquisition module, a genome comparison module, a sliding cutting module, a short sequence comparison module, a breakpoint analysis module and a result output module. Firstly, reads which cannot be simultaneously compared to a human reference genome at two ends are screened out and cut into continuous short sub-sequences by sliding; the sub-sequences are compared to the human reference genome again, so that the reads sequence originally comprising balanced translocation points can be compared with the human genome through short fragments aftercutting; and through clustering grouping of comparison positions and a correlation covariance weighting algorithm of corresponding sub-sequence numbers, reads of which the two ends are respectively compared with different chromosomes can be quickly screened out, and a balanced translocation breakpoint is accurately positioned, so that a sequence designed by a primer is provided for subsequent Sanger sequencing verification. The application range comprises NIPT, PGS, PGD, common NGS sequencing results and the like.

The invention discloses a secondary approach transformation method based on an instruction of an FK506 production bacterial strain wave chain streptomycete genome scale metabolic network model. The model is based on annotation genes and physiology and biochemistry information. By comparing and analyzing the model with a streptomyces coelicolor genome, metabolic genes are found being highly conservative. Metabolic flux analysis is performed on a genome scale metabolic network, and therefore the model predicts a mutation bacterium secondary approach gene cluster transformation strategy for improving a production level. According to the secondary approach transformation method based on the instruction of the FK506 production bacterial strain wave chain streptomycete genome scale metabolic network model, the transformation method utilizes the genome scale metabolic network model to predict special structural genes in an FK506 bacterial strain secondary approach gene cluster, the production level of bacterial strains after transformation is improved by 20 percent to 90 percent, the special structural genes in the gene cluster are augmented to improve production capacity, and large application value is achieved in secondary approach rational transformation of microorganism immunosuppressor production bacterial strains. The high-efficiency and systematic method is provided for optimizing of the bacterial strains.

The invention discloses a genomic-data storage method. The genomic-data storage method includes the steps that in the genome comparison process, gene-sequence comparison information is obtained, and gene-sequence statistical information is established; the gene-sequence comparison information is stored in a magnetic disk, and according to comparison positions of the gene-sequence comparison information in a genome, corresponding indexes are stored in a memory, wherein the indexes are storage positions of the gene-sequence comparison information in the magnetic disk; genome statistical information is classified, and first statistical information and second statistical information are obtained; the first statistical information is stored in the memory, wherein the first statistical information is statistical information with the access frequency higher than the preset frequency in the variation detection process; the second statistical information is stored in the magnetic disk, wherein the second statistical information is statistical information which cannot be stored in the memory and / or statistical information with the access frequency lower than the preset frequency in the variation detection process. The invention also discloses an electronic device with the genomic-data storage method.