118 results about "Pullularia pullulans" patented technology

Three choline utilizing strains of microorganisms isolated from the anthers of wheat, Aureobasidium pullulans strain AS 55.2, Arthrobacter species strain OH 221.3, and Pseudomonas species strain AS 64.4, are superior antagonists of F. graminearum. These microorganisms are effective for suppression and control of FHB in cereals, particularly in wheat and barley.

The invention provides mutant aureobasidium pullulans TKPM00006 for massively producing beta-poly(malic acid) and a method for producing poly(malic acid) by using the mutant through fermentation. The strain is the mutant for producing beta-poly(malic acid) with high yield, which is cultured by multiple groups of composite mutagenesis technology based on beta-poly(malic acid) producing TKPM10017 screened in the soil of Wang Taibao orchard, Yinchuan, Ningxia Hui Autonomous Region, China. Under the optimization condition, the yield of beta-poly(malic acid) reaches 18.4+/-1.3g/l.

The invention relates to a fire-retardant polyethylene cable material with mould-proof function, which is characterized by comprising the following components by weight portion: 45 to 70 portions of high density polyethylene resin, 30 to 55 portions of linear low density polyethylene resin, 2.35 to 2.85 portions of carbon black, 35 to 65 portions of fire retardant, 3 to 6 portions of lubricant, 0.5 to 2 portions of antioxidant, and 1 to 2 portions of antibacterial agent. The method for preparing the fire-retardant polyethylene cable material with the mould-proof function comprises the following steps: weighing raw materials according to a formulation, mixing the materials, and performing the processes of extruding, granulating and drying the materials to prepare the mould-proof fire-retardant polyethylene cable material. The mould-proof fire-retardant polyethylene cable material can effectively inhibit the growth, the forming and the reproduction of eight types of mould fungi including aspergillus niger, aspergillus terreus, aureobasidium pullulans which can easily grow on a cable under the condition that the original performances and the cabling process of the fire-retardant polyethylene cable material are not influenced, thus the cable is prevented from being infringed by the mould fungi, and a number of unnecessary loss and hidden hazards to the society are avoided.

The present invention relates to utilizing saccharide metabolism to produce extracellular water soluble polysaccharide and belongs to the field of bioengineering technology. The present invention provides strain, named Aureobasidium pullulans G-58 and with the preservation number of CGMCC No. 1234. The pullulan producing process with the strain includes fermentation culture, fermented liquid post-treatment, heat treatment, decolorizing, precipitation, desalting, drying and other steps. The pullulan product has light color, high polysaccharide content, excellent viscoelasticity, excellent emulsifying performance and plasticity, and no toxicity and harm, and may be used widely in food, medicine, cosmetics, etc.

The invention provides a novel method for producing beta-polymalic acid by the solid-state fermentation of microorganisms, and relates to a novel process of producing the beta-polymalic acid by using an inert adsorption composite material to the solid-state fermentation of aureobacidium pullulans(at present, the strain is stored in the general microorganism center of China Committee for Culture Collection of Microorganisms, and the CGMCC No. is 3337.). The method comprises the following steps of: selecting a nontoxic inert support which is good in physical water absorption and stable in nature and has a porous structure, evenly mixing the inert support and a liquid culture medium inoculated with the aureobacidium pullulans in a certain ratio, sending the mixture to a solid-state reactor for fermentation, extracting a substrate after the solid-state fermentation with water, and inspecting the content of the polymalic acid. By the method, the fermentation efficiency of the polymalic acid is improved, and the transfer efficiency of oxygen in the fermentation process is enhanced; at the same time, because the inert support can be recycled, the discharge of the solid wastes is greatly reduced; the utilization ratio of the fermentation equipment is high, the fermentation period is short, the products are easy to be separated and extracted, and the yield and purity of the polymalic acid are improved; and the operating cost is low, the economic benefits are obvious, and the industrialized production is facilitated.

The invention discloses a bacterial strain for producing PMLA [Poly (Beta-L-malic acid)], which is obtained by screening the surface of a plant leaf and can not produce melanin. The classification name is Aureobasidium pullulans, the Latin literature name is Aureobasisdium pullulans ZD-3D, and the preservation number of the bacterial strain is CGMCC No.4605. The method for producing PMLA by the bacterial strain is simple to implement and is beneficial to the separation and purification of PMLA, and the yield can be up to 50-60g/L.

The invention discloses a method for producing poly(malic acid) and pullulan together by using Aureobasidium pullulans. The invention relates to a method for producing poly(malic acid) and pullulan by using Aureobasidium pullulans, belonging to the technical field of the bio-fermentation engineering. The method comprises the following steps: reducing the temperature of the culture medium of a fermentation tank to 28 DEG C, inoculating a strain in the fermentation tank according to the inoculation quantity which is 3%, stirring under the conditions that the rotational speed is 200rpm and the ventilation rate is 0.5VVM, controlling the temperature to 28-31 DEG C to begin fermentation, performing the fed-batch of sucrose after 24 hours to control the content of sucrose in the fermentation liquor to 35g/L, controlling the temperature to about 25 DEG C after 72 hours, continuously performing the fed-batch of sucrose to control the content of sucrose in the fermentation liquor to 15 g/L, fermenting for 136 hours to stop adding sucrose, continuously fermenting for 8 hours when the content of sucrose in the fermentation liquor is reduced to 0, controlling the pH value to 5-7.2 in the fermentation process, and fermenting for 166 hours. By adopting the method disclosed by the invention, the raw material utilization rate can be increased, energy can be saved and the cost can be reduced.

