Molecular marker primer composition for identifying inter-hybrid seeds of pears and apples and application thereof

Abstract

The invention discloses a molecular marker primer composition for identifying inter-hybrid seeds of pears and apples and an application thereof. Specific sequences of pear and apple genomes are detected by a method of whole genome comparison, seven pairs of molecular marker specific primers P13, P31, P33, A31, A33, A34 and A37 are developed newly, and positive and negative primer sequences are asshown in a table 1. By identifying the inter-hybrid offsprings of pears and apples by means of the specific primers, specific amplified strips of the pears, apples and hybrid offsprings can be obtained, separately, that is, the inter-hybrid seeds of the pears and the apples are detected truly. The molecular marker based on the specific sequences of the genomes can be used for identifying the inter-hybrid seeds of pears and apples and has important theoretical and practical guiding meaning in improving genetic breeding progress of fruit trees and accelerating molecular assisted selective breeding.

Description

technical field [0001] The invention belongs to the field of molecular genetic breeding, and provides a molecular marker primer composition for identifying hybrids between pear and apple genera and applications thereof. Background technique [0002] Apple (Malus Domestica Borkh) and pear (Pyrus L.) belong to the Rosaceae family and are important fruit tree species cultivated worldwide. At the same time, my country is the main origin of pears and apples, with a very long history of cultivation and rich genetic diversity , there are many local varieties with good quality, which are still the main planted varieties at present. However, along with the continuous improvement of economic development and people's living standards, the market needs more abundant and high-quality varieties of pears and apples to meet consumer demand. [0003] Distant hybridization, as a breeding method that breaks through the gene pool of the population and obtains excellent genetic material from oth...

AI Technical Summary

Problems solved by technology

Generally based on the method of morphological observation, identification is usually mainly based on the morphological characteristics of leaves, flowers, and fruits. Although the method is simple and intuitive, the identification process is subject to the subjective influence of the observer; at the same time, the observation index is often affected by the external environment. The influence of factors will also interfere with the identification results; while fruit trees usually have a childhood period of 5-8 years, it takes a long time to observe the fruit shape, and it is difficult to be identified in a short time

Using the chromosome identification method, species with different chromosome numbers can be easily identified. However, pears and apples belong to the Rosaceae family and have the same number of chromosomes (2n=34), so it is difficult to distinguish

The method of isoenzyme marking has been used to identify distant hybrid varieties, but its development is restricted by the lack of polymorphic sites and cumbersome and time-consuming operations.

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