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118 results about "Pullularia pullulans" patented technology
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Definition of Pullularia. : a genus of fungi (family Pseudosaccharomycetaceae) forming yeastlike colonies that are at first dirty white, then streaked with dark green or black, and eventually wholly black and more or less leathery and including a form (P. pullulans) that causes discoloration of pulp and paper.
Choline-utilizing microbial strains for biologically controlling fusarium head blight
InactiveUS7601346B1Effective suppressionEffective controlBiocideBacteriaTriticeaeFusarium ear blight
Three choline utilizing strains of microorganisms isolated from the anthers of wheat, Aureobasidium pullulans strain AS 55.2, Arthrobacter species strain OH 221.3, and Pseudomonas species strain AS 64.4, are superior antagonists of F. graminearum. These microorganisms are effective for suppression and control of FHB in cereals, particularly in wheat and barley.
Owner:AGRI THE UNITED STATES OF AMERICAS AS REPRESENTED BY THE SEC OF +1
Mutant aureobasidium pullulans TKPM00006 for massively producing beta-poly(malic acid) and culture method thereof
ActiveCN101979499AHigh yieldSimplify the screening processFungiMutant preparationPullulanAureobasidium
The invention provides mutant aureobasidium pullulans TKPM00006 for massively producing beta-poly(malic acid) and a method for producing poly(malic acid) by using the mutant through fermentation. The strain is the mutant for producing beta-poly(malic acid) with high yield, which is cultured by multiple groups of composite mutagenesis technology based on beta-poly(malic acid) producing TKPM10017 screened in the soil of Wang Taibao orchard, Yinchuan, Ningxia Hui Autonomous Region, China. Under the optimization condition, the yield of beta-poly(malic acid) reaches 18.4+ / -1.3g / l.
Owner:天津慧智百川生物工程有限公司
Method for co-production of oligose and dietary fiber through wheat bran fermentation
InactiveCN102605022AEliminate separation and purificationEliminate bad effectsClimate change adaptationMicroorganism based processesPullulanAdditive ingredient
The invention relates to application of a microbial fermentation method in co-production of wheat bran oligose and dietary fiber. The invention aims to developed and utilize the wheat bran comprehensively; an efficient biological process route is adopted to deeply processing wheat bran, and the microbial fermentation method is used to prepare wheat bran dietary fiber and functional oligose simultaneously. The microbe used in the method refers to a single strain, pullularia pullulans or mixed strains of aureobasidium pulluans and agrocybe aegerita; in fermentation of wheat bran, substances in the wheat bran, such as starch and protein, can be taken as nutritional ingredients for the growth of microbes, when digesting the substances, the microbes can generate xylanase so as to hydrolyze wheat bran hemicellulose to generate oligose and prepare dietary fiber simultaneously. According to the method, the research and development idea of developing a single product is broken through, the rate of multipurpose utilization of the resource is high, the production costs is low, chemical substance residual does not exist, the environment is not polluted, a new processing technology model of 'ecological, low-carbon and efficient circulating' is formed, and the additional value of the wheat bran product is increased.
Owner:YANCHENG INST OF TECH
Fire-retardant polyethylene cable material with mould-proof function and preparation method thereof
InactiveCN101570610AGood anti-mold functionImprove performancePlastic/resin/waxes insulatorsInsulated cablesLinear low-density polyethylenePullulan
Owner:SHANGHAI KAIBO SPECIAL CABLE FACTORY
Prussian blue producing strain and prussian blue producing process with the strain
InactiveCN1609188ALight colorImprove polysaccharide yieldFungiFermentationPullulanWater soluble polysaccharides
The present invention relates to utilizing saccharide metabolism to produce extracellular water soluble polysaccharide and belongs to the field of bioengineering technology. The present invention provides strain, named Aureobasidium pullulans G-58 and with the preservation number of CGMCC No. 1234. The pullulan producing process with the strain includes fermentation culture, fermented liquid post-treatment, heat treatment, decolorizing, precipitation, desalting, drying and other steps. The pullulan product has light color, high polysaccharide content, excellent viscoelasticity, excellent emulsifying performance and plasticity, and no toxicity and harm, and may be used widely in food, medicine, cosmetics, etc.