The invention discloses aureobasidium pullulans dicarboxylic acid transport protein and a recombinant vector and application thereof. Amino acid sequence of the aureobasidium pullulans dicarboxylic acid transport protein is shown as SEQ ID NO.7, SEQ ID NO.8, SEQ ID NO.9 or SEQ ID NO.10 while nucleotide sequence of the same is shown as SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3, SEQ ID NO.4 and SEQ ID NO.5. Overexpression of dicarboxylic acid transport protein in aureobasidium pullulans can increase yield of polymalic acid, and no free malic acid is detected in fermentation liquor, so that dicarboxylic acid transport protein gene is related to synthetic transport of the polymalic acid, and a foundation is laid for increasing yield of the polymalic acid.

The invention relates to the field of microorganisms and discloses aureobasidium pullulans.The preservation number of the aureobasidium pullulans is CGMCC No.11937.The aureobasidium pullulans with the preservation number being CGMCC No.11937 uses wild aureobasidium pullulans with the preservation number being CGMCC No.3.4580 as an original strain, chemical induced mutation is performed, the aureobasidium pullulans is obtained through screening, the capacity that the aureobasidium pullulans ferments to generate pullulans is remarkably improved, compared with the original strain, the yield of the pullulans is increased by 80%, and the glucose conversion rate is increased by 68%.The color of the finished pullulans becomes lighter obviously.Thus, the aureobasidium pullulans with the preservation number being CGMCC No.11937 can be widely applied to pullulans fermentation.

The invention discloses a nitrogen, phosphorus and potassium microbial coated fertilizer and a preparation method thereof. The nitrogen, phosphorus and potassium microbial coated fertilizer comprises a pill core, a middle layer and a mycoderm layer from inside to outside in sequence; the pill core comprises the following raw materials: urea, monopotassium phosphate, diammonium phosphate, calcium superphosphate, potassium sulphate, plant ash and glutinous rice glue; the middle layer comprises the following raw materials: furfural residues, vinasse, sorghum residues, edible fungus culture medium wastes, aureobasidium pullulans, EM (effective microorganism) bacterial liquid and clay; the mycoderm layer comprises the following raw materials: peat soil, a microbial agent, Carbomer, 10 wt% sodium hydroxide aqueous solution and water. The nitrogen, phosphorus and potassium microbial coated fertilizer disclosed by the invention has a viable count of more than 0.062 billion CFU/g, an organic content of more than 60.5 wt%, a pH value of 7.1-7.2 and an N, P and K content ratio of (2-3.5):(1.5-2.1):1; the reasonable collocation is performed according to nitrogen, phosphorus, potassium and other demands of crops, so that the release period of fertilizer nutrients can be prolonged; the microbial agent improves the soil texture and the tillage performance and inhibits the emergence of weeds and pests. The preparation method of the fertilizer has the advantages that the raw materials are easy to obtain, the operation is simple, the biological survival rate is high, the activity is good, and the use effect is outstanding.

The invention relates to an Aureobasidium pullulans strain and an application thereof, and belongs to the technical fields of fermentation engineering and biology. The preservation name of the Aureobasidium pullulans strain is Aureobasidium pullulans ZK-502, the strain is preserved in CGMCC (China General Microbiological Culture Collection Center) on August 20, 2018 at No.3 of No.1 West Beichen Road, Chaoyang District, Beijing, and the preservation number is CGMCC No. 16326. The Aureobasidium pullulans strain can efficiently produce liamocin by fermentation and produces no melanin, fermentation broth is light pink to faint yellow, and follow-up treatment process can be simplified. The method is simple and feasible, raw materials are simple in composition, yield of liamocin can reach 8-10 g/L, and commercial and large-scale production of liamocin is expected to be realized; the obtained liamocin product is dissoluble in water and has wide application prospect in medicine, cosmetics andfood.

The invention discloses a method for increasing the yield of pullulan. The method comprises the following steps: after activating aureobasidium pullulans, inoculating a seed medium; after OD600 is greater than 0.3, inoculating seeds into a fermentation medium; monitoring OD600 of a fermentation liquid in real time; when OD600 is greater than 0.2 and less than 0.3, adding a compounding agent I which accounts for 0.02-0.03% of the volume of the fermentation liquid, so as to greatly increase the bacterium growth velocity and remarkably shorten the logarithmic growth phase; when OD600 is greater than or equal to 0.6, adding a compounding agent II which accounts for 0.03-0.05% of the volume of the fermentation liquid. The substrate conversion speed can be accelerated, the polysaccharide synthesis performance can be improved, and the yield can be greatly increased.

The invention discloses a method for increasing the yield of aureobasidium pullulans synthesized polymalic acid. The method comprises the following steps: culturing seeds, and performing fermental cultivation, namely, adding an active agent consisting of CTTE and DMSO in the fermentation process in different stages, controlling the temperature of the fermentation process by using a stage temperature control method, setting the cultivation temperature to 25-35 DEG C for 0-40 hours, adjusting the temperature to 20-30 DEG C after 40 hours, flowing 0.1-0.14% of CTTE at a logarithmic growth stage in the fermentation process, and flowing 0.06-0.1% of DMSO at the middle fermentation stage. By the adoption of the method, the yield of polymalic acid is increased by improving permeability of cell membranes, the fermentation period is also shortened, the yield of polymalic acid is generally increased by 40%, the production cost is reduced, and the production efficiency is improved.