Method for producing beta-polymalic acid by inert support adsorption solid-state fermentation

An inert carrier and solid-state fermentation technology, which is applied in the field of fermentation engineering to achieve the effects of small resistance, good hydrodynamic properties, and favorable mass and heat transfer

Inactive Publication Date: 2010-04-21
天津北洋百川生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, this fermentation method is still very immature, and many aspects are still blank. With the application of other solid-state fermentation technologies, such as pressure pulsation solid-state fermentation technology and computer process control technology in the solid-state fermentation process, this method will become more and more popular. Perfect, thus bringing a new development prospect to the fermentation industry

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1. Preparation of culture medium

[0021] (1) Strain Activation Medium: Potato Dextrose Agar Medium (PDA): Potato 200g / L, Glucose 20g / L, Agar 20g / L, pH4.0~4.5, 121°C autoclaved for 15min;

[0022] (2) Seed medium: glucose 8%, ammonium succinate 0.3%, succinic acid 0.2%, K 2 CO 3 0.04%, KH 2 PO 4 0.01%, MgSO 4 ·7H 2 O 0.01%, ZnSO 4 ·7H 2 O 5×10 -4 %, corn extract 0.1%, CaCO 3 2% (sterilized alone), sterilized by high-pressure steam at 121°C for 15 minutes;

[0023] (3) Fermentation medium: 5% sucrose, 0.3% ammonium succinate, 0.2% succinic acid, K 2 CO 3 0.04%, KH 2 PO 4 0.01%, MgSO 4 ·7H 2 O 0.01%, ZnSO 4 ·7H 2 O 5×10 -4 %, corn extract 0.05%, CaCO 3 3% (sterilized alone), sterilized by high-pressure steam at 121°C for 15 minutes;

[0024] 2. Activation of strains

[0025] The slant strain—Aureobasidium pullulan (Aureobasidium Pullulan, which has been preserved in the General Microbiology Center of China Microbiological Culture Collection Mana...

Embodiment 2

[0035] 1. Preparation of culture medium

[0036] (1) Strain Activation Medium: Potato Dextrose Agar Medium (PDA): Potato 200g / L, Glucose 20g / L, Agar 20g / L, pH 4.0-4.5, sterilized by autoclaving at 121°C for 15min;

[0037] (2) Seed medium: glucose 8%, ammonium succinate 0.3%, succinic acid 0.2%, K 2 CO 3 0.04%, KH 2 PO 4 0.01%, MgSO 4 ·7H 2 O 0.01%, ZnSO 4 ·7H 2 O 5×10 -4 %, corn extract 0.1%, CaCO 3 2% (sterilized alone), sterilized by high-pressure steam at 121°C for 15 minutes;

[0038] (3) Fermentation medium: 5% sucrose, 0.3% ammonium succinate, 0.2% succinic acid, K 2 CO 3 0.04%, KH 2 PO 4 0.01%, MgSO 4 ·7H 2 O 0.01%, ZnSO 4 ·7H 2 O 5×10 -4 %, corn extract 0.05%, CaCO 3 3% (sterilized alone), sterilized by high-pressure steam at 121°C for 15 minutes;

[0039] 2. Activation of strains

[0040] Transfer the slant strain—Aureobasidium Pullulan (Aureobasidium Pullulan) to PDA medium, activate and culture at 25°C for 3-5 days;

[0041] 3. Seed cult...

Embodiment 3

[0050] 1. Preparation of culture medium

[0051] (1) Strain Activation Medium: Potato Dextrose Agar Medium (PDA): Potato 200g / L, Glucose 20g / L, Agar 20g / L, pH4.0~4.5, 121°C autoclaved for 15min;

[0052] (2) Seed medium: glucose 8%, ammonium succinate 0.3%, succinic acid 0.2%, K 2 CO 3 0.04%, KH 2 PO 4 0.01%, MgSO 4 ·7H 2 O 0.01%, ZnSO 4 ·7H 2 O 5×10 -4 %, corn extract 0.1%, CaCO 3 2% (sterilized alone), sterilized by high-pressure steam at 121°C for 15 minutes;

[0053] (3) Fermentation medium: 5% sucrose, 0.3% ammonium succinate, 0.2% succinic acid, K 2 CO 3 0.04%, KH 2 PO 4 0.01%, MgSO 4 ·7H 2 O 0.01%, ZnSO 4 ·7H 2 O 5×10 -4 %, corn extract 0.05%, CaCO 3 3% (sterilized alone), sterilized by high-pressure steam at 121°C for 15 minutes;

[0054] 2. Activation of strains

[0055] Transfer the slant strain—Aureobasidium Pullulan (Aureobasidium Pullulan) to PDA medium, activate and culture at 25°C for 3-5 days;

[0056] 3. Seed culture

[0057] Selec...

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Abstract

The invention provides a novel method for producing beta-polymalic acid by the solid-state fermentation of microorganisms, and relates to a novel process of producing the beta-polymalic acid by using an inert adsorption composite material to the solid-state fermentation of aureobacidium pullulans(at present, the strain is stored in the general microorganism center of China Committee for Culture Collection of Microorganisms, and the CGMCC No. is 3337.). The method comprises the following steps of: selecting a nontoxic inert support which is good in physical water absorption and stable in nature and has a porous structure, evenly mixing the inert support and a liquid culture medium inoculated with the aureobacidium pullulans in a certain ratio, sending the mixture to a solid-state reactor for fermentation, extracting a substrate after the solid-state fermentation with water, and inspecting the content of the polymalic acid. By the method, the fermentation efficiency of the polymalic acid is improved, and the transfer efficiency of oxygen in the fermentation process is enhanced; at the same time, because the inert support can be recycled, the discharge of the solid wastes is greatly reduced; the utilization ratio of the fermentation equipment is high, the fermentation period is short, the products are easy to be separated and extracted, and the yield and purity of the polymalic acid are improved; and the operating cost is low, the economic benefits are obvious, and the industrialized production is facilitated.

Description

technical field [0001] The invention belongs to the technical field of fermentation engineering, and in particular relates to a cultivation method for applying an inert adsorption carrier to Aureobasidium pullulans for solid-state fermentation to produce β-polymalic acid. Background technique [0002] Polymalic acid [Poly (malic-acid) or Poly malate, referred to as PMLA] is a homopolymer polymer with malic acid as the only monomer, and is a new type of fully biodegradable polymer material. As an aliphatic polyester, PMLA is highly water-soluble, biocompatible, biodegradable, bioabsorbable, non-immunogenic and chemically derivatizable, and has strong uses in medicine and can As a drug carrier for cardiovascular and brain tumor research, it can also be used as biomedical materials such as microcapsule materials, surgical sutures, wounds, burn treatment bandages, etc., directly used in the human body, and can also be used in cosmetic products and food packaging, etc. Material....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/62C12R1/645
Inventor 乔长晟王凤荣钟堃蒋磊徐勇虎贾士儒
Owner 天津北洋百川生物技术有限公司
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