Owner:JIANGNAN UNIV
Method for producing beta-polymalic acid by inert support adsorption solid-state fermentation
InactiveCN101696430AReduce dosageHigh yieldMicroorganism based processesFermentationMicroorganismPullulan
The invention provides a novel method for producing beta-polymalic acid by the solid-state fermentation of microorganisms, and relates to a novel process of producing the beta-polymalic acid by using an inert adsorption composite material to the solid-state fermentation of aureobacidium pullulans(at present, the strain is stored in the general microorganism center of China Committee for Culture Collection of Microorganisms, and the CGMCC No. is 3337.). The method comprises the following steps of: selecting a nontoxic inert support which is good in physical water absorption and stable in nature and has a porous structure, evenly mixing the inert support and a liquid culture medium inoculated with the aureobacidium pullulans in a certain ratio, sending the mixture to a solid-state reactor for fermentation, extracting a substrate after the solid-state fermentation with water, and inspecting the content of the polymalic acid. By the method, the fermentation efficiency of the polymalic acid is improved, and the transfer efficiency of oxygen in the fermentation process is enhanced; at the same time, because the inert support can be recycled, the discharge of the solid wastes is greatly reduced; the utilization ratio of the fermentation equipment is high, the fermentation period is short, the products are easy to be separated and extracted, and the yield and purity of the polymalic acid are improved; and the operating cost is low, the economic benefits are obvious, and the industrialized production is facilitated.
Owner:天津北洋百川生物技术有限公司
Bacterial strain for producing PMLA [Poly (Beta-L-malic acid)] and method for producing PMLA by fermentation of bacterial strain
InactiveCN102220248AEfficient fermentationThe post-processing process is simpleFungiMicroorganism based processesPullulanMicrobiology
The invention discloses a bacterial strain for producing PMLA [Poly (Beta-L-malic acid)], which is obtained by screening the surface of a plant leaf and can not produce melanin. The classification name is Aureobasidium pullulans, the Latin literature name is Aureobasisdium pullulans ZD-3D, and the preservation number of the bacterial strain is CGMCC No.4605. The method for producing PMLA by the bacterial strain is simple to implement and is beneficial to the separation and purification of PMLA, and the yield can be up to 50-60g / L.
![Bacterial strain for producing PMLA [Poly (Beta-L-malic acid)] and method for producing PMLA by fermentation of bacterial strain](https://images-eureka.patsnap.com/patent_img/bd7d7ade-f1e4-41b3-8906-fdfc7168172e/110520142403.PNG "Bacterial strain for producing PMLA [Poly (Beta-L-malic acid)] and method for producing PMLA by fermentation of bacterial strain")
Owner:SHANDONG JINCHENG BIO PHARMA CO LTD
Aureobasidium pullulans and application thereof
InactiveCN102994395AAchieve synthesisMeeting the demand for high molecular weight pullulanFungiMicroorganism based processesBiotechnologyPullulan
The invention discloses Aureobasidium pullulans and application thereof. The Aureobasidium pullulans strain SZU1001 is preserved in China center for type culture collection (CCTCC), and the preservation number is CCTCC M 2012259. A method for producing pullulan by the Aureobasidium pullulans strain includes the steps of by using the Aureobasidium pullulans strain as an original strain, performing fermentation cultivation for at least 48 hours after slope seed activation and seed culture, and extracting with an organic solvent to obtain the pullulan. The method is characterized in that the fermentation medium per liter contains glucose 40-60g, yeast powder 0.8-3.5g, ammonium sulfate 0.2-1.0g, dipotassium phosphate 1.0-3.0g, magnesium sulfate 0.1-0.3g, and sodium chloride 0-1.0g, and the pH value is 6.5-6.8. According to the invention, the aureobasidium pullulans mutant strain which can synthesize glutathione in cells can be obtained, so that the synthesis of pullulan with high molecular weight and no pigment production is realized, thereby meeting the requirement of application fields, such as medicine and environment, on the pullulan.
