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Aureobasidium pullulans strain and application thereof

A technology of Aureobasidium pullulans and strains, applied in the direction of fungi, microorganisms, biochemical equipment and methods, etc., can solve the problems of clear research on liamocin biosynthesis mechanism, complex liamocin biosynthesis pathway, and low yield of liamocin, and achieve a simplified later stage The treatment process and method are simple and easy, and the extraction method is simple

Active Publication Date: 2019-01-04
优鲜工坊(浙江)食品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the biosynthetic pathway of liamocin is very complicated, and the biosynthetic mechanism of liamocin has not been studied clearly
Scholars represented by Kurosawa T and Price NPJ have done in-depth research on the structure, performance and application of liamocin, but there are few studies on the fermentation of liamocin, and the yield of liamocin in the current prior art is generally not high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] The method that Aureobasidium pullulans strain of the present invention is used for fermentation to produce liamocin is as follows:

[0046] S1. Activation of strains: transfer the slant bacteria to PDA slant medium, and cultivate them in a constant temperature incubator at 26°C for 2 days;

[0047]S2. Seed cultivation: select 3 loops of well-cultured slant strains and inoculate them in 50 mL of seed culture medium, and cultivate them in a shaker at 26°C and 160r / min for 2 days;

[0048] Among them, the composition of the seed medium is: 5% glucose, 0.2% yeast extract, 0.5% KH 2 PO 4 , 0.04% MgSO 4 ·7H 2 O, 0.04% MnSO 4 ·H 2 O and 0.1% NaCl.

[0049] S3, fermentation culture: divided into two stages

[0050] (1) Inoculate the seed liquid cultivated in step S2 into the fermentation medium (1) at a ratio of 6%, and cultivate it in a shaker at 26° C. and 160 r / min for 3 days;

[0051] Wherein, the composition of fermentation medium (1) is: 5% glucose, 0.2% yeast ex...

Embodiment 2

[0056] The method that Aureobasidium pullulans strain of the present invention is used for fermentation to produce liamocin is as follows:

[0057] S1. Strain activation: transfer the slant bacteria to the PDA slant medium, and cultivate them in a constant temperature incubator at 28°C for 2.5 days;

[0058] S2. Seed culture: select the well-cultured slant strains in step S1, pick 2 loops and inoculate them in 50 mL of seed culture medium, and cultivate them in a shaker at 26° C. and 160 r / min for 2 days;

[0059] Among them, the composition of the seed medium is: 5% glucose, 0.2% yeast extract, 0.5% KH 2 PO 4 , 0.04% MgSO 4 ·7H 2 O, 0.04% MnSO 4 ·H 2 O and 0.1% NaCl.

[0060] S3, fermentation culture: divided into two stages:

[0061] (1) Inoculate the seed solution cultivated in step S2 into the fermentation medium (1) at a ratio of 10%, and cultivate it in a shaker at 28° C. and 200 r / min for 3 days;

[0062] Among them, the composition of fermentation medium (1) is...

Embodiment 3

[0067] The method that Aureobasidium pullulans strain of the present invention is used for fermentation to produce liamocin is as follows:

[0068] S1. Activation of strains: transfer the slant bacteria to PDA slant medium, and cultivate them in a constant temperature incubator at 26°C for 2 days;

[0069] S2. Seed culture: select the slant bacteria cultured well in step S1, pick 3 loops and inoculate them in the seed culture medium, and cultivate them in a shaker at 28°C and 200r / min for 2 days;

[0070] Among them, the composition of the seed medium is: 5% glucose, 0.2% yeast extract, 0.5% KH 2 PO 4 , 0.04% MgSO 4 ·7H 2 O, 0.04% MnSO 4 ·H 2 O and 0.1% NaCl.

[0071] S3, fermentation culture: divided into two stages

[0072] (1) Inoculate the seed solution cultivated in step S2 into the fermentation medium (1) at a ratio of 8%, and cultivate it in a shaker at 28° C. and 180 r / min for 3 days;

[0073] Among them, the composition of the fermentation medium (1) is: 7% ma...

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Abstract

The invention relates to an Aureobasidium pullulans strain and an application thereof, and belongs to the technical fields of fermentation engineering and biology. The preservation name of the Aureobasidium pullulans strain is Aureobasidium pullulans ZK-502, the strain is preserved in CGMCC (China General Microbiological Culture Collection Center) on August 20, 2018 at No.3 of No.1 West Beichen Road, Chaoyang District, Beijing, and the preservation number is CGMCC No. 16326. The Aureobasidium pullulans strain can efficiently produce liamocin by fermentation and produces no melanin, fermentation broth is light pink to faint yellow, and follow-up treatment process can be simplified. The method is simple and feasible, raw materials are simple in composition, yield of liamocin can reach 8-10 g / L, and commercial and large-scale production of liamocin is expected to be realized; the obtained liamocin product is dissoluble in water and has wide application prospect in medicine, cosmetics andfood.

Description

technical field [0001] The invention relates to an Aureobasidium pullulans strain and an application thereof, belonging to the fields of fermentation engineering and biotechnology. Background technique [0002] Liamocin is one of the new active products of Aureobasidium pullulans. Because its density is heavier than water, it will settle at the bottom when it is still, so it is also called heavy oil. Liamocin currently generally refers to a class of polyol ester compounds that are heavier than water produced by Aureobasidium pullulans. The head group of the polyol ester is mostly mannitol or arabic alcohol, and the tail is a triplet of 3,5-dihydroxydecanoic acid. body or quadruple. [0003] Liamocin has a coconut aroma and is denser than water. The color of the oil varies from light yellow to malachite, and most of the oil is fluorescent. It is slightly soluble in water and easily soluble in organic solvents. It has been proved that the polyol ester has various biological ...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P7/62C12R1/645
CPCC12P7/62C12N1/145C12R2001/645
Inventor 魏培莲周丹凤吕磊磊刘士旺肖功年龚金炎柳永鲍文娜袁海娜
Owner 优鲜工坊(浙江)食品有限公司
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