41 results about "Metabolic network model" patented technology

The invention discloses a saccharopolyspora spinosa genome scale metabolic network model and a construction method and application of the saccharopolyspora spinosa genome scale metabolic network model. The construction method includes the following steps that according to annotation information of saccharopolyspora spinosa genome sequences in KEGG and NCBI databases, a characteristic reaction for spinosad biosynthesis and a thallus sythesis reaction are added, a network reaction is manually refined, and then the saccharopolyspora spinosa genome scale metabolic network model is acquired. Through the saccharopolyspora spinosa genome scale metabolic network model, influences on improvement of the output of spinosad on a gene target spot can be predicted, the modification direction is finally determined, and the bacterial strain path molecule modification method is achieved. Experiments show that through genes predicted by the saccharopolyspora spinosa genome scale metabolic network model, genetically engineered bacteria obtained through modification can make the output of spinosad improved by 86.5% compared with wild bacterial strains. An instruction platform is provided for construction, research and analysis of the bacterial strains for efficiently producing and synthesizing spinosad.

The invention discloses a secondary approach transformation method based on an instruction of an FK506 production bacterial strain wave chain streptomycete genome scale metabolic network model. The model is based on annotation genes and physiology and biochemistry information. By comparing and analyzing the model with a streptomyces coelicolor genome, metabolic genes are found being highly conservative. Metabolic flux analysis is performed on a genome scale metabolic network, and therefore the model predicts a mutation bacterium secondary approach gene cluster transformation strategy for improving a production level. According to the secondary approach transformation method based on the instruction of the FK506 production bacterial strain wave chain streptomycete genome scale metabolic network model, the transformation method utilizes the genome scale metabolic network model to predict special structural genes in an FK506 bacterial strain secondary approach gene cluster, the production level of bacterial strains after transformation is improved by 20 percent to 90 percent, the special structural genes in the gene cluster are augmented to improve production capacity, and large application value is achieved in secondary approach rational transformation of microorganism immunosuppressor production bacterial strains. The high-efficiency and systematic method is provided for optimizing of the bacterial strains.

The invention discloses a method for co-producing chiral hydroxyl esters of uridine phosphoryl compounds. The method uses orotic acid and β-keto ester compounds as substrates, uses glucose as an energy donor, and utilizes permeable Simultaneous production of uridine phosphoryl compounds and chiral hydroxyl esters by yeast cells. The present invention utilizes the principles of whole-cell catalysis and metabolic engineering, establishes a metabolic network model and analyzes metabolic flow, and utilizes permeable yeast cells to co-produce uridine phosphoryl compounds and chiral hydroxyl esters, increasing the yield and reducing production costs , The synthesis time is shortened, and the utilization rate of the substrate is greatly improved.

The invention discloses a preparation method of citicoline, which uses choline chloride, phosphate ion and cytidine monophosphate or its precursor as substrates, and uses glucose, fructose, sucrose or maltose as energy donors. Add small molecular chemical effect substances, and use permeable yeast cells to catalyze the preparation of citicoline. The present invention establishes a metabolic network model and analyzes metabolic fluxes, adopts small molecular chemical effect substances to regulate metabolic fluxes, thereby improving energy self-coupling and choline phosphorylation efficiency, and utilizes permeable yeast cells to efficiently prepare citicholine Alkali, the concentration of the product is greatly increased, and the utilization rate of the substrate is also improved.