Owner:SUZHOU UNIV
Method for preparing beta-polymalic acid and simultaneously coproducing byproduct prussian blue
InactiveCN101100687AReduce manufacturing costHigh purityMicroorganism based processesFermentationUltrafiltrationEthanol precipitation
Beta-polyoxysuccinic acid and Prussian blue as by-product are prepared by: inoculating activated fungus in fermenting medium for 8-14 days at 24-30 deg.C and 120rpm, separating solid from liquid to remove mycelia, adding alcohol with equal volume to separate Prussian blue deposit and melanochrome, concentrating supernatant and centrifuging with double-volume alcohol to separate Prussian blue, re-concentrating, adding four volume alcohol to deposit beta-polyoxysuccinic acid, and dissolving into water, removing residual melanochrome and micro-molecular materials, and frozen drying to obtain beta-polyoxysuccinic acid, dissolving separated Prussian blue in mixed alcohol-butanone to remove melanochrome, and dialyzing and frozen drying to obtain Prussian blue. It can remove melanochrome effectively to quality purity and reduce pollution by discharged Prussian blue and melanochrome.
Owner:NANKAI UNIV
Mutant strain for mass production of pulullan and cultural method thereof
ActiveCN103060204AIncrease production capacityIncrease profitFungiMicroorganism based processesBiotechnologyMicroorganism
The invention discloses an aureobasidium pullulan for producing pullulan, and belongs to the field of microorganisms. The preservation number of the aureobasidium pullulan BCSWGHPL101 (Aureobsidium pullulans) is CGMCCNO.7055; the fermentation medium adopted by the aureobasidium pullulan for producing the pullulan comprises peptone of 3g / L, cane sugar of 100g / L, K2HPO4.7H2O of 3.0g / L, MgSO4.7H2O of 0.4g / L, NaCl of 2.5g / L and FeSO of 40.01g / L, and the pH is adjusted to 7.0. Compared with an ordinary pullulan producing strain, the mutant strain BCSWGHPL101 is higher in pullulan production capacity, and as a result, the yield of the pullulan is effectively increased, and the production cost is lowered.
Owner:天津北洋百川生物技术有限公司
Method for producing poly(malic acid) and pullulan together by using Aureobasidium pullulans
ActiveCN102492740AIncrease profitSave energyMicroorganism based processesFermentationSucrosePullulan
The invention discloses a method for producing poly(malic acid) and pullulan together by using Aureobasidium pullulans. The invention relates to a method for producing poly(malic acid) and pullulan by using Aureobasidium pullulans, belonging to the technical field of the bio-fermentation engineering. The method comprises the following steps: reducing the temperature of the culture medium of a fermentation tank to 28 DEG C, inoculating a strain in the fermentation tank according to the inoculation quantity which is 3%, stirring under the conditions that the rotational speed is 200rpm and the ventilation rate is 0.5VVM, controlling the temperature to 28-31 DEG C to begin fermentation, performing the fed-batch of sucrose after 24 hours to control the content of sucrose in the fermentation liquor to 35g / L, controlling the temperature to about 25 DEG C after 72 hours, continuously performing the fed-batch of sucrose to control the content of sucrose in the fermentation liquor to 15 g / L, fermenting for 136 hours to stop adding sucrose, continuously fermenting for 8 hours when the content of sucrose in the fermentation liquor is reduced to 0, controlling the pH value to 5-7.2 in the fermentation process, and fermenting for 166 hours. By adopting the method disclosed by the invention, the raw material utilization rate can be increased, energy can be saved and the cost can be reduced.
Owner:天津北洋百川生物技术有限公司
Process for producing pullulan
The pullulan producing process includes the following steps: 1) selectively breeding strain through ultraviolet and NTG inducing ureobasidium pullulans and screening out induced strain with less melanin secretion and high conversion rate; 2) compounding seed culture medium and fermentation culture medium; 3) culturing the obtained strain at 30-33 deg.c and inoculating in sterilized and cooled seed culture medium for culture to obtain seed liquid; 4) fermentation through setting sterilized and cooled fermentation culture medium and the obtained seed liquid in the inoculated amount of 1.5-5 % into fermentation tank to obtain fermented liquid; and 5) extracting pullulan product from the fermented liquid. The present invention has polysaccharide converting rate up to 60-70 g / L, pullulan polysaccharide yield up to 60-70 % and white or yellowish pullulan obtained.
Owner:广东富味健康科技有限公司
Method for improving poly-malic acid yield
ActiveCN103789363AIncrease productionRealize the flowMicroorganism based processesFermentationPullulanCarbon metabolism
The invention discloses a method for improving the poly-malic acid yield in aureobasidium pullulans through surface active agent tween or span. The method particularly comprises the steps that after the aureobasidium pullulans is activated, the aureobasidium pullulans is fermented under the condition containing 0.1 g / L to 0.5 g / L tween or span. The method is simple, the tween or the span can be directly added into a fermentation medium, the carbon metabolism flow direction of synthesis of the poly-malic acid and Pulullan can be adjusted, the poly-malic acid yield can be improved to a large extent, and the method has the advantages of being low in fermentation cost, high in yield, strong in technical economical efficiency, capable of being applied to poly-malic acid industrial production and the like.
Owner:SOUTHWEST UNIV
Salicornia paper type vegetable and preparation method
InactiveCN103584005AImprove food safetyFull of nutritionFood ingredient as thickening agentFood thermal treatmentNutrients proteinPullulan
The invention discloses salicornia vegetable paper and a production method of the salicornia vegetable paper. The method is characterized in that aureobasidium pullulans fermented salicornia is utilized to produce the salicornia vegetable paper. The method comprises the following basic processes: selecting fresh salicornia; cleaning; retaining color; pulping; homogenizing; seasoning and sterilizing; inoculating aureobasidium pullulans to ferment; paying on a plate to mold; baking; removing a film; segmenting; and packing. The preparation method of the vegetable paper has the advantages that an outsourcing thickening agent is not needed, and polysaccharide produced by aureobasidium pullulans fermentation is used as an endogenous thickening agent, thus the content of nutrient protein of the vegetable paper is increased, the preparation cost is decreased, and the safety in taking is improved; and the vegetable paper remains the taste of fresh salicornia, and is convenient to take, the problem that fresh salicornia is difficult to store is solved, the variety of snack foods is enriched, and the added value of the use of salicornia is also increased.
Owner:射阳县射阳港渔工贸开发有限公司
Liquid plastic mulch and preparation method thereof
ActiveCN104403135AEasy to operateGood effectClimate change adaptationMicroorganism based processesBacillus licheniformisPullulan
The invention discloses a liquid plastic film, and belongs to the technical field of agriculture. The liquid plastic film consists of the following components in percentage by weight: 1%-10% of pullulan polysaccharide, 1%-10% of polyglutamic acid and the balance of water, wherein the pullulan polysaccharide is prepared by fermenting aureobasidium pullulans of which the preservation No. is CGMCC No.7055, and the polyglutamic acid is prepared by fermenting bacillus licheniformis. The invention further discloses a preparation method and a use method of the liquid plastic film. The liquid plastic film is biodegradable and does not pollute environment, and after the plastic film is sprayed onto the surface of soil, the temperature of the soil can be raised, the evaporation of water is restrained, water content of the soil is increased, the salt content of the soil is reduced, and the soil can also be improved, so that the physicochemical property of the soil is better, and the growth of plants is promoted; besides, the liquid plastic film has the advantages of simple preparation method, low cost, good convenience in use, saving of labors and material, low cost and easiness in obtaining of raw materials, simple production equipment and technology, zero pollution in a production process, and the like.
Owner:天津北洋百川生物技术有限公司
Aureobasidium pullulans dicarboxylic acid transport protein and recombinant vector and application thereof
The invention discloses aureobasidium pullulans dicarboxylic acid transport protein and a recombinant vector and application thereof. Amino acid sequence of the aureobasidium pullulans dicarboxylic acid transport protein is shown as SEQ ID NO.7, SEQ ID NO.8, SEQ ID NO.9 or SEQ ID NO.10 while nucleotide sequence of the same is shown as SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3, SEQ ID NO.4 and SEQ ID NO.5. Overexpression of dicarboxylic acid transport protein in aureobasidium pullulans can increase yield of polymalic acid, and no free malic acid is detected in fermentation liquor, so that dicarboxylic acid transport protein gene is related to synthetic transport of the polymalic acid, and a foundation is laid for increasing yield of the polymalic acid.
Owner:SOUTHWEST UNIVERSITY +1
Aureobasidium pullulans genome scale metabolism network model and application thereof
The invention discloses an aureobasidium pullulans genome scale metabolism network model and application thereof. Construction of the aureobasidium pullulans genome scale metabolism network model comprises four steps of building of a network database, refining and calibrating of the database, building of a mathematical model and validation and analysis of the model. According to the constructed aureobasidium pullulans genome scale metabolism network model, a gene target for potentially improving polymalic acid can be predicted, a guiding platform is provided for research and comprehensive analysis of aureobasidium pullulans, and the aureobasidium pullulans genome scale metabolism network model has great significance on transformation of the aureobasidium pullulans to obtain high-yield metabolic products of the polymalic acid and pullulan.
Owner:SOUTHWEST UNIVERSITY +1
Strain producing pullulan, application thereof and pullulan production method
InactiveCN105695347AQuality improvementIncrease productionFungiMicroorganism based processesBiotechnologyMicroorganism
The invention relates to the field of microorganisms and discloses aureobasidium pullulans.The preservation number of the aureobasidium pullulans is CGMCC No.11937.The aureobasidium pullulans with the preservation number being CGMCC No.11937 uses wild aureobasidium pullulans with the preservation number being CGMCC No.3.4580 as an original strain, chemical induced mutation is performed, the aureobasidium pullulans is obtained through screening, the capacity that the aureobasidium pullulans ferments to generate pullulans is remarkably improved, compared with the original strain, the yield of the pullulans is increased by 80%, and the glucose conversion rate is increased by 68%.The color of the finished pullulans becomes lighter obviously.Thus, the aureobasidium pullulans with the preservation number being CGMCC No.11937 can be widely applied to pullulans fermentation.
Owner:MEIHUA BIOTECH LANGFANG CO LTD
Cosmetic additive with anti-pollution effect and cosmetic thereof
The invention discloses a cosmetic additive with an anti-pollution effect and a cosmetic thereof, and aims to provide the cosmetic additive capable of effectively reducing adhesion to pollutants to the skin, protect skin cells, resisting external pollutants and having the effects of inflammation resisting, repairing and relieving. The cosmetic additive comprises, by weight, 3-12 parts of braseniaschreberi leaf extract, 1-10 parts of quercus robur root extract, 1-7 parts of moringa pterygosperma seed extract, 2-8 parts of salicornia herbacea extract, 1-6 parts of aureobasidium pullulans fermentation product and 2-7 parts of ginkgo biloba extract. The cosmetic additive can be used in cosmetics to enhance the skin barrier and achieve excellent isolation and defense functions on the skin andhas a good application prospect. The cosmetic belongs to the technical field of cosmetics.
Owner:GUANGDONG BAWEI BIOLOGICAL TECH CO LTD
Nitrogen, phosphorus and potassium microbial coated fertilizer and preparation method thereof
InactiveCN106187503AIncrease profitAvoid cakingSuperphosphatesAlkali orthophosphate fertiliserPullulanMicrobial agent
The invention discloses a nitrogen, phosphorus and potassium microbial coated fertilizer and a preparation method thereof. The nitrogen, phosphorus and potassium microbial coated fertilizer comprises a pill core, a middle layer and a mycoderm layer from inside to outside in sequence; the pill core comprises the following raw materials: urea, monopotassium phosphate, diammonium phosphate, calcium superphosphate, potassium sulphate, plant ash and glutinous rice glue; the middle layer comprises the following raw materials: furfural residues, vinasse, sorghum residues, edible fungus culture medium wastes, aureobasidium pullulans, EM (effective microorganism) bacterial liquid and clay; the mycoderm layer comprises the following raw materials: peat soil, a microbial agent, Carbomer, 10 wt% sodium hydroxide aqueous solution and water. The nitrogen, phosphorus and potassium microbial coated fertilizer disclosed by the invention has a viable count of more than 0.062 billion CFU / g, an organic content of more than 60.5 wt%, a pH value of 7.1-7.2 and an N, P and K content ratio of (2-3.5):(1.5-2.1):1; the reasonable collocation is performed according to nitrogen, phosphorus, potassium and other demands of crops, so that the release period of fertilizer nutrients can be prolonged; the microbial agent improves the soil texture and the tillage performance and inhibits the emergence of weeds and pests. The preparation method of the fertilizer has the advantages that the raw materials are easy to obtain, the operation is simple, the biological survival rate is high, the activity is good, and the use effect is outstanding.
Owner:SHANDONG ZHIFENG NONGHUA GRP
Method for producing novel natural melanin
ActiveCN102517335AIncrease productionIncrease profitMicroorganism based processesFermentationPullulanMelanin
The invention discloses a method for producing novel natural melanin. The method is characterized by comprising the following steps of: activating aureobasidium pullulans for 4 to 8 hours, performing shaking culture for 12 to 36 hours to obtain seed liquid, inoculating the seed liquid in a volume of which is 1 to 10 percent of that of a fermentation medium to the fermentation medium, and performing liquid state fermentation; centrifuging the fermentation solution obtained by fermentation for 5 minutes under the condition of 5,000r / min, performing acidolysis on the supernate by using hydrochloric acid, and then performing precipitation by using methanol to obtain a crude melanin product; dissolving the crude product to obtain a black solution by using an alkali solution; and performing precipitation on the obtained black solution by using hydrochloric acid, and thus obtaining the melanin. Compared with other fermentation medium, the method using the fermentation medium has the advantages that: the yield of the melanin is improved from 2.47g / L to 10.74g / L, the fermentation period is shortened from 7 days to 3 days, the culture substrate is fully utilized, the utilization rate of raw materials is improved, and the production cost is reduced.
Owner:天津北洋百川生物技术有限公司
Aureobasidium pullulans and applications thereof
ActiveCN107058123AGrowth inhibitionPromote growthFungiMicroorganism based processesBiotechnologyPullulan
The invention provides aureobasidium pullulans and applications thereof. The collection number of the strain is CGMCC No.12249, and marine yeast aureobasidium pullulans HJM01 is applied to agricultural production as a microbial fertilizer. The screened marine yeast aureobasidium pullulans and active substances in fermented liquor of the aureobasidium pullulans serve as the microbial fertilizer, the fertilizer can effectively inhibit and prevent the occurrence of diseases of botrytis cinerea on vegetables of tomatoes and the like after being applied to soil, the strain can decompose insoluble phosphate to produce soluble phosphate after producing acid in the soil, and phytase produced from the strain can decompose phytic acid in the soil to produce soluble phosphate, so that the soil fertility can be increased and the growth of plants can be promoted.
Owner:OCEAN UNIV OF CHINA
Aureobasidium pullulans and method for fermenting to produce pulullan thereof
ActiveCN107699500AIncrease productionReduce manufacturing costFungiMicroorganism based processesBiotechnologySnow mold
The invention belongs to the technical field of microbes and in particular relates to an aureobasidium pullulans stain NCPS 2016-M capable of producing pulullan at high yield. The aureobasidium pullulans stain NCPS 2016-M capable of producing pulullan at high yield provided by the invention is characterized in that the strain is collected in China Center for Type Culture Collection Center on September 14, 2017, and has a collection number of CCTCC M 2017498. The aureobasidium pullulans stain disclosed by the invention has the beneficial effects that the amount of the pulullan produced by performing shaking flask fermentation on the aureobasidium pullulans stain provided by the invention reaches 60g / L, and the ability of producing the pullulans is 1.5-1.7 times that of a wild aureobasidiumpullulans stain. The mutant strain does not produce any melanin, and the fermentation time is shortened by six hours compared with that of an original strain. The technical problem that wide application of the pullulans in the domestic food industry, particularly in an agricultural product preservation aspect, is greatly limited because the pullulans production level is low and the product qualityis low is solved.
Owner:INST OF AGRO FOOD SCI & TECH SHANDONG ACAD OF AGRI SCI
Aureobasidium pullulans, culturing medium and method for producing b-glucan, a culture of aureobasidium pullulans and a composition comprising the same
ActiveUS20180187226A1High yieldWithout melaninFungiMicroorganism based processesPullulanStaphylococcus cohnii
The present application provides a culture medium for producing β-glucan. The culture medium comprises: a carbon source, lecithin and an ascorbic acid. The present application further provides a method for producing β-glucan, the method comprising culturing Aureobasidium pullulans in the culture medium for fermentation. The Aureobasidium pullulans is advantageous in producing no melanin; as a result, the yield of β-glucan can be increased and the waste can be reduced with the improved culture medium composition and culturing method.
Owner:FOOD IND RES & DEV INST
Aureobasidium pullulans strain and application thereof
ActiveCN109136109AEfficient fermentationThe post-processing process is simpleFungiMicroorganism based processesMicroorganismPullulan
The invention relates to an Aureobasidium pullulans strain and an application thereof, and belongs to the technical fields of fermentation engineering and biology. The preservation name of the Aureobasidium pullulans strain is Aureobasidium pullulans ZK-502, the strain is preserved in CGMCC (China General Microbiological Culture Collection Center) on August 20, 2018 at No.3 of No.1 West Beichen Road, Chaoyang District, Beijing, and the preservation number is CGMCC No. 16326. The Aureobasidium pullulans strain can efficiently produce liamocin by fermentation and produces no melanin, fermentation broth is light pink to faint yellow, and follow-up treatment process can be simplified. The method is simple and feasible, raw materials are simple in composition, yield of liamocin can reach 8-10 g / L, and commercial and large-scale production of liamocin is expected to be realized; the obtained liamocin product is dissoluble in water and has wide application prospect in medicine, cosmetics andfood.
Owner:优鲜工坊(浙江)食品有限公司
Method for increasing yield of pullulan
InactiveCN105624233AIncrease productionIncrease production capacityFermentationBiotechnologyPullulan
The invention discloses a method for increasing the yield of pullulan. The method comprises the following steps: after activating aureobasidium pullulans, inoculating a seed medium; after OD600 is greater than 0.3, inoculating seeds into a fermentation medium; monitoring OD600 of a fermentation liquid in real time; when OD600 is greater than 0.2 and less than 0.3, adding a compounding agent I which accounts for 0.02-0.03% of the volume of the fermentation liquid, so as to greatly increase the bacterium growth velocity and remarkably shorten the logarithmic growth phase; when OD600 is greater than or equal to 0.6, adding a compounding agent II which accounts for 0.03-0.05% of the volume of the fermentation liquid. The substrate conversion speed can be accelerated, the polysaccharide synthesis performance can be improved, and the yield can be greatly increased.
Owner:天津北洋百川生物技术有限公司
Method for increasing yield of aureobasidium pullulans synthesized polymalic acid
InactiveCN105624218AIncrease productionImprove permeabilityMicroorganism based processesFermentationTemperature controlPullulan
The invention discloses a method for increasing the yield of aureobasidium pullulans synthesized polymalic acid. The method comprises the following steps: culturing seeds, and performing fermental cultivation, namely, adding an active agent consisting of CTTE and DMSO in the fermentation process in different stages, controlling the temperature of the fermentation process by using a stage temperature control method, setting the cultivation temperature to 25-35 DEG C for 0-40 hours, adjusting the temperature to 20-30 DEG C after 40 hours, flowing 0.1-0.14% of CTTE at a logarithmic growth stage in the fermentation process, and flowing 0.06-0.1% of DMSO at the middle fermentation stage. By the adoption of the method, the yield of polymalic acid is increased by improving permeability of cell membranes, the fermentation period is also shortened, the yield of polymalic acid is generally increased by 40%, the production cost is reduced, and the production efficiency is improved.
Owner:天津北洋百川生物技术有限公司
Method for preparing bio-adsorbent for chromium-containing wastewater treatment and application thereof
InactiveCN103585963AWide variety of sourcesLow costOther chemical processesWater/sewage treatment by sorptionAdjuvantPullulan
The invention belongs to the technical field of heavy-metal wastewater treatment and particularly relates to a method for preparing an aureobasidium pullulans fermented wheat-bran adsorbent and application of the adsorbent. The method specifically comprises the steps of crushing wheat bran, then, inoculating aureobasidium pullulans cell sap with the concentration of 5-15%, and carrying out solid-state fermentation for 48-120 hours at the temperature of 20-30 DEG C, thereby obtaining the heavy-metal bio-adsorbent which contains pullulan, aureobasidium pullulans thallus cells and fermented wheat bran residues. According to the application, the prepared adsorbent is applied to industrial wastewater treatment so as to remove heavy-metal chromium ions from wastewater, the chromium ion adsorbed wheat-bran adsorbent is applied to incineration power generation, meanwhile, heavy-metal chromium is separated and recovered from ash slag, and the incineration ash slag is applied to soil fertilization serving as an organic fertilizer adjuvant. According to the method and the application, not only is the efficient comprehensive utilization of agricultural waste resources realized, but also the method has the advantages that the raw materials are wide in source, are cheap and are easily obtained, the cost of the product is low, the process is simple, waste water and waste residues which pollute the environment are not caused, the adsorption capacity is large, the performance is stable, and the like.
Owner:射阳县芦苇开发有限公司
Biological fermentation preparation method of black garlic
ActiveCN103584006AShort manufacturing cycleIncrease nutritionFood dryingFood ingredient functionsPullulanDietary fiber
The invention discloses a preparation method of black garlic. The method is characterized in that aureobasidium pullulans fermented garlic is utilized to produce the black garlic. The method comprises the following basic processes: cleaning and drying the fresh and entire garlic in air; adding to 30 to 50g / L of sterilized wheat bran liquid; inoculating aureobasidium pullulans cell cap at a certain concentration; adjusting the temperature to reach 25 to 35 DEG C under pH of 5.0 to 6.0; fermenting for 3 to 6 days; heating to reach 70 to 80 DEG C; fermenting for 4 to 7 days; drying; and sieving to remove the fermented wheat bran to obtain the black garlic. The black garlic can be used as a health instant food or used together with seasoning; the fermented wheat bran can be used as a supplement of dietary fibers; or the black garlic and wheat bran can be dried and then crushed together and used as a nutrition supplement or used together with the seasoning. The preparation of the black garlic has the advantages that the preparation cycle is reduced; the nutrition of the black garlic is greatly improved with the mixing of wheat bran and aureobasidium pullulans fermentation, and the wheat bran dietary fiber is co-produced.
Owner:安徽张士奎食品有限公司
Mutant strain capable of realizing high-efficiency production of low-molecular pulullan and application thereof
ActiveCN107760608AEfficient productionIncrease production capacityFungiMicroorganism based processesBiotechnologyFermentation broth
The invention discloses a mutant strain capable of realizing high-efficiency production of low-molecular pulullan and application thereof, belonging to the technical field of mutagenesis of strains. The Aureobasidium pullulans UVMU6-1 capable of realizing high-efficiency production of low-molecular pulullan in the invention has an access number of CGMCC No.13178. The invention also provides application of the Aureobasidium pullulans UVMU6-1 to production of low-molecular pulullan. The strain provided by the invention has high pulullan production capability and can effectively improve the yieldof pulullan and reduce production cost; since the strain has low viscosity during fermentation, the concentration of produced pulullan in fermentation broth is high, and fermentation cost is effectively reduced; and a production method for producing pulullan via the strain is mild in conditions and friendly to environment, and the produced pulullan is green, natural, etc.
Owner:QